Not the one, but the only one: about Cannabis cryptic virus in plants showing ‘hemp streak’ disease symptoms

Springer Science and Business Media LLC - Tập 150 - Trang 575-588 - 2017
Laura Righetti1, Roberta Paris1, Claudio Ratti2, Matteo Calassanzio2, Chiara Onofri1, Davide Calzolari1,3, Wulf Menzel4, Dennis Knierim5, Gianmaria Magagnini1, Daniela Pacifico1, Gianpaolo Grassi1
1CREA - Council for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops, Bologna, Italy
2DipSA - Plant Pathology, University of Bologna, Bologna, Italy
3Department of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Piacenza, Italy
4Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany
5Leibniz Institute, DSMZ – German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany

Tóm tắt

Interveinal chlorosis and leaf margin wrinkling are widespread symptoms of Cannabis sativa. They are traditionally attributed to the so-called hemp streak virus (HSV), but its existence has not been demonstrated yet. To our knowledge, no molecular investigation has so far been performed in order to identify the causal agent of this symptomatology, we therefore decided to use traditional and molecular virology techniques to better characterize symptoms and pursue the etiological agent. No pathogenic virus was found by using targeted PCR reactions and by RNA sequencing, whereas we were able to detect the Cannabis cryptic virus (CanCV) with both techniques. We, therefore, developed an RT-qPCR assay based on a CanCV-specific TaqMan probe and applied it to a wide range of symptomatic and symptomless plants, using a two-step (for quantification), or a one-step (for fast detection) protocol. Both symptoms and the virus were only shown to be transmitted vertically and did not pass via mechanical inoculation or grafting, though we could not find any cause-effect correlation between them. In fact, the virus was found in all the tested hemp samples, and its abundance varied greatly between different accessions and individuals, independently from the presence and severity of symptoms. The suggestion that hemp streak is caused by a virus is therefore questioned. Some abiotic stresses seem to play a role in triggering the symptoms but this aspect needs further investigation. For breeding purposes, a selection of parental plants based on the absence of symptoms proved to be efficient in containment of the disease.

Tài liệu tham khảo

Adams, I. P., Miano, D. W., Kinyua, Z. M., Wangai, A., Kimani, E., Phiri, N., et al. (2013). Use of next-generation sequencing for the identification and characterization of maize chlorotic mottle virus and sugarcane mosaic virus causing maize lethal necrosis in kenya. Plant Pathology, 62(4), 741–749. doi:10.1111/j.1365-3059.2012.02690.x.

Badge, J., Brunt, A., Carson, R., Dagless, E., Karamagioli, M., Phillips, S., et al. (1996). A carlavirus-specific PCR primer and partial nucleotide sequence provides further evidence for the recognition of cowpea mild mottle virus as a whitefly-transmitted carlavirus. European Journal of Plant Pathology, 102(3), 305–310. doi:10.1007/BF01877970.

Chen, J., Chen, J., & Adams, M. J. (2001). A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family. Archives of Virology, 146(4), 757–766. doi:10.1007/s007050170144.

Chen, L., Chen, J. S., Liu, L., Yu, X., Yu, S., Fu, T. Z., & Liu, W. H. (2006). Complete nucleotide sequences and genome characterization of double-stranded RNA 1 and RNA 2 in the Raphanus sativus-root cv. Yipinghong. Archives of Virology, 151(5), 849–859. doi:10.1007/s00705-005-0685-8.

Chiba, S., Kondo, H., Tani, A., Saisho, D., Sakamoto, W., Kanematsu, S., & Suzuki, N. (2011). Widespread endogenization of genome sequences of non-retroviral rna viruses into plant genomes. PLoS Pathogens, 7(7), e1002146. doi:10.1371/journal.ppat.1002146.

Chomič, A., Pearson, M. N., Clover, G. R. G., Farreyrol, K., Saul, D., Hampton, J. G., & Armstrong, K. F. (2010). A generic RT-PCR assay for the detection of Luteoviridae. Plant Pathology, 59(3), 429–442. doi:10.1111/j.1365-3059.2010.02282.x.

Clover, G. R. G., Pearson, M. N., Elliott, D. R., Tang, Z., Smales, T. E., & Alexander, B. J. R. (2003). Characterization of a strain of Apple stem grooving virus in Actinidia chinensis from China. Plant Pathology, 52(3), 371–378. doi:10.1046/j.1365-3059.2003.00857.x.

Digiaro, M., Elbeaino, T., & Martelli, G. P. (2007). Development of degenerate and species-specific primers for the differential and simultaneous RT-PCR detection of grapevine-infecting nepoviruses of subgroups A, B and C. Journal of Virological Methods, 141(1), 34–40. doi:10.1016/j.jviromet.2006.11.033.

Ferri, F. (1963). Alterazioni della canapa trasmesse per seme. Progresso Agricolo, 9, 346–351.

Foissac, X., Svanella-Dumas, L., Gentit, P., Dulucq, M.-J., Marais, A., & Candresse, T. (2005). Polyvalent degenerate oligonucleotides reverse transcription-polymerase chain reaction: a polyvalent detection and characterization tool for trichoviruses, capilloviruses, and foveaviruses. Phytopathology, 95(6), 617–625. doi:10.1094/PHYTO-95-0617.

Fredricks, D. N., & Relman, D. A. (1996). Sequence-based identification of microbial pathogens: A reconsideration of Koch’s postulates. Clinical Microbiology Reviews, 9(1), 18–33.

Froussard, P. (1992). A random-PCR method (rPCR) to construct whole cDNA library from low amounts of RNA. Nucleic Acids Research, 20(11), 2900.

Hoagland, D. R., & Arnon, D. I. (1950). The water culture method for growing plants without soil. Barkley: The college of Agriculture, University of California Circular, 347.

Hyder, R., Pennanen, T., Hamberg, L., Vainio, E. J., Piri, T., & Hantula, J. (2013). Two viruses of Heterobasidion confer beneficial, cryptic or detrimental effects to their hosts in different situations. Fungal Ecology, 6, 387–396. doi:10.1016/j.funeco.2013.05.005.

Koziel, S. P. (2010). Genetic analysis of lignification and secondary wall development in bast fibers of industrial hemp (Cannabis sativa). Master’s Thesis in Plant Biology. University of Alberta. Retrieved from https://era.library.ualberta.ca/files/tt44pn501#.VkDHsNIvdpg.

Kusari, P., Kusari, S., Spiteller, M., & Kayser, O. (2013). Endophytic fungi harbored in Cannabis sativa L.: diversity and potential as biocontrol agents against host plant-specific phytopathogens. Fungal Diversity, 60(1), 137–151. doi:10.1007/s13225-012-0216-3.

Lamichhane, J. R., & Venturi, V. (2015). Synergisms between microbial pathogens in plant disease complexes: a growing trend. Frontiers in Plant Science, 6(May), 1–12. doi:10.3389/fpls.2015.00385.

Letschert, B., Adam, G., Lesemann, D. E., Willingmann, P., & Heinze, C. (2002). Detection and differentiation of serologically cross-reacting tobamoviruses of economical importance by RT-PCR and RT-PCR-RFLP. Journal of Virological Methods, 106(1), 1–10. doi:10.1016/S0166-0934(02)00135-0.

Lyttle, D. J., Orlovich, D. a., & Guy, P. L. (2011). Detection and analysis of endogenous badnaviruses in the New Zealand flora. AoB PLANTS, 11(1), 1–13. doi:10.1093/aobpla/plr008.

Marks, M. D., Tian, L., Wenger, J. P., Omburo, S. N., Soto-Fuentes, W., He, J., et al. (2009). Identification of candidate genes affecting Delta9-tetrahydrocannabinol biosynthesis in Cannabis sativa. Journal of Experimental Botany, 60(13), 3715–3726. doi:10.1093/jxb/erp210.

McPartland, J. M. (1996). A review of Cannabis diseases. Journal of the International Hemp Association, 3(9), 19–23 http://www.internationalhempassociation.org/jiha/iha03111.html.

McPartland, J. M., Clarke, R. C., & Watson, D. P. (2000). Hemp diseases and pests: management and biological control. New York: CABI Publishing.

Minguzzi, S., Terlizzi, F., Lanzoni, C., Poggi Pollini, C., & Ratti, C. (2016). A rapid protocol of crude RNA/DNA extraction for RT-qPCR detection and quantification of ‘Candidatus phytoplasma prunorum’. PLoS ONE, 11(1), e0146515. doi:10.1371/journal.pone.0146515.

Mohan Ram, H. Y., & Sett, R. (1982). Induction of fertile male flowers in genetically female Cannabis sativa plants by silver nitrate and silver thiosulphate anionic complex. Theoretical and Applied Genetics, 62(4), 369–375. doi:10.1007/BF00275107.

Nibert, M. L., Ghabrial, S. A., Maiss, E., Lesker, T., Vainio, E. J., Jiang, D., & Suzuki, N. (2014). Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research. Virus Research, 188, 128–141. doi:10.1016/j.virusres.2014.04.007.

Olmos, A., Bertolini, E., Gil, M., & Cambra, M. (2005). Real-time assay for quantitative detection of non-persistently transmitted Plum pox virus RNA targets in single aphids. Journal of Virological Methods, 128, 151–155.

Pappu, H. R., & Druffel, K. L. (2009). Use of conserved genomic regions and degenerate primers in a PCR-based assay for the detection of members of the genus Caulimovirus. Journal of Virological Methods, 157(1), 102–104. doi:10.1016/j.jviromet.2008.11.014.

Potgieter, C. A., Castillo, A., Castro, M., Cottet, L., & Morales, A. (2013). A wild-type Botrytis cinerea strain co-infected by double-stranded RNA mycoviruses presents hypovirulence-associated traits. Virology Journal, 10(1), 220. doi:10.1186/1743-422X-10-220.

Röder, K. (1941). Einige Untersuchungen über ein an Hanf (Cannabis sativa L.) auftretendes Virus. Faserforschung, 15, 77–81.

Roossinck, M. J. (2011). The good viruses: viral mutualistic symbioses. Nature reviews. Microbiology, 9(2), 99–108. doi:10.1038/nrmicro2491.

Roossinck, M. J. (2015). Move over bacteria! Viruses make their mark as mutualistic microbial symbionts. Journal of Virology, 89, 6532–6535. doi:10.1128/JVI.02974-14.

Turina, M., Ricker, M. D., Lenzi, R., Masenga, V., & Ciuffo, M. (2007). A severe disease of tomato in the Culiacan area (Sinaloa, Mexico) is caused by a new picorna-like viral species. Plant Disease, 91(8), 932–941. doi:10.1094/PDIS-91-8-0932.

Tzanetakis, I. E., & Martin, R. R. (2008). A new method for extraction of double-stranded RNA from plants. Journal of Virological Methods, 149(1), 167–170. doi:10.1016/j.jviromet.2008.01.014.

Tzanetakis, I. E., Price, R., & Martin, R. R. (2008). Nucleotide sequence of the tripartite Fragaria chiloensis cryptic virus and presence of the virus in the Americas. Virus Genes, 36(1), 267–272. doi:10.1007/s11262-007-0186-9.

van Bakel, H., Stout, J. M., Cote, A. G., Tallon, C. M., Sharpe, A. G., Hughes, T. R., & Page, J. E. (2011). The draft genome and transcriptome of Cannabis sativa. Genome Biology, 12(10), R102. doi:10.1186/gb-2011-12-10-r102.

van den Broeck, H. C., Maliepaard, C., Ebskamp, M. J. M., Toonen, M. A. J., & Koops, A. J. (2008). Differential expression of genes involved in C1 metabolism and lignin biosynthesis in wooden core and bast tissues of fibre hemp (Cannabis sativa L.) Plant Science, 174(2), 205–220. doi:10.1016/j.plantsci.2007.11.008.

Wei, T., & Clover, G. R. G. (2008). Use of primers with 5′ non-complementary sequences in RT-PCR for the detection of nepovirus subgroups A and B. Journal of Virological Methods, 153(1), 16–21. doi:10.1016/j.jviromet.2008.06.020.

Xiao, X., Cheng, J., Tang, J., Fu, Y., Jiang, D., Baker, T. S., et al. (2014). A novel partitivirus that confers hypovirulence on plant pathogenic fungi. Journal of Virology, 88(17), 10120–10133. doi:10.1128/JVI.01036-14.

Xie, W. S., Antoniw, J. F., White, R. F., & Jolliffe, T. H. (1994). Effects of beet cryptic virus infection on sugar beet in field trials. Annals of Applied Biology, 124(3), 451–459. doi:10.1111/j.1744-7348.1994.tb04150.x.

Ziegler, A., Matoušek, J., Steger, G., & Schubert, J. (2012). Complete sequence of a cryptic virus from hemp (Cannabis sativa). Archives of Virology, 157(2), 383–385. doi:10.1007/s00705-011-1168-8.

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