PSF là một protein liên kết với IbeA góp phần vào sự xâm nhập của Escherichia coli K1 gây viêm màng não vào tế bào nội mạch vi não của người

Springer Science and Business Media LLC - Tập 196 - Trang 135-143 - 2007
Yanming Zou1, Lina He1, Chun-Hua Wu1, Hong Cao2, Zhi-Hua Xie3, Yannan Ouyang1, Yang Wang1, Ambrose Jong1, Sheng-He Huang1,2,4
1Saban Research Institute, Childrens Hospital Los Angeles, Los Angeles, USA
2Department of Microbiology, Southern Medical University, Guangzhou, China
3ProteinX Lab, San Diego, USA
4Department of Pediatrics, Childrens Hospital Los Angeles, University of Southern California, Los Angeles, USA

Tóm tắt

Trong quá trình phát triển của bệnh viêm màng não do Escherichia coli K1, sự tương tác giữa protein xâm nhập E. coli IbeA và protein bề mặt trên tế bào nội mạch vi não của người (HBMEC) là cần thiết cho quá trình xâm nhập và tín hiệu trung gian IbeA. Trong nghiên cứu này, một protein liên kết với IbeA được xác định là yếu tố splicing liên kết với protein polypyrimidine tract-binding (PTB). Sự liên kết cụ thể được xác nhận qua phương pháp thử nghiệm ligand overlay. Protein PSF biểu hiện trên bề mặt tế bào đã được xác minh qua kính hiển vi huỳnh quang. PSF tái tổ hợp đã hiệu quả ngăn chặn sự xâm nhập của E. coli K1 vào HBMEC. Việc quá biểu hiện PSF trong tế bào HBMEC truyền qua lentivirus đã làm tăng đáng kể sự xâm nhập của E. coli K1. Những kết quả này gợi ý rằng IbeA tương tác với PSF để xâm nhập E. coli K1 vào HBMEC.

Từ khóa

#Escherichia coli K1 #IbeA #protein liên kết #PSF #tế bào nội mạch vi não #xâm nhập

Tài liệu tham khảo

Akhmedov AT, Lopez BS (2000) Human 100-kDa homologous DNA-pairing protein is the splicing factor PSF and promotes DNA strand invasion. Nucleic Acids Res 28:3022–3030 Badger JL, Wass CA, Weissman SJ, Kim KS (2000) Application of signature-tagged mutagenesis for identification of Escherichia coli K1 genes that contribute to invasion of human brain microvascular endothelial cells. Infect Immun 68:5056–5061 Bladen CL, Udayakumar D, Takeda Y, Dynan WS (2005) Identification of the polypyrimidine tract binding protein-associated splicing factor p54(nrb) complex as a candidate DNA double-strand break rejoining factor. J Biol Chem 280:5205–5210 Bonacorsi S, Clermont O, Houdouin W, Cordevant C, Brahimi N, Marecat A, Tinsley C, Nassif X, Lange M, Bingen E (2003) Molecular analysis and experimental virulence of french and North American Escherichia coli neonatal meningitis isolates: Identification of a new virulent clone. J Infect Dis 187:1895–1906 Braun L, Ghebrehiwet B, Cossart P (2000) gC1q-R/p32, a C1q-binding protein, is a receptor for the InlB invasion protein of Listeria monocytogenes. EMBO J 19:1458–1466 Chen M, Li W, Fan J, Kasahara N, Woodley D (2003) An efficient gene transduction system for studying gene function in primary human dermal fibroblasts and epidermal keratinocytes. Clin Exp Dermatol 28:193–199 Chen YH, Chen SHM, Jong A, Zhou ZY, Li W, Suzuki K, Huang SH (2002) Enhanced Escherichia coli invasion of human brain microvascular endothelial cells is associated with alternations in cytoskeleton induced by nicotine. Cell Microbiol 4:503–514 Chung JW, Hong SJ, Kim KJ, Goti D, Stins MF, Shin S, Dawson VL, Dawson TM, Kim KS (2003) 37-kDa laminin receptor precursor modulates cytotoxic necrotizing factor 1-mediated RhoA activation and bacterial uptake. J Biol Chem 278:16857–16862 Dye BT, Patton JG (2001) An RNA recognition motif (RRM) is required for the localization of PTB-associated splicing factor (PSF) to subnuclear speckles. Exp Cell Res 263:131–144 Gower HJ, Moore SE, Dickson G, Elsom VL, Nayak R, Walsh FS (1989) Cloning and characterization of a myoblast cell-surface antigen defined by 24.1D5 monoclonal-antibody. Development 105:723–731 Hoffman JA, Badger JL, Zhang Y, Huang SH, Kim KS (2000) Escherichia coli K1 aslA contributes to invasion of brain microvascular endothelial cells in vitro and in vivo. Infect Immun 68:5062–5067 Huang SH, Chen YH, Fu Q, Stins M, Wang Y, Wass C, Kim KS (1999) Identification and characterization of an Escherichia coli invasion gene locus, ibeB, required for penetration of brain microvascular endothelial cells. Infect Immun 67:2103–2109 Huang SH, Jong AY (2001) Cellular mechanisms of microbial proteins contributing to invasion of the blood–brain barrier. Cell Microbiol 3:277–287 Huang SH, Stins MF, Kim KS (2000) Bacterial penetration across the blood-brain barrier during the development of neonatal meningitis. Microb Infect 2:1237–1244 Huang SH, Wan ZS, Chen YH, Jong AY, Kim KS (2001) Further characterization of Escherichia coli brain microvascular endothelial cell invasion gene ibeA by deletion, complementation, and protein expression. J Infect Dis 183:1071–1078 Kim KJ, Chung JW, Kim KS (2005) 67-kDa laminin receptor promotes internalization of cytotoxic necrotizing factor 1-expressing Escherichia coli K1 into human brain microvascular endothelial cells. J Biol Chem 280:1360–1368 Kim KS (2003) Pathogenesis of bacterial meningitis: from bacteraemia to neuronal injury. Nat Rev Neurosci 4:376–385 Kim KS, Itabashi H, Gemski P, Sadoff J, Warren RL, Cross AS (1992) The K1 capsule is the critical determinant in the development of Escherichia coli meningitis in the rat. J Clin Invest 90:897–905 Patton JG, Porro EB, Galceran J, Tempst P, Nadalginard B (1993) Cloning and characterization of PSF, a novel premessenger RNA splicing factor. Gen Develop 7:393–406 Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG (2002) PSF and p54(nrb) bind a conserved stem in U5 snRNA. RNA 8:1334–1347 Prasadarao NV, Srivastava PK, Rudrabhatla RS, Kim KS, Huang SH, Sukumaran SK (2003) Cloning and expression of the Escherichia coli K1 outer membrane protein A receptor, a gp96 homologue. Infect Immun 71:1680–1688 Prasadarao NV, Wass CA, Hacker J, Jann K, Kim KS (1993) Adhesion of S-fimbriated Escherichia coli to brain glycolipids mediated by SfaA gene-encoded protein of S-fimbriae. J Biol Chem 268:10356–10363 Prasadarao NV, Wass CA, Kim KS (1997) Identification and characterization of S Fimbria-binding sialoglycoproteins on brain microvascular endothelial cells. Infect Immun 65:2852–2860 Prasadarao NV, Wass CA, Stins MF, Shimada H, Kim KS (1999) Outer membrane protein A-promoted actin condensation of brain microvascular endothelial cells is required for Escherichia coli invasion. Infect Immun 67:5775–5783 Rosenberger U, Lehmann I, Weise C, Franke P, Hucho F, Buchner K (2002) Identification of PSF as a protein kinase C alpha-binding protein in the cell nucleus. J Cell Biochem 86:394–402 Shav-Tal Y, Cohen M, Lapter S, Dye B, Patton JG, Vandekerckhove J, Zipori D (2001) Nuclear relocalization of the pre-mRNA splicing factor PSF during apoptosis involves hyperphosphorylation, masking of antigenic epitopes, and changes in protein interactions. Mol Biol Cell 12:2328–2340 Shav-Tal Y, Zipori D (2002) PSF and p54(nrb)/NonO—multi-functional nuclear proteins. FEBS Lett 531:109–114 Sinclair JF, O’Brien AD (2002) Cell surface-localized nucleolin is a eukaryotic receptor for the adhesin intimin-gamma of enterohemorrhagic Escherichia coli O157 : H7. J Biol Chem 277:2876–2885 Srivastava M, Pollard HB (1999) Molecular dissection of nucleolin’s role in growth and cell proliferation: new insights. FASEB J 13:1911–1922 Stins MF, Prasadarao NV, Ibric L, Wass CA, Luckett P, Kim KS (1994) Binding characteristics of S-fimbriated Escherichia coli to isolated brain microvascular endothelial cells. Am J Pathol 145:1228–1236 Straub T, Grue P, Uhse A, Lisby M, Knudsen BR, Tange TO, Westergaard O, Boege F (1998) The RNA-splicing factor PSF/p54(nrb) controls DNA-topoisomerase I activity by a direct interaction. J Biol Chem 273:26261–26264 Wang Y (2002) The function of OmpA in Escherichia coli. Biochem Biophys Res Commun 292:396–401 Wang Y, Huang SH, Wass CA, Stins MF, Kim KS (1999) The gene locus yijP contributes to Escherichia coli K1 invasion of brain microvascular endothelial cells. Infect Immun 67:4751–4756 Watt S, Lanotte P, Mereghetti L, Moulin-Schouleur M, Picard B, Quentin R (2003) Escherichia coli strains from pregnant women and neonates: intraspecies genetic distribution and prevalence of virulence factors. J Clin Microbiol 41:1929–1935 Weiser JN, Gotschlich EC (1991) Outer membrane protein A (OmpA) contributes to serum resistance and pathogenicity of Escherichia coli K-1. Infect Immun 59:2252–2258 Zhang JR, Tuomanen E (1999) Molecular and cellular mechanisms for microbial entry into the CNS. J Neurovirol 5:591–603 Zolotukhin AS, Michalowski D, Bear J, Smulevitch SV, Traish AM, Peng R, Patton J, Shatsky IN, Felber BK (2003) PSF acts through the human immunodeficiency virus type 1 mRNA instability elements to regulate virus expression. Mol Cell Biol 23:6618–6630