Moving towards an integrated approach to molecular detection and identification ofToxoplasma gondii

Parasitology - Tập 137 Số 1 - Trang 1-11 - 2010
Chunlei Su1, E. Keats Shwab1, Pei Zhou2, Xingquan Zhu2, J. P. Dubey3
1Department of Microbiology, The University of Tennessee, Knoxville, TN 37996, USA
2Laboratory of Parasitology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
3Animal Parasitic Diseases Laboratory, United States Department of Agriculture, Beltsville, MD 20705, USA

Tóm tắt

SUMMARY

The development of simple, sensitive and rapid methods for the detection and identification ofToxoplasma gondiiis important for the diagnosis and epidemiological studies of the zoonotic disease toxoplasmosis. In the past 2 decades, molecular methods based on a variety of genetic markers have been developed, each with its advantages and limitations. The application of these methods has generated invaluable information to enhance our understanding of the epidemiology, population genetics and phylogeny ofT. gondii. However, since most studies focused solely on the detection but not genetic characterization ofT. gondii, the information obtained was limited. In this review, we discuss some widely used molecular methods and propose an integrated approach for the detection and identification ofT. gondii, in order to generate maximum information for epidemiological, population and phylogenetic studies of this key pathogen.

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Tài liệu tham khảo

10.1016/j.vetpar.2007.11.012

10.1016/S0020-7519(00)00124-7

10.1038/359082a0

10.1128/AAC.38.9.1930

10.1016/j.vetpar.2008.08.020

10.1016/j.ijpara.2007.09.004

10.1111/j.1574-6968.1998.tb13151.x

10.1590/S1807-59322009000300004

10.1016/j.meegid.2004.01.007

10.1046/j.1469-0691.2001.00224.x

10.1093/nar/gki604

10.3201/eid1206.060025

10.1073/pnas.0702356104

10.1016/j.diagmicrobio.2008.09.009

10.1128/JCM.39.6.2065-2071.2001

10.1016/S0020-7519(99)00170-8

10.1086/322800

Goldstein, 1999, Microsatellites: Evolution and Applications, 10.1093/oso/9780198504085.001.0001

10.1016/j.exppara.2007.08.006

10.1016/j.meegid.2004.12.004

10.1016/S0020-7519(00)00036-9

10.1111/j.1469-0691.2005.01332.x

10.1645/GE-1846.1

10.1016/j.vetpar.2008.07.036

10.1645/GE-1245.1

10.1016/j.ijpara.2007.11.012

10.1016/j.vetpar.2006.08.024

10.1016/j.vetpar.2007.03.009

10.1645/GE-1349.1

10.1016/j.vetpar.2007.03.008

10.1645/GE-1312.1

10.1016/j.vetpar.2006.12.001

Dubey, 1988, Toxoplasmosis of Animals and Man

10.1017/S0031182007003083

10.1099/0022-1317-51-10-879

Calderaro, 2006, Comparison between two real-time PCR assays and a nested-PCR for the detection of Toxoplasma gondii, Acta Bio Medica, 77, 75

Burg, 1989, Direct and sensitive detection of a pathogenic protozoan Toxoplasma gondii, by polymerase chain reaction, Journal of Clinical Microbiology, 27, 1787, 10.1128/JCM.27.8.1787-1792.1989

10.1645/0022-3395(2001)087[1472:HRTOTG]2.0.CO;2

Lin, 2000, Real-time PCR for quantitative detection of Toxoplasma gondii, Journal of Clinical Microbiology, 38, 4121, 10.1128/JCM.38.11.4121-4125.2000

10.1016/S1471-4922(02)02331-0

10.1111/j.1469-0691.2006.01642.x

10.1086/342663

10.1016/S0020-7519(01)00301-0

10.1016/j.ijpara.2004.06.007

10.1016/S0163-4453(94)90578-9

10.1016/j.vetpar.2004.09.005

10.2307/3283305

Su, 2009, Molecular Detection of Foodborne Pathogens, 741

10.1016/S0304-4017(01)00526-X

10.1016/j.ijpara.2007.11.010

10.1016/j.ijpara.2004.11.016

10.1016/S0140-6736(04)16412-X

10.1016/S0140-6736(04)16841-4

Howe, 1997, Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis, Journal of Clinical Microbiology, 35, 1411, 10.1128/JCM.35.6.1411-1414.1997

10.1128/JCM.43.4.1940-1943.2005

10.1111/j.1469-0691.2004.01073.x

10.1016/j.ijpara.2006.03.003

10.1093/infdis/172.6.1561

10.1590/S0074-02762009000200038

Khalifa, 1994, Value of PCR for evaluating occurrence of parasitemia in immunocompromised patients with cerebral and extracerebral toxoplasmosis, Journal of Clinical Microbiology, 32, 2813, 10.1128/JCM.32.11.2813-2819.1994

10.1073/pnas.0601438103

Costa, 2000, Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes, Journal of Clinical Microbiology, 38, 2929, 10.1128/JCM.38.8.2929-2932.2000

10.1590/S0074-02762009000200011

10.1128/JCM.43.12.5881-5887.2005

10.1016/j.vetpar.2008.04.021

Bretagne, 1995, Quantitative competitive PCR with bronchoalveolar lavage fluid for diagnosis of toxoplasmosis in AIDS patients, Journal of Clinical Microbiology, 33, 1662, 10.1128/JCM.33.6.1662-1664.1995

10.1645/GE-1409.1

10.1186/1471-2334-3-7

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Parasitology

Tập 137 Số 1

1-11

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