Phospholipase Dα1-derived phosphatidic acid interacts with ABI1 phosphatase 2C and regulates abscisic acid signaling

Proceedings of the National Academy of Sciences of the United States of America - Tập 101 Số 25 - Trang 9508-9513 - 2004
Wenhua Zhang1, Chunbo Qin1, Jian Zhao1, Xuemin Wang1
1Department of Biochemistry, Kansas State University, Manhattan, KS 66506

Tóm tắt

Phospholipase D (PLD) and protein phosphatase 2C (PP2C) both play a role in mediating plant responses to abscisic acid (ABA). In this article, we show that PLDα1 and its product, phosphatidic acid (PA), regulate a PP2C, ABI1, which is a negative regulator of ABA responses in Arabidopsis . Leaves from a T-DNA insertional mutant of PLD α 1 and PLD α 1 -antisense plants lose more water than do wild-type plants. The stomatal closure of PLD α 1 -null leaves is insensitive to ABA but is promoted by PA. ABA treatment promotes an increase in PA from phosphatidylcholine in wild type but not in PLD α 1 -null cells. PLDα1-derived PA binds to ABI1; the PA–ABI1 binding is demonstrated by coprecipitating PA with ABI1 from plant cells, measuring binding of PA from vesicles to ABI1, and assaying ABI1 bound to PA immobilized on a filter. Deletion and site-specific mutational analyses show that arginine 73 in ABI1 is essential for PA–ABI1 binding. PA binding decreases the phosphatase activity of ABI1. The lack of ABA-induced production of PA in PLD α 1 -null cells results in a decrease in the association of ABI1 with the plasma membrane in response to ABA. These results indicate that PA produced by PLDα1 inhibits the function of the negative regulator ABI1, thus promoting ABA signaling. The identification of ABI1 as a direct target of the lipid messenger PA provides a functional link between the two families of important signaling enzymes, PLD and PP2C.

Từ khóa


Tài liệu tham khảo

10.1105/tpc.010441

10.1073/pnas.242607499

10.1073/pnas.95.3.975

10.1126/science.287.5451.300

10.1146/annurev.arplant.52.1.627

10.1105/tpc.11.10.1897

10.1046/j.1365-313x.2001.00965.x

10.1046/j.1365-313X.2001.01048.x

10.1093/emboj/cdf316

10.1073/pnas.2034853100

Chérel, I., Michard, E., Platet, N., Mouline, K., Alcon, C., Sentenac, H. & Thibaud, J. B. (2001) Plant Cell 14, 1133-1146.

10.1093/jxb/52.354.181

10.1073/pnas.95.5.2697

10.1073/pnas.96.21.12192

10.1016/j.pbi.2004.03.012

10.1146/annurev.arplant.54.031902.134748

10.1104/pp.010928

10.1046/j.1365-313X.2001.01138.x

10.1126/science.1083695

10.1105/tpc.013961

Li, W., Li, M., Zhang, W., Welti, R. & Wang, X. (2004) Nat. Biotechnol. 24, 427-433.

Fan, L., Zheng, S. & Wang, X. (1997) Plant Cell 9, 2183-2196.9437863

10.1105/tpc.010210

10.1074/jbc.273.35.22761

10.1021/bi00631a035

10.1074/jbc.M205375200

10.1105/tpc.019000

10.1016/S0014-5793(02)03483-X

10.1074/jbc.M302933200

10.1104/pp.004002

10.1074/jbc.M300878200

10.1007/s00425-001-0675-3

10.1074/jbc.M111555200

Pei, Z. M., Murata, Y., Benning, G., Thomine, S., Klusener, B., Allen, G. J., Grill, E. & Schroeder, J. I. (2000) Nature 46, 731-734.

10.1046/j.1365-313X.1999.00608.x

10.1104/pp.126.4.1449

10.1016/S0005-2760(98)00048-4

10.1105/tpc.12.11.2237

10.1074/jbc.M309529200

10.1126/science.1059046

10.1046/j.1365-313x.2001.01037.x

10.1038/sj.emboj.7600068

Thông tin
Thông tin xuất bản

Proceedings of the National Academy of Sciences of the United States of America

Tập 101 Số 25

9508-9513

Thông tin tác giả