Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

American Association for the Advancement of Science (AAAS) - Tập 239 Số 4839 - Trang 487-491 - 1988
Randall K. Saiki1, David H. Gelfand2, Susanne Stoffel2, Stephen J. Scharf1, Russell Higuchi1, Glenn T. Horn1, Kary B. Mullis1, Henry A. Erlich1
1Cetus Corporation, Department of Human Genetics, Emeryville, CA 94608.
2Cetus Corporation, Department of Microbial Genetics, Emeryville, CA 94608.

Tóm tắt

A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus , greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10 5 cells.

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American Association for the Advancement of Science (AAAS)

Tập 239 Số 4839

487-491

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