Neuroprotective profile of pyruvate against ethanol-induced neurodegeneration in developing mice brain

Neurological Sciences - Tập 34 - Trang 2137-2143 - 2013
Najeeb Ullah1,2, Muhammad Imran Naseer1,3, Ikram Ullah1, Tae Hyun Kim1, Hae Young Lee1, Myeong Ok Kim1
1Division of Life Science, College of Natural Sciences (RINS) and Applied Life Science, Gyeongsang National University, Chinju, Republic of Korea
2Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan
3Center of Excellence in Genomic Medicine and Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia

Tóm tắt

Exposure to ethanol during developmental stages leads to several types of neurological disorders. Apoptotic neurodegeneration due to ethanol exposure is a main feature in alcoholism. Exposure of developing animals to alcohol induces apoptotic neuronal death and causes fetal alcohol syndrome. In the present study, we observed the possible protective effect of pyruvate against ethanol-induced neurodegeneration. Exposure of developing mice to ethanol (2.5 g/kg) induces apoptotic neurodegeneration and widespread neuronal cell death in the cortex and thalamus. Co-treatment of pyruvate (500 mg/kg) protects neuronal cell against ethanol by the reduced expression of caspase-3 in these brain regions. Immunohistochemical analysis and TUNNEL at 24 h showed that apoptotic cell death induced by ethanol in the cortex and thalamus is reduced by pyruvate. Histomorphological analysis at 24 h with cresyl violet staining also proved that pyruvate reduced the number of neuronal cell loss in the cortex and thalamus. The results showed that ethanol increased the expression of caspase-3 and thus induced apoptotic neurodegeneration in the developing mice cortex and thalamus, while co-treatment of pyruvate inhibits the induction of caspase-3 and reduced the cell death in these brain regions. These findings, therefore, showed that treatment of pyruvate inhibits ethanol-induced neuronal cell loss in the postnatal seven (P7) developing mice brain and may appear as a safe neuroprotectant for treating neurodegenerative disorders in newborns and infants.

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Neurological Sciences

Tập 34

2137-2143

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