Sử dụng RNAi đường miệng để kiểm soát Drosophila suzukii: thử nghiệm trong phòng thí nghiệm đối với giai đoạn ấu trùng và trưởng thành

Springer Science and Business Media LLC - Tập 89 - Trang 803-814 - 2016
Clauvis Nji Tizi Taning1,2, Olivier Christiaens1, Nick Berkvens2, Hans Casteels2, Martine Maes2, Guy Smagghe1
1Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
2Plant Science Unit - Crop Protection, Institute for Agricultural and Fisheries Research, Merelbeke, Belgium

Tóm tắt

Drosophila có cánh đốm (Drosophila suzukii) là một loài sâu hại xâm lấn và gây thiệt hại kinh tế nghiêm trọng cho các loại trái cây nhỏ và trái cây hạch. Việc kiểm soát chúng là rất khó khăn. Can thiệp RNA (RNAi) hay tắt gen qua RNA đôi sợi (dsRNA) đang nhanh chóng trở thành một công cụ thuộc về sinh học chức năng thường được sử dụng trong côn trùng và có tiềm năng lớn cho việc kiểm soát sâu hại. Nghiên cứu này điều tra xem liệu RNAi có hoạt động trong D. suzukii hay không và liệu việc cung cấp dsRNA qua đường miệng có thể kích hoạt sự tắt gen và hoạt động diệt côn trùng hay không. Đầu tiên, việc tiêm dsRNA nhắm mục tiêu vào hai gen thiết yếu (alpha COP và shrb) vào huyết tương của ruồi trưởng thành đã được thực hiện, xác nhận rằng hệ thống RNAi là hoạt động và sự tắt gen dẫn đến tỷ lệ chết. Thứ hai, dsRNA nhắm tới alpha-COP và hai gen thiết yếu bổ sung, rpl13 và vha26, đã được trộn với chế độ ăn nhân tạo và cho ăn cho các giai đoạn ấu trùng và trưởng thành của D. suzukii. Với dsRNA trần, không có sự tắt gen rõ ràng và tỷ lệ chết được ghi nhận. Tuy nhiên, việc kết hợp dsRNA với chất tái tổ hợp đã dẫn đến sự tăng đáng kể trong tắt gen và tỷ lệ chết côn trùng. Kết quả tốt nhất đạt được với ds-Vha26. Các kết quả được thảo luận liên quan đến việc tối ưu hóa trong tương lai về cách sản xuất, công thức, sự kết hợp và cung cấp dsRNA.

Từ khóa

#Drosophila suzukii #RNAi #dsRNA #kiểm soát sâu hại #tắt gen

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