Use of primers with 5′ non-complementary sequences in RT-PCR for the detection of nepovirus subgroups A and B

Afonina, 2007, Primers with 5′ flaps improve real-time PCR, BioTechniques, 43, 770, 10.2144/000112631 Binladen, 2007, 5′-tailed sequencing primers improve sequencing quality of PCR products, BioTechniques, 42, 174, 10.2144/000112316 Bodrossy, 2003, Development and validation of a diagnostic microbial microarray for methanotrophs, Environ. Microbiol., 5, 566, 10.1046/j.1462-2920.2003.00450.x Card, 2007, Plant pathogens transmitted by pollen, Aust. Plant Path., 36, 455, 10.1071/AP07050 Digiaro, 2007, Development of degenerate and species-specific primers for the differential and simultaneous RT-PCR detection of grapevine-infecting nepoviruses of subgroups A, B and C, J. Virol. Meth., 141, 34, 10.1016/j.jviromet.2006.11.033 Dovas, 2003, A spot nested RT-PCR method for the simultaneous detection of members of the Vitivirus and Foveavirus genera in grapevine, J. Virol. Meth., 107, 99, 10.1016/S0166-0934(02)00197-0 Fauquet, 2005 Griesbach, 1995, Detection of tomato ringspot virus by polymerase chain reaction, Plant Dis., 79, 1054, 10.1094/PD-79-1054 Hall, T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98. Harrison, B.D.; Murant, A.F., 1977. Nepovirus group. CMI/AAB Descriptions of Plant Viruses No. 185. Jones, 2002, Improved PCR detection of Blackcurrant reversion virus in Ribes and further evidence that it is the causal agent of reversion disease, Plant Dis., 86, 1333, 10.1094/PDIS.2002.86.12.1333 Langeveld, 1991, Identification of potyviruses using the polymerase chain reaction with degenerate primers, J. Gen. Virol., 72, 1531, 10.1099/0022-1317-72-7-1531 Mackenzie, 1998, Ceratobium mosaic potyirus; another virus from orchids, Arch. Virol., 143, 1, 10.1007/s007050050341 Murant, A.F. 1970. Arabis mosaic virus. CMI/AAB Descriptions of Plant Viruses No. 16. Saldarelli, 1998, Use of degenerate primers in an RT-PCR assay for the identification and analysis of some filamentous viruses, with special reference to clostero- and vitiviruses of the grapevine, Eur. J. Plant Pathol., 104, 945, 10.1023/A:1008608506699 Sánchez-Navarro, 2005, Simultaneous detection and identification of eight stone fruit viruses by one-step RT-PCR, Eur. J. Plant Pathol., 111, 77, 10.1007/s10658-004-1422-y Wetzel, 2002, Simultaneous RT/PCR detection and differentiation of arabis mosaic and grapevine fanleaf nepoviruses in grapevines with a single pair of primers, J. Virol. Meth., 101, 63, 10.1016/S0166-0934(01)00422-0 Wilson, 2002, Sequence-specific identification of 18 pathogenic microorganisms using microarray technology, Mol. Cell. Probes, 16, 119, 10.1006/mcpr.2001.0397