Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems
Tóm tắt
Từ khóa
Tài liệu tham khảo
Amann E, Brosius J, Ptashne M (1983) Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25:167–178
Anna DF, Rosa M, Emilia P, Simonetta B, Mose R (2003) High-level expression of Aliciclobacillus acidocaldarius thioredoxin in Pichiapastoris and Bacillus subtilis. Protein Expr Purif 30:179–184
Arechaga I, Miroux B, Karrasch S, Huijbregts R, De Kruiff B, Runswick MJ, Walker JE (2000) Characterisation of new intracellular membranes in Escherichia coli accompanying large scale over-production of the b subunit of F1F0 ATP synthase. FEBS Lett 482:215–219
Armarego WL, Cotton RG, Dahl HH, Dixon NE (1989) High-level expression of human dihydropteridine reductase (EC 1.6.99.7), without N-terminal amino acid protection, in Escherichia coli. Biochem J 261:265–268
Awram P, Smit J (1998) The Caulobacter crescentus paracrystalline S-layer protein is secreted by an ABC transporter (type I) secretion apparatus. J Bacteriol 180:3062–3069
Barnard GC, Henderson GE, Srinivasan S, Gerngross TU (2004) High level recombinant protein expression in Ralstonia eutropha using T7 RNA polymerase based amplification. Protein Expr Purif 38:264–271
Bessette PH, Aslund F, Beckwith J, Georgiou G (1999) Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm. Proc Natl Acad Sci USA 96:13703–13708
Bingle WH, Nomellini JF, Smit J (2000) Secretion of the Caulobacter crescentus S-layer protein: Further localization of the C-terminal secretion signal and its use for secretion of recombinant proteins. J Bacteriol 182:3298–3301
Braaz R, Wong SL, Jendrossek D (2002) Production of PHA depolymerase A (PhaZ5 from Paucimonas lemoignei in Bacillus subtilis. FEMS Microbiol Lett 209:237–241
Brawner ME (1994) Advances in heterologous gene expression by Streptomyces. Curr Opin Biotechnol 5:475–481
Brosius J, Erfle M, Storella J (1985) Spacing of the −10 and −35 regions in the tac promoter. Effect on its in vivo activity. J Biol Chem 260:3539–3541
Burger S, Tatge H, Hofmann F, Genth H, Just I, Gerhard R (2003) Expression of recombinant Clostridium difficile toxin A using the Bacillus megaterium system. Biochem Biophys Res Commun 307:584–588
Cho HY, Yukawa H, Inui M, Doi RH, Wong S-L (2004) Production of minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800. Appl Environ Microbiol 70:5704–5707
Choi JH, Lee SY (2004) Secretory and extracellular production of recombinant proteins using Escherichia coli. Appl Microbiol Biotechnol 64:625–635
Daguer JP, Chamber R, Petit-Glatron MF (2005) Increasing the stability of sacB transcript improves levansucrase production in Bacillus subtilis. Lett Appl Microbiol 41:221–226
De Boer HA, Comstock LJ, Vasser M (1983) The tac promoter: a functional hybrid derived from the trp and lac promoters. Proc Natl Acad Sci USA 80:21–25
Degenkolb J, Takahashi M, Ellestad GA, Hillen W (1991) Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor. Antimicrob Agents Chemother 35:1591–1595
DeLisa MP, Li J, Rao R, Weigand WA, Bentley WE (1999) Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor. Biotechnol Bioeng 65:54–64
Desplanq D, Bernard C, Sibler AP, Kieffer B, Miguet L, Potier N, Van Dorsselaer A, Weiss E (2005) Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120. Biotechniques 39:405–411
Dong H, Nilsson L, Kurland CG (1996) Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rate. J Mol Biol 260:649–663
Dubendorff JW, Studier FW (1991) Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. J Mol Biol 219:45–59
Elvin CM, Thompson PR, Argall ME, Hendry P, Stamford NP, Lilley PE, Dixon NE (1990) Modified bacteriophage lambda promoter for overproduction of proteins in Escherichia coli. Gene 87:123–126
England DF, Penfold RJ, Delaney SF, Rogers PL (1997) Isolation of Bacillus megaterium mutants that produce high levels of heterologous protein, and their use to construct a highly mosquitocidal strain. Curr Microbiol 35:71–76
FitzGerald KA, Lidstrom ME (2003) Overexpression of a heterologous protein, haloalkane dehalogenase, in a poly-beta-hydroxybutyrate-deficient strain of the facultative methylotroph Methylobacterium extorquens AM1. Biotechnol Bioeng 81:263–268
Geissendörfer M, Hillen W (1990) regulated expression of heterologous genes in Bacillus subtilis using the Tn10 encoded tet regulatory elements. Appl Microbiol Biotechnol 33:657–663
Georgiou G, Segatori L (2005) Preparative expression of secreted proteins in bacteria: status report and future prospects. Curr Opin Biotechnol 16:538–545
Goldman E, Rosenberg AH, Zubay G, Studier FW (1995) Consecutive low-usage leucine codons block translation only when near the 5′ end of a message in Escherichia coli. J Mol Biol 245:467–473
Gronenborn (1976) Overproduction of phage lambda repressor under control of the lac promotor of Escherichia coli. Mol Gen Genet 148:243–250
Grossman TH, Kawasaki ES, Punreddy SR, Osburne MS (1998) Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. Gene 209:95–103
Gutierrez J, Bourque D, Criado R, Choi YJ, Cintas LM, Hernandez PE, Miguez CB (2005) Heterologous extracellular production of enterocin P from Enterococcus faecium P13 in the methylotrophic bacterium Methylobacterium extorquens. FEMS Microbiol Lett 248:125–131
Guzman LM, Belin D, Carson MJ, Beckwith J (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 177:4121–4130
Haldimann A, Daniels L, Wanner B (1998) Use of new methods for construction of tightly regulated arabinose and rhamnose promotor fusions in studies of the Escherichia coli phosphate regulon. J Bacteriol 180:1277–1286
Hansson M, Samuelson P, Nguyen TN, Stahl S (2002) General expression vectors for Staphylococcus carnosus enabled efficient production of the outer membrane protein A of Klebsiella pneumoniae. FEMS Microbiol Lett 210:263–270
Henner DJ (1990) Inducible expression of regulatory genes in Bacillus subtilis. Methods Enzymol 185:223–228
Hockney RC (1994) Recent developments in heterologous protein production in Escherichia coli. Trends Biotechnol 12:456–463
Horinouchi S, Weisblum B (1982) Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies. J Bacteriol 150:804–814
Huang HC, Sherman MY, Kandror O, Goldberg AL (2001) The molecular chaperone DnaJ is required for the degradation of a soluble abnormal protein in Escherichia coli. J Biol Chem 276:3920–3928
Ichikawa Y, Yamagata H, Tochikubo K, Udaka S (1993) Very efficient extracellular production of cholera toxin B subunit using Bacillus brevis. FEMS Microbiol Lett 111:219–224
Inoue Y, Ohta T, Tada H, Iwasa S, Udaka S, Yamagata H (1997) Efficient production of a functional mouse/human chimeric Fab′ against human urokinase-type plasminogen activator by Bacillus brevis. Appl Microbiol Biotechnol 48:487–492
Joly JC, Swartz JR (1994) Protein folding activities of Eschrichia coli protein disulfide isomerase. Biochemistry 12:4231–4236
Kajino T, Saito Y, Hirai M, Asami O, Yamada Y, Udaka S (1997) Extracellular production of an intact and biologically active human growth hormone by the Bacillus brevis system. J Ind Microbiol Biotech 19:227–231
Kajino T, Ohto C, Muramatsu M, Obata S, Udaka S, Yamada Y, Takahashi H (1999) A protein disulfid isolerase gene fusion expression system that increases the extracellular productivity of Bacillus brevis. Appl Environ Microbiol 66:638–642
Kajino T, Takahashi H, Hirai M, Yamada Y (2000) Efficient production of artificially designed gelatins with a Bacillus brevis system. Appl Environ Microbiol 66:304–309
Kane JF (1995) Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr Opin Biotechnol 6:494–500
Kashima Y, Udaka S (2004) High-level production of hyperthermophilic cellulase in the Bacillus brevis expression and secretion system. Biosci Biotechnol Biochem 68:235–237
Kawamura F, Doi RH (1984) Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases. J Bacteriol 160:442–444
Kim L, Mogk A, Schumann W (1996) A xylose-inducible Bacillus subtilis interation vector and its application. Gene 181:71–76
Konishi H, Sato T, Yamagata H, Udaka S (1990) Efficient production of human alpha-amylase by a Bacillus brevis mutant. Appl Microbiol Biotechnol 34:297–302
Korpela MT, Kurittu JS, Karvinen JT, Karp MT (1998) A recombinant Escherichia coli sensor strain for the detection of tetracyclines. Anal Chem 70:4457–4462
Kurland C, Gallant J (1996) Errors of heterologous protein expression. Curr Opin Biotechnol 7:489–493
Lam KH, Chow KC, Wong WK (1998) Construction of an efficient Bacillus subtilis system for extracellular production of heterologous proteins. J Biotechnol 63:167–177
Landry TD, Chew L, Davis JW, Frawley N, Foley HH, Stelman SJ, Thomas J, Wolt J, Hanselmann DS (2003) Safety evaluation of an α-amylase enzyme preparation derived from the archaeal order Thermococcales as expressed in Pseudomonas fluorescens biovar I. Regul Toxicol Pharmacol 37:149–168
Le Grice SF (1990) Regulated promoter for high-level expression of heterologous genes in Bacillus subtilis. Methods Enzymol 185:201–214
Lesuisse E, Schanck K, Colson C (1993) Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extreme basic pH-tolerant enzyme. Eur J Biochem 216:155–160
Li W, Zhou X, Lu P (2004) Bottlenecks in the expression and secretion of heterologous proteins in Bacillus subtilis. Res Microbiol 155:605–610
Love CA, Lilley PE, Dixon NE (1996) Stable high-copy-number of bacteriophage lambda promoter vectors for overproduction of proteins in Escherichia coli. Gene 176:49–53
Lowman HB, Bina M (1990) Temperature-mediated regulation and downstream inducible selection for controlling gene expression from the bacteriophage lambda pL promoter. Gene 96:133–136
Lutz R, Bujard H (1997) Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC7l1–l2 regulatory elements. Nucleic Acids Res 25:1203–1210
Makrides SC (1996) Strategies for achieving high level expression of genes in Escherichia coli. Microbiol Rev 60:512–538
Malten M, Hollmann R, Deckwer WD, Jahn D (2005) Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium. Biotechnol Bioeng 89:206–218
Margot P, Karamata D (1996) The wrpA gene Bacillus subtilis 168 expressed during exponential growth, encodes a cell-wall-associated protease. Microbiology 142:3437–3444
Mayer M (1995) A new set of useful cloning and expression vectors derived from pBlueScript. Gene 163:41–46
McKenzie T, Hoshino T, Tanaka T, Sueoka N (1986) The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation. Plasmid 15:93–103
Meinhardt F, Stahl U, Eveling W (1989) Highly efficient expression of homologous and heterologous genes in Bacillus megaterium. Appl Microbiol Biotechnol 30:343–350
Menart V, Jevsevar S, Vilar M, Trobis A, Pavko A (2003) Constitutive versus thermoinducible expression of heterologous proteins in Escherichia coli based on strong PR,PL promoters from phage lambda. Biotechnol Bioeng 83:181–190
Mergulhao FJ, Monteiro GA (2004) Secretion capacity limitations of the Sec pathway in Escherichia coli. J Microb Biotechnol 14:128–133
Mergulhao FJ, Summers DK, Monteiro GA (2005) Recombinant protein secretion in Escherichia coli. Biotechnol Adv 23:177–202
Miroux B, Walker JE (1996) Over-production of proteins in Escherichia coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high level. J Mol Biol 260:289–298
Moffatt BA, Studier FW (1987) T7 lysozyme inhibits transcription by T7 RNA polymerase. Cell 49:221–227
Murashima K, Chen C-L, Kosugi A, Tamaru Y, Doi RH, Wong S-L (2002) Heterologous production of Clostridium cellulovorans engB, using protease-deficient Bacillus subtilis, and preparation of active recombinant cellulosomes. J Bacteriol 184:76–81
Nakamura K, Furusato T, Shiroza T, Yamane K (1985) Stable hyper-production of Escherichia coli β-lactamase by Bacillus subtilis grown on a 0.5 M succinate-medium using a B. subtilis α-amylase secretion vector. Biochem Biophys Res Commun 128:601–606
Olmos-Soto J, Contreras-Flores R (2003) Genetic system constructed to overproduce and secrete proinsulin in Bacillus subtilis. Appl Microbiol Biotechnol 62:369–373
Palva I (1982) Molecular cloning of alpha-amylase gene from Bacillus amyloliquefaciens and its expression in B. subtilis. Gene 19:81–87
Palva I, Lehtovaara P, Kaariainen L, Sibakov M, Cantell K, Schein CH, Kashiwagi K, Weissmann C (1983) Secretion of interferon by Bacillus subtilis. Gene 22:229–235
Pan SH, Malcolm BA (2000) Reduced background expression and improved plasmid stability with pET vectors in BL21 (DE3). Biotechniques 29:1234–1238
Phillips TA, Van Bogelen RA, Neidhardt FC (1984) lon gene product of Escherichia coli is a heat-shock protein. J Bacteriol 159:283–287
Polisky B, Bishop RJ, Gelfand DH (1976) A plasmid cloning vehicle allowing regulated expression of eukaryotic DNA in bacteria. Proc Natl Acad Sci USA 73:3900–3904
Puyet A, Sandoval H, Lopez P, Aguilar A, Martin JF, Espinosa M (1987) A simple medium for rapid regeneration of Bacillus subtilis protoplasts transformed with plasmid DNA. FEMS Microbiol Lett 40:1–5
Quick M, Wright EM (2002) Employing Escherichia coli to functionally express, purify, and characterize a human transporter. Proc Natl Acad Sci USA 99:8597–8601
Ritz D, Lim J, Reynolds CM, Poole LB, Beckwith J (2001) Conversion of a peroxiredoxin into a disulfide reductase by a triplet repeat expansion. Science 294:158–160
Rosenberg HF (1998) Isolation of recombinant secretory proteins by limited induction and quantitative harvest. Biotechniques 24:188–191
Rygus T, Hillen W (1991) Inducible high-level expression of heterologous genes in Bacillus megaterium using the regulatory elements of xylose-utilization operon. Appl Microbiol Biotechnol 35:594–599
Rygus T, Scheler A, Allmansberger R, Hillen W (1991) Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization. Arch Microbiol 155:535–542
Sagiya Y, Yamagata H, Udaka S (1994) Direct high-level secretion into the culture medium of tuna growth hormone in a biologically active form by Bacillus brevis. Appl Microbiol Biotechnol 42:358–363
Schmidt FR (2004) Recombinant expression systems in the pharmaceutical industry. Appl Microbiol Biotechnol 65:363–372
Schneider JC, Jennings AF, Mun DM, McGovern PM, Chew LC (2005) Auxotrophic markers pyrF and proC can replace antibiotic markers on protein production plasmids in high-cell-density Pseudomonas fluorescens fermentation. Biotechnol Prog 21:343–348
Shiga Y, Maki M, Ohta T, Tokishita S, Okamoto A, Tsukagoshi N, Udaka S, Konishi A, Kodama Y, Ejima D, Matsui H, Yamagata H (2000) Efficient production of N-terminally truncated biologically active human interleukin-6 by Bacillus brevis. Biosci Biotechnol Biochem 64:665–669
Shokri A, Sande’n AM, Larsson G (2003) Cell and process design for targeting of recombinant protein into the culture medium of Escherichia coli. Appl Microbiol Biotechnol 60:654–664
Singh SM, Panda AK (2005) Solubilization and refolding of bacterial inclusion body proteins. J Biosci Bioeng 99:303–310
Skerra A (1994) Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli. Gene 151:131–135
Stahl ML, Ferrari E (1984) Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro derived deletion mutation. J Bacteriol 158:411–418
Studier FW (1991) Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system. J Mol Biol 219:37–44
Studier FW, Moffatt BA (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189:113–130
Takimura Y, Kato M, Ohta T, Yamagata H, Udaka S (1997) Secretion of human interleukin-2 in biologically active form by Bacillus brevis directly into culture medium. Biosci Biotechnol Biochem 61:1858–1861
Tjalsma H, Bolhuis A, Jongbloed JD, van Dijl JM (2000) Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome. Microbiol Mol Biol Rev 64:515–547
Udaka S, Yamagata H (1993) High-Level secretion of heterologous proteins by Bacillus brevis. Methods Enzymol 217:23–33
Ulmanen K, Lundstrom P, Lehtovaara P, Sarvas M, Ruohonen M, Palva I (1985) Transcription and translation of foreign genes in Bacillus subtilis by the aid of a secretion vector. J Bacteriol 162:176–182
Umelo-Njaka E, Nomellini JF, Yim H, Smit J (2001a) Development of small high-copy-number plasmid vectors for gene expression in Caulobacter crescentus. Plasmid 46:37–46
Umelo-Njaka E, Nomellini JF, Bingle WH, Glasier LG, Irvin RT, Smit J (2001b) Expression and testing of Pseudomonasaeruginosa vaccine candidate proteins prepared with the Caulobactercrescentus S-layer protein expression system. Vaccine 19:1406–14015
Vorobjeva IP, Khmel LA, Alfödi L (1980) Transformation of Bacillus megaterium protoplasts by plasmid DNA. FEMS Microbiol Lett 7:261–263
Wang RF, Kushner SR (1991) Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene 100:195–199
Wang B, Yang X, Wu R (1993) High-level production of the mouse epidermal growth factor in a Bacillus brevis expression system. Protein Expr Purif 4:223–231
Westers L, Westers H, Quax WJ (2004) Bacillus subtilis as a cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism. Biochim Biophys Acta 1694:299–310
Wilms B, Hauck A, Reuss M, Syldatk C, Mattes R, Siemann M, Altenbuchner J (2001) High-cell-density fermentation for production of l-N-Carbamoylase using an expression system based on the Escherichia colirhaBAD promoter. Biotechnol Bioeng 73:95–103
Wong S-L, Kawamura F, Doi RH (1986) Use of the Bacillus subtilis subtilisin signal peptide for efficient secretion of TEM β-lactamase during growth. J Bacteriol 168:1005–1009
Wu S-C, Wong S-L (2002) Engineering of a Bacillus subtilis strain with adjustable levels of intracellular biotin for secretory production of functional streptavidin. Appl Environ Microbiol 68:1102–1108
Wu S-C, Wong S-L (2005) Engineering soluble monomeric streptavidin with reversible biotin binding capability. J Biol Chem 280:23225–23231
Wu X-C, Lee W, Tran L, Wong S-L (1991) Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases. J Bacteriol 173:4952–4958
Wu S-C, Yeung JC, Duan Y, Ye R, Szarka SJ, Habibi HR, Wong SL (2002) Functional production and characterization of a fibrin-specific single-chain antibody fragment from Bacillus subtilis: effects of molecular chaperones and a wall-bound protease on antibody fragment production. Appl Environ Microbiol 68:3261–3269
Yamagata H, Nakahama K, Suzuki Y, Kakinuma A, Tsukagoshi N, Udaka S (1989) Use of Bacillus brevis for efficient synthesis and secretion of human epidermal growth factor. Proc Natl Acad Sci USA 86:3589–3593
Yang S, Huang H, Zhang R, Huang X, Li S, Yuan Z (2001) Expression and purification of extracellular penicillin G acylase in Bacillus subtilis. Protein Expr Purif 21:60–64