Oligomeric forms of potato spindle tuber viroid (PSTV) and of its complementary RNA are present in nuclei isolated from viroid-infected potato cells

Bioscience Reports - Tập 3 Số 8 - Trang 767-774 - 1983
Ellen Spiesmacher1, Hans‐Peter Mühlbach1, Martina Schnölzer1, Bernd Haas1, Heinz L. Sänger1
1Abteilung Viroidforschung, Max-Planck-Institut fur Biochemie, D-8033 Planegg-Martinsried, Federal Republic of Germany

Tóm tắt

Different oligomeric forms of PSTV are detected in nuclei isolated from PSTV-infected potato cells by means of molecular hybridization, using as probes synthetic oligodeoxyribonucleotides with sequence specificity for (+)PSTV and for (−)PSTV. In addition to several species of longer-than-unit-length (−)PSTV molecules, two oligomeric forms os (+)PSTV are detected, which correspond in size to RNA strands of approximately two and three times viroid unit-length. They must be considered as the precursors os the circular and linear (+)PSTV monomers accumulating in the cell nucleus.

Từ khóa


Tài liệu tham khảo

Sänger HL, Klotz G, Riesner D, Gross HJ & Kleinschmidt AK (1976) Proc. Natl. Acad. Sci. U.S.A.73, 3852?3856.

Gross HJ, Domdey H, Lossow C, Jank P, Raba M, Alberty H & Sänger HL (1978) Nature (London)273, 203?208.

Riesner D, Henco K, Rokohl U, Klotz G, Kleinschmidt AK, Domdey H, Jank P, Gross HJ & Sänger HL (1979) J. Mol. Biol.133, 85?115.

Sänger HL (1982) in Encyclopedia of Plant Physiology, New Series, vol 14B, Nucleic Acids and Proteins in Plants II (Parthier B & Boulter D, eds), pp 368?454, Springer, Berlin, Heidelberg, New York.

Mühlbach HP & Sänger HL (1979) Nature (London)278, 185?188.

Rackwitz HR, Rohde W & Sänger HL (1981) Nature (London)291, 297?301.

Branch A, Robertson HD, Greer CH, Gegenheimer P, Peebles C & Abelson J (1982) Science217, 1147?1149.

Kikuchi Y, Tyc K, Filipowicz W, Sänger HL & Gross HJ (1982) Nucleic Acids Res.10, 7521?7529.

Sänger HL (1972) Adv. Biosci.8, 103?116.

Diener TO (1971) Virology43, 75?89.

Grill LK & Semancik JS (1978) Proc. Natl. Acad. Sci. U.S.A.75, 896?900.

Rohde W & Sänger HL (1981) Biosci. Rep.1, 327?336.

Branch AD, Robertson HD & Dickson EC (1981) Proc. Natl. Acad. Sci. U.S.A.78, 6381?6385.

Owens RA & Diener TO (1982) Proc. Natl. Acad. Sci. U.S.A.79, 113?117.

Zelcer A, Zaitlin M, Robertson HD & Dickson E (1982) J. Gen. Virol.59, 139?148.

Mühlbach HP, Faustmann O & Sänger HL (submitted to Plant Molec. Biol.)

Takahashi T & Diener TO (1975) Virology64, 106?114.

Flores R & Semancik JS (1983) Proc. Natl. Acad. Sci. U.S.A.79, 6285?6288.

Willmitzer L & Wagner KG (1981) Exp. Cell Res.5, 69?77.

Sänger HL & Ramm K (1975) in Modification of the information Content of Plant Cells (Markam R, Davies DR, Hopwood DA & Horne RW, eds), pp 229?252, North Holland, Amsterdam.

McMaster GK & Carmichael GG (1977) Proc. Natl. Acad. Sci. U.S.A.74, 4835?4838.

Stellwag EJ & Dahlberg AE (1980) Nucleic Acids Res.8, 299?317.

Matteuci MD & Caruthers MH (1981) J. Amer. Chem. Soc.103, 3185?3191.

Beaucage SL & Caruthers M (1981) Tetrahedron Lett.22, 1859?1862.

Chow F, Kempe T & Palm G (1981) Nucleic Acids Res.9, 2807?2817.

Joy E, Bambara RA, Padmanabhan R & Wu R (1974) Nucleic Acids Res.1, 331?353.

Semancik JS & Desjardins PR (1980) Virology104, 117?121.

Bruening G, Gould AR, Murphy PJ & Symons RH (1982) FEBS Lett.148, 71?78.

Haseloff J, Mohamed NA & Symons RH (1982) Nature (London)299, 316?321.

Thông tin
Thông tin xuất bản

Bioscience Reports

Tập 3 Số 8

767-774

Thông tin tác giả