New differences between isoenzymes A and B from Candida rugosa lipase

Biotechnology Letters - 1997
M.J. Herna´iz1, J.M. Sa´nchez-Montero1, J.V. Sinisterra1
1Department of Organic and Pharmaceutical Chemistry, Faculty of Pharmacy, Universidad Complutense, Madrid, Spain

Tóm tắt

Water adsorption isotherms of pure lipases A and B from Candida rugosa are different and can be used to distinguish between the isoenzymes. The maximum esterification yield (50%, 20h) can be achieved at initial 0.9<1.0. Lipase B is more stereoselective (49% yield, 98% enantiomeri excess) than lipase A (47% yield, 72% enantiomerci excess) but both isoenzymes mainly esterify the (S) 2(4-isobutylphenyl)propionic acid (Ibuprofen).

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Biotechnology Letters

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