Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Sử dụng RNAi đường miệng để kiểm soát Drosophila suzukii: thử nghiệm trong phòng thí nghiệm đối với giai đoạn ấu trùng và trưởng thành
Tóm tắt
Drosophila có cánh đốm (Drosophila suzukii) là một loài sâu hại xâm lấn và gây thiệt hại kinh tế nghiêm trọng cho các loại trái cây nhỏ và trái cây hạch. Việc kiểm soát chúng là rất khó khăn. Can thiệp RNA (RNAi) hay tắt gen qua RNA đôi sợi (dsRNA) đang nhanh chóng trở thành một công cụ thuộc về sinh học chức năng thường được sử dụng trong côn trùng và có tiềm năng lớn cho việc kiểm soát sâu hại. Nghiên cứu này điều tra xem liệu RNAi có hoạt động trong D. suzukii hay không và liệu việc cung cấp dsRNA qua đường miệng có thể kích hoạt sự tắt gen và hoạt động diệt côn trùng hay không. Đầu tiên, việc tiêm dsRNA nhắm mục tiêu vào hai gen thiết yếu (alpha COP và shrb) vào huyết tương của ruồi trưởng thành đã được thực hiện, xác nhận rằng hệ thống RNAi là hoạt động và sự tắt gen dẫn đến tỷ lệ chết. Thứ hai, dsRNA nhắm tới alpha-COP và hai gen thiết yếu bổ sung, rpl13 và vha26, đã được trộn với chế độ ăn nhân tạo và cho ăn cho các giai đoạn ấu trùng và trưởng thành của D. suzukii. Với dsRNA trần, không có sự tắt gen rõ ràng và tỷ lệ chết được ghi nhận. Tuy nhiên, việc kết hợp dsRNA với chất tái tổ hợp đã dẫn đến sự tăng đáng kể trong tắt gen và tỷ lệ chết côn trùng. Kết quả tốt nhất đạt được với ds-Vha26. Các kết quả được thảo luận liên quan đến việc tối ưu hóa trong tương lai về cách sản xuất, công thức, sự kết hợp và cung cấp dsRNA.
Từ khóa
#Drosophila suzukii #RNAi #dsRNA #kiểm soát sâu hại #tắt genTài liệu tham khảo
Allen ML, Walker WB (2012) Saliva of Lygus lineolaris digests double stranded ribonucleic acids. J Insect Physiol 58(3):391–396
Amdam GV, Simoes ZL, Guidugli KR, Norberg K, Omholt SW (2003) Disruption of vitellogenin gene function in adult honeybees by intra-abdominal injection of double-stranded RNA. BMC Biotechnol 3:1
Babst M, Katzmann DJ, Estepa-Sabal EJ, Meerloo T, Emr SD (2002) Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev Cell 3(2):271–282
Baum JA, Bogaert T, Clinton W, Heck GR, Feldmann P, Ilagan O, Johnson S, Plaetinck G, Munyikwa T, Pleau M (2007) Control of coleopteran insect pests through RNA interference. Nat Biotechnol 25:1322–1326
Bautista MAM, Miyata T, Miura K, Tanaka T (2009) RNA interference-mediated knockdown of a cytochrome P450, CYP6BG1, from the diamondback moth, Plutella xylostella, reduces larval resistance to permethrin. Insect Biochem Mol Biol 39:38–46
Beers EH, Smith TJ, Walsh D (2010) Spotted wing drosophila. Washington State University Tree Fruit Research and Extension Center: Orchard Pest Management
Bolognesi R, Ramaseshadri P, Anderson J, Bachman P, Clinton W, Flannagan R (2012) Characterizing the mechanism of action of double-stranded RNA activity against western corn rootworm (Diabrotica virgifera virgifera LeConte). PLoS ONE 7(10):e47534
Calabria G, Máca J, Bächli G, Serra L, Pascual M (2012) First records of the potential pest species Drosophila suzukii (Diptera: Drosophilidae) in Europe. J Appl Entomol 136(1–2):139–147
Chabert S, Allemand R, Poyet M, Eslin P, Gibert P (2012) Ability of European parasitoids (Hymenoptera) to control a new invasive Asiatic pest, Drosophila suzukii. Biol Control 63(1):40–47
Chiu JC, Jiang X, Zhao L, Hamm CA, Cridland JM, Saelao P et al (2013) Genome of Drosophila suzukii, the spotted wing drosophila. Genes Genomes Genet G3:g3–g113
Christiaens O, Swevers L, Smagghe G (2014) DsRNA degradation in the pea aphid (Acyrthosiphon pisum) associated with lack of response in RNAi feeding and injection assay. Peptides 53:307–314
Dong Y, Friedrich M (2005) Nymphal RNAi: systemic RNAi mediated gene knockdown in juvenile grasshopper. BMC Biotechnol 5:25
Dreves AJ, Walton VM, Fisher GC (2009) A new pest attacking healthy ripening fruit in Oregon: spotted wing Drosophila: Drosophila suzukii (Matsumura). Extension Service, Oregon State University, Corvallis
Evans PD, Cook CN, Riggs PD, Noren CJ (1995) LITMUS; multipurpose cloning vectors with a novel system for bidirectional in vitro transcription. Biotechniques 19:130–135
Fire A, Xu SQ, Montgomery MK, Kostas SA, Driver SE, Mello CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806–811
Gerich B, Orci L, Tschochner H, Lottspeich F, Ravazzola M, Amherdt M, Harter C (1995) Non-clathrin-coat protein alpha is a conserved subunit of coatomer and in Saccharomyces cerevisiae is essential for growth. Proc Natl Acad Sci 92(8):3229–3233
Goodhue RE, Bolda M, Farnsworth D, Williams JC, Zalom FG (2011) Spotted wing drosophila infestation of California strawberries and raspberries: economic analysis of potential revenue losses and control costs. Pest Manag Sci 67:1396–1402
Gordon KHJ, Waterhouse PM (2007) RNAi for insect-proof plants. Nat Biotechnol 25:1231–1232
Hannon GJ, Rossi JJ (2004) Unlocking the potential of the human genome with RNA interference. Nature 431:371–378
Hunter WB, Glick E, Paldi N, Bextine BR (2012) Advances in RNA interference: dsRNA treatment in trees and grapevines for insect pest suppression. Southwest Entomol 37(1):85–87
Huvenne H, Smagghe G (2010) Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review. J Insect Physiol 56(3):227–235
Jaubert-Possamai S, Trionnaire G Le, Bonhomme J, Christophides GK, Rispe C, Tagu D (2007) Gene knockdown by RNAi in the pea aphid Acyrthosiphon pisum. BMC Biotechnol 7:63
Jin S, Singh ND, Li L, Zhang X, Daniell H (2015) Engineered chloroplast dsRNA silences cytochrome p450 monooxygenase, V-ATPase and chitin synthase genes in the insect gut and disrupts Helicoverpa armigera larval development and pupation. Plant Biotechnol J 13(3):435–446
Kamath RS, Ahringer J (2003) Genome-wide RNAi screening in Caenorhabditis elegans. Methods 30:313–321
Kamath RS, Fraser AG, Dong Y, Poulin G, Durbin R, Gotta M (2003) Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 42:231–237
Kanzawa T (1939) Studies on Drosophila suzukii Mats. Stud Drosophila suzukii Mats
Kim VN, Han J, Siomi MC (2009) Biogenesis of small RNAs in animals. Nat Rev Mol Cell Biol 10(2):126–139
Koči J, Ramaseshadri P, Bolognesi R, Segers G, Flannagan R, Park Y (2014) Ultrastructural changes caused by Snf7 RNAi in larval enterocytes of western corn rootworm (Diabrotica virgifera virgifera Le Conte). PLoS ONE 9(1):e83985
Kumar P, Pandit SS, Baldwin IT (2012) Tobacco rattle virus vector: a rapid and transient means of silencing Manduca sexta genes by plant mediated RNA interference. PLoS ONE 7:e31347
Landolt PJ, Adams T, Rogg H (2012) Trapping spotted wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), with combinations of vinegar and wine, and acetic acid and ethanol. J Appl Entomol 136(1–2):148–154
Lebreton S, Witzgall P, Olsson M, Becher PG (2014) Dietary glucose regulates yeast consumption in adult Drosophila males. Front Physiol 5:504
Lee JC, Bruck DJ, Dreves AJ, Ioriatti C, Vogt H, Baufeld P (2011) In focus: Spotted-wing drosophila, Drosophila suzukii, across perspectives. Pest Manag Sci 67(11):1349–1351
Li H, Guan R, Guo H, Miao X (2015) New insights into an RNAi approach for plant defence against piercing‐sucking and stem‐borer insect pests. Plant Cell Environ
Liu S, Ding Z, Zhang C, Yang B, Liu Z (2010) Gene knockdown by intro-thoracic injection of double-stranded RNA in the brown planthopper, Nilaparvata lugens. Insect Biochem Mol Biol 40:666–671
Liu J, Smagghe G, Swevers L (2013) Transcriptional response of BmToll9-1 and RNAi machinery genes to exogenous dsRNA in the midgut of Bombyx mori. J Insect Physiol 59(6):646–654
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25(4):402–408
Mao YB, Cai WJ, Wang JW, Hong GJ, Tao XY, Wang LJ, Huang YP, Chen XY (2007) Silencing a cotton bollworm P450 monooxygenase gene by plant mediated RNAi impairs larval tolerance to gossypol. Nat Biotechnol 25:1307–1313
Mitsui H, Takahashi KH, Kimura MT (2006) Spatial distributions and clutch sizes of Drosophila species ovipositing on cherry fruits of different stages. Popul Ecol 48(3):233–237
Price DRG, Gatehouse JA (2008) RNAi-mediated crop protection against insects. Trends Biotechnol 26:393–400
Rajagopal R, Sivakumar S, Agrawal N, Malhotra P, Bhatnagar RK (2002) Silencing of midgut aminopeptidase N of Spodoptera litura by double-stranded RNA establishes its role as Bacillus thuringiensis toxin receptor. J Biol Chem 277:46849–46851
Ramaseshadri P, Segers G, Flannagan R, Wiggins E, Clinton W, Ilagan O, Bolognesi R (2013) Physiological and cellular responses caused by RNAi- mediated suppression of Snf7 orthologue in western corn rootworm (Diabrotica virgifera virgifera) Larvae. PLoS ONE 8(1):e54270
Saleh MC, van Rij RP, Hekele A, Gillis A, Foley E, O’Farrell PH, Andino R (2006) The endocytic pathway mediates cell entry of dsRNA to induce RNAi silencing. Nat Cell Biol 8(8):793–802
Shakesby AJ, Wallace IS, Isaacs HV, Pritchard J, Roberts DM, Douglas AE (2009) A water-specific aquaporin involved in aphid osmoregulation. Insect Biochem Mol Biol 39:1–10
Singh AD, Wong S, Ryan CP, Whyard S (2013) Oral delivery of double-stranded RNA in larvae of the yellow fever mosquito, Aedes aegypti: implications for pest mosquito control. J Insect Sci 13(1):69
Timmons L, Court DL, Fire A (2001) Ingestion of bacteria expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 263:103–112
Tomoyasu Y, Miller SC, Tomita S, Schoppmeier M, Grossman D, Bucher G (2008) Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium. Genome Biol 9:R10
Ulrich J, Dao VA, Majumdar U, Schmitt-Engel C, Schwirz J, Schultheis D et al (2015) Large scale RNAi screen in Tribolium reveals novel target genes for pest control and the proteasome as prime target. BMC Genom 16(1):674
Walsh DB, Bolda MP, Goodhue RE, Dreves AJ et al (2011) Drosophila suzukii (Diptera: Drosophilidae): invasive pest of ripening soft fruit expanding its geographic range and damage potential. J Integr Pest Manag 2(1):G1–G7
Wang Z, Morris JC, Drew ME, Englund PT (2000) Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters. J Biol Chem 275(51):40174–40179
Whyard S, Singh AD, Wong S (2009) Ingested double-stranded RNAs can act as species specific insecticides. Insect Biochem Mol Biol 39:824–832
Wuriyanghan H, Falk BW (2013) RNA interference towards the potato psyllid, Bactericera cockerelli, is induced in plants infected with recombinant Tobacco mosaic virus (TMV). PLoS ONE 8(6):e66050
Wuriyanghan H, Rosa C, Falk BW (2011) Oral delivery of double-stranded RNAs and siRNAs induces RNAi effects in the potato/tomato psyllid, Bactericerca cockerelli. PLoS ONE 6(11):e27736
Wynant N, Santos D, Van Wielendaele P, Vanden Broeck J (2014) Scavenger receptor-mediated endocytosis facilitates RNA interference in the desert locust, Schistocerca gregaria. Insect Mol Biol 23(3):320–329
Yu N, Christiaens O, Liu J, Niu J, Cappelle K (2013) Delivery of dsRNA for RNAi in insects: an overview and future directions. Insect Sci 20:4–14
Zha W, Peng X, Chen R, Du B, Zhu L (2011) Knockdown of midgut genes by dsRNA-transgenic plant-mediated RNA interference in the hemipteran insect Nilaparvata lugens. PLoS ONE 6:e20504
Zhang X, Zhang J, Zhu KY (2010) Chitosan/double-stranded RNA nanoparticle mediated RNA interference to silence chitin synthase genes through larval feeding in the African malaria mosquito (Anopheles gambiae). Insect Mol Bio 19:683–693
Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R (2015) Full crop protection from an insect pest by expression of long double-stranded RNAs in plastids. Science 347(6225):991–994
Zhou Y, Ching YP, Kok KH, Kung HF, Jin DY (2002) Post-transcriptional suppression of gene expression in Xenopus embryos by small interfering RNA. Nucleic Acids Res 30(7):1664–1669
Zhou X, Wheeler MM, Oi FM, Shcharf ME (2008) RNA interference in the termite Reticulitermes flavipes through ingestion of double-stranded RNA. Insect Biochem Mol Biol 38:805–815
Zhu J, Park KC, Baker TC (2003) Identification of odors from overripe mango that attract vinegar flies, Drosophila melanogaster. J Chem Ecol 29:899–909
Zhu F, Xu J, Palli R, Ferguson J, Palli SR (2011) Ingested RNA interference for managing the populations of the Colorado potato beetle Leptinotarsa decemlineata. Pest Manag Sci 67(2):175–182
