Production, Optimization and Partial Characterization of Bacterial Cellulose from Gluconacetobacter xylinus TJU-D2
Tóm tắt
The aim of this work was to optimize and characteristic a bacterial cellulose (BC) produced by Gluconoacetobacter xylinus TJU-D2. An one-factor test was conducted, then response surface method was applied to optimize BC yield and discover the best production condition. Moreover, mechanical strength, water holding capacity, water absorption rate and water release rate of BC was determined. Based on Plackett-Burman experiment, glucose, ethanol and initial pH were found to be the most significant factors for BC production. Afterwards, effects of the three main factors on BC yield were further investigated by Box-Behnken design and the optimum composition was glucose 19.575 g/L, ethanol 3.85%, and initial pH 4.32. Optimum result showed that BC yield was increased to 4.82 ± 0.23 g/L in 8th-day fermentation, 1.46-fold higher than before. Tensile strength reveals a Young’s modulus of 18.64 ± 0.23 MPa and tensile strength of up to 22.34 ± 0.26 MPa. The strain produced a high level of BC with characteristics suitable for application in materials and medical areas.
Tài liệu tham khảo
Li, Y., Tian, C., Tian, H., Zhang, J., He, X., Ping, W., Lei, H.: Improvement of bacterial cellulose production by manipulating the metabolic pathways in which ethanol and sodium citrate involved. Appl. Microbiol. Biotechnol. 96(6), 1479–1487 (2012)
Huang, Y., Zhu, C., Yang, J., Nie, Y., Chen, C., Sun, D.: Recent advances in bacterial cellulose. Cellulose 21(1), 1–30 (2014)
Kuo, C.-H., Chen, J.-H., Liou, B.-K., Lee, C.-K.: Utilization of acetate buffer to improve bacterial cellulose production by Gluconacetobacter xylinus. Food Hydrocolloids 53, 98–103 (2016)
Peng, X., Li, C., Liu, J., Yi, Z., Han, Y.: Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season. Bioresour. Technol. 235, 389–395 (2017)
Zhong, C., Zhang, G.C., Liu, M., Zheng, X.T., Han, P.P., Jia, S.R.: Metabolic flux analysis of Gluconacetobacter xylinus for bacterial cellulose production. Appl. Microbiol. Biotechnol. 97(14), 6189–6199 (2013)
Vasconcelos, N.F., Feitosa, J.P.A., da Gama, F.M.P., Morais, J.P.S., Andrade, F.K., de Souza, M.D.S.M., de Freitas, M: Bacterial cellulose nanocrystals produced under different hydrolysis conditions: properties and morphological features. Carbohydr. Polym. 155, 425–433 (2016)
Dayal, M.S., Catchmark, J.M.: Mechanical and structural property analysis of bacterial cellulose composites. Carbohydr. Polym. 144, 447–454 (2016)
Cheng, Z., Yang, R., Liu, X., Chen, H.: Green synthesis of bacterial cellulose via acetic acid pre-hydrolysis liquor of agricultural corn stalk used as carbon source. Bioresour. Technol. 234, 8–17 (2017)
Mohammadkazemi, F., Azin, M., Ashori, A.: Production of bacterial cellulose using different carbon sources and culture media. Carbohydr. Polym. 117, 518–523 (2015)
Zeng, X., Small, D.P., Wan, W.: Statistical optimization of culture conditions for bacterial cellulose production by Acetobacter xylinum BPR 2001 from maple syrup. Carbohydr. Polym. 85(3), 506–513 (2011)
Santos, S.M., Carbajo, J.M., Quintana, E., Ibarra, D., Gomez, N., Ladero, M., Eugenio, M.E., Villar, J.C.: Characterization of purified bacterial cellulose focused on its use on paper restoration. Carbohydr. Polym. 116, 173–181 (2015)
Plackett, R.L., Burman, J.P.: The design of optimum multifactorial experiments. Biometrika 33(4), 305–325 (1946)
de Sousa, K.A., da Faheina Junior, G.S., de Azevedo, D.C.S., Pinto, G.A.S.: Optimization of cellulase production by Trichoderma strains using crude glycerol as a primary carbon source with a 24 full factorial design. Waste Biomass Valorization 9(3), 357–367 (2018)
Du, R., Zhao, F., Peng, Q., Zhou, Z., Han, Y.: Production and characterization of bacterial cellulose produced by Gluconacetobacter xylinus isolated from Chinese persimmon vinegar. Carbohydr. Polym. 194, 200–207 (2018)
Du, R., Xing, H., Yang, Y., Jiang, H., Zhou, Z., Han, Y.: Optimization, purification and structural characterization of a dextran produced by L. mesenteroides isolated from Chinese sauerkraut. Carbohydr. Polym. 174, 409–416 (2017)
Du, R., Xing, H., Zhou, Z., Han, Y.: Isolation, characterisation and fermentation optimisation of glucansucrase-producing Leuconostoc mesenteroides DRP105 from sauerkraut with improved preservation stability. Int. J. Food Sci. Technol. 52(12), 2522–2530 (2017)
Shezad, O., Khan, S., Khan, T., Park, J.K.: Physicochemical and mechanical characterization of bacterial cellulose produced with an excellent productivity in static conditions using a simple fed-batch cultivation strategy. Carbohydr. Polym. 82(1), 173–180 (2010)
Schrecker, S., Gostomski, P.: Determining the water holding capacity of microbial cellulose. Biotechnol. Lett. 27(19), 1435–1438 (2005)
Cakar, F., Katı, A., Özer, I., Demirbağ, D.D., Şahin, F., Aytekin, A.: Newly developed medium and strategy for bacterial cellulose production. Biochem. Eng. J. 92, 35–40 (2014)
Amin, M.C.I.M., Abadi, A.G., Katas, H.: Purification, characterization and comparative studies of spray-dried bacterial cellulose microparticles. Carbohydr. Polym. 99, 180–189 (2014)
Uzyol, H.K., Saçan, M.T.: Bacterial cellulose production by Komagataeibacter hansenii using algae-based glucose. Environ. Sci. Pollut. Res. 24(12), 11154–11162 (2017)
Goelzer, F., Faria-Tischer, P., Vitorino, J., Sierakowski, M.-R., Tischer, C.: Production and characterization of nanospheres of bacterial cellulose from Acetobacter xylinum from processed rice bark. Mater. Sci. Eng. C 29(2), 546–551 (2009)
Gomes, F.P., Silva, N.H., Trovatti, E., Serafim, L.S., Duarte, M.F., Silvestre, A.J., Neto, C.P., Freire, C.S.: Production of bacterial cellulose by Gluconacetobacter sacchari using dry olive mill residue. Biomass Bioenergy 55, 205–211 (2013)
Hutchens, S.A., Leon, R., O’Neill, H.M., Evans, B.R.: Statistical analysis of optimal culture conditions for Gluconacetobacter hansenii cellulose production. Lett. Appl. Microbiol. 44(2), 175–180 (2007)
Sirohi, R., Singh, A., Tarafdar, A., Shahi, N.C., Verma, A.K., Kushwaha, A.: Cellulase production from pre-treated pea hulls using Trichoderma reesei under submerged fermentation. Waste Biomass Valorization (2018). https://doi.org/10.1007/s12649-018-0271-4
Yunoki, S., Osada, Y., Kono, H., Takai, M.: Role of ethanol in improvement of bacterial cellulose production: analysis using 13C-labeled carbon sources. Food Sci. Technol. Res. 10(3), 307–313 (2007)
Aydın, Y.A., Aksoy, N.D.: Isolation and characterization of an efficient bacterial cellulose producer strain in agitated culture: Gluconacetobacter hansenii P2A. Appl. Microbiol. Biotechnol. 98(3), 1065–1075 (2014)
Nguyen, V.T., Flanagan, B., Gidley, M.J., Dykes, G.A.: Characterization of cellulose production by a Gluconacetobacter xylinus strain from Kombucha. Curr. Microbiol. 57(5), 449–458 (2008)
Mohite, B.V., Patil, S.V.: Physical, structural, mechanical and thermal characterization of bacterial cellulose by G. hansenii NCIM 2529. Carbohydr. Polym. 106, 132–141 (2014)
Watanabe, K., Hori, Y., Tabuchi, M., Morinaga, Y., Yoshinaga, F., Horii, F., Sugiyama, J., Okano, T.: Structural features of bacterial cellulose vary depending on the cultural conditions. In: Proceedings of Cellulose Society of Japan, Kyoto, Japan, pp. 45–50 (1994)
Ciechanska, D.: Multifunctional bacterial cellulose/chitosan composite materials for medical applications. Fibres Text East Eur. 12(4), 69–72 (2004)
Vandamme, E., De Baets, S., Vanbaelen, A., Joris, K., De Wulf, P.: Improved production of bacterial cellulose and its application potential. Polym. Degrad. Stab. 59(1–3), 93–99 (1998)
Ul-Islam, M., Khan, T., Park, J.K.: Water holding and release properties of bacterial cellulose obtained by in situ and ex situ modification. Carbohydr. Polym. 88(2), 596–603 (2012)
Klemm, D., Schumann, D., Udhardt, U., Marsch, S.: Bacterial synthesized cellulose-artificial blood vessels for microsurgery. Prog. Polym. Sci. 26(9), 1561–1603 (2001)