Protection induced by vaccinating sheep with a thiol‐binding extract ofHaemonchus contortusmembranes is associated with its protease components

Parasite Immunology - Tập 27 Số 4 - Trang 121-126 - 2005
David P. Knox1, S. K. Smith1, Diane L. Redmond1, William D. Smith1
1Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, UK

Tóm tắt

SUMMARYPrevious work has shown that a protein extract enriched for cysteine protease activity (TSBP) prepared from adultHaemonchus contortususing thiol sepharose affinity chromatography confers substantial protection against a single challenge infection. TSBP comprised proteases and other proteins. Here, TSBP were further fractionated using anion‐exchange chromatography and fractions pooled on the basis of containing (1) protease activity, (2) a prominent non‐protease peptide and (3) material which did not bind to the column. A protection trial showed that only the protease‐enriched material conferred protective immunity and this was comparable to that observed in a TSBP‐immunized positive control group. Immunization stimulated a marked IgG response with the IgG2 isotype predominating.

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Parasite Immunology

Tập 27 Số 4

121-126

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