Nucleotide sequence of a cDNA coding for mitochondrial fumarase from human liver

Bioscience Reports - Tập 6 Số 10 - Trang 921-929 - 1986
B. Therese Kinsella1, Shawn Doonan1
1Department of Biochemistry University College, Cork, Ireland

Tóm tắt

The nucleotide sequence of a 1.46 kb cDNA, selected from a human liver library by the expression of fumarase antigenic determinants, was determined using the dideoxy chain termination method. The cDNA contained an open reading frame extending from the extreme 5′-base and coding for a protein with 468 amino acids. This protein, with the exception of an N-terminal methionine, was identified as mitochondrial fumarase. The protein showed a high degree of identity of structure with the fumarase from Bacillus subtilis (56.6 %) and a fumarase from Escherichia coli (product of the fumC gene, 59.3 %), and a lower degree of identity with the aspartase of E. coli (37.2 %).

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Bioscience Reports

Tập 6 Số 10

921-929

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