Mosquitoes of the Anopheles maculipennis group (Diptera: Culicidae) in Romania, with the discovery and formal recognition of a new species based on molecular and morphological evidence

Bulletin of Entomological Research - Tập 94 Số 6 - Trang 525-535 - 2004
Gabriela Nicolescu1, Yvonne-Marie Linton2, Alexandru Vladimirescu1, Theresa M. Howard2, Ralph E. Harbach2
1Department of Medical Entomology, Cantacuzino Institute, Bucharest, Romania.
2Mosquitoes Programme and Biomedical Sciences Theme, Department of Entomology, The Natural History Museum, Cromwell Road, London, SW7 5BD, UK

Tóm tắt

AbstractMosquitoes of the Anopheles maculipennis group were collected in five districts of Romania (Constanta, Giurgiu, Ilfov, Mehedinti and Suceava) between March 2000 and June 2003. Two hundred and ninety-seven specimens were identified by molecular methods. Nuclear rDNA ITS2 sequences of 178 specimens were compared with GenBank sequences for nine known Palaearctic species of the group, and 119 specimens were identified using an ITS2 PCR–RFLP assay developed during the study. Five genetically distinct species of the group were identified: A. atroparvus van Thiel, A. maculipennis Meigen, A. melanoon Hackett and A. messeae Falleroni and a previously unrecognized species. The new species, herein formally described and named A. daciaesp. n., was collected in the Black Sea coastal region and plains adjacent to the Danube River in southern Romania. Anopheles daciae is most similar to and sympatric with A. messeae. It is contrasted with A. messeae and characterized on the basis of unique nuclear ITS2 and mitochondrial COI DNA sequences and morphological characters of the eggs. The larval, pupal and adults stages of the two species were also compared, but no reliable characters were found to distinguish them. It seems likely that A. daciae is more widespread in eastern Europe and the Balkan States, and could be responsible for malaria transmission in these regions that is currently attributed to A. messeae. Anopheles melanoon is reported from Romania for the first time.

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Tài liệu tham khảo

Zotta, 1940, Nouvelle carte de la disribution des races d' Anopheles en Roumanie, Bulletin de la Section Scientifique de l'Académie Roumaine, 2, 73

10.1007/s004360050642

10.1007/BF02099755

Harbach R.E. & Knight K.L. (1980) Taxonomists' glossary of mosquito anatomy Plexus Publishing Inc., Marlton New Jersey.

10.1046/j.1365-2583.2002.00338.x

10.1603/0022-2585-39.3.446

Linton, 2001, New occurrence records for Anopheles maculipennis and An. messeae in northern Greece based on DNA sequence data, European Mosquito Bulletin, 11, 31

Nicolescu, 1996, George Zotta (1886–1942): an early concept of malaria stratification, Romanian Archives of Microbiology and Immunology, 2, 173

10.1603/0022-2585-41.4.582

10.1111/j.1365-2583.1996.tb00034.x

Falleroni, 1926, Fauna anofelica italiana e suo “habitat” (paludi, risaie, canali). Metodi di lotta contro la malaria, Rivista di Malariologia, 5, 553

Bruce-Chwatt, 1980, The rise and fall of malaria in Europe

Cianchi R. , Sabatini A. , Boccolini D. , Bullini L. , Coluzzi M. (1987) Electrophoretic evidence of reproductive isolation between sympatric populations of Anopheles melanoon and A. subalpinus. p. 1560 in Third International Congress on Malaria and Babesiosis International Laveran Foundation, Annecy France.

Gordeev, 2004, Intraspecific variability of the second internal transcribed spacer of the ribosomal DNA among five Palaearctic species of anopheline mosquitoes, European Mosquito Bulletin, 17, 14

10.1016/S0035-9203(34)90135-8

Hackett, 1935, A new variety of Anopheles maculipennis in southern Europe, Rivista di Malariologia, 14, 377

International Commission on Zoological Nomenclature. (1999) International code of zoological nomenclature 4th edn. The International Trust for Zoological Nomenclature London.

Jetten, 1994, Anophelism without malaria in Europe. A review of the ecology and distribution of the genus Anopheles in Europe, 94

Kitzmiller, 1967, Genetics of insect vectors of disease, 151

10.1093/bioinformatics/17.12.1244

Linton, 2002, Molecular confirmation of sympatric populations of Anopheles messeae and Anopheles atroparvus overwintering in Kent, southeast England, European Mosquito Bulletin, 13, 8

10.1046/j.1365-3113.2003.00198.x

10.1046/j.1365-2583.1999.00140.x

10.1046/j.1365-3113.2001.00153.x

Martini E. , Zotta G. (1934) Rapport sur un voyage d'études effectué à travers la Roumanie pendant les mois d'Août et de Septembre 1933. Races d' A. maculipennis en Roumanie. Societé des Nations, C.H. (Malaria) 218, 3–84.

Nikolaeva, 1996, Resurgence of malaria in the former Soviet Union (FSU), Society of Vector Ecology Newsletter, 27, 10

10.1093/aesa/87.6.651

10.1079/BER2003272

Stegnii, 1982, Recent developments in the genetics of insect vectors, 454

10.1093/nar/25.24.4876

White, 1978, Systematic reappraisal of the Anopheles maculipennis complex, Mosquito Systematics, 10, 13

Zotta, 1938, Contribution à l'étude de la distribution des races d' A. maculipennis en rapport avec les grandes lignes de repartition du paludisme en Roumanie, Archives Roumaines de Pathologie Expérimentale et de Microbiologie, 2, 209

Nicolescu, 1995, The mosquitoes of Romania: an annotated checklist and bibliography, Romanian Archives of Microbiology and Immunology, 54, 75

10.1046/j.1365-3113.2003.00211.x

Alten, 2000, Malaria and its vectors in Turkey, European Mosquito Bulletin, 7, 27

Frizzi, 1953, Etude cytogenetique d' Anopheles maculipennis en Italie, Bulletin of the World Health Organization, 9, 335

Boccolini, 2003, Further molecular and morphological support for the formal synonymy of Anopheles subalpinus Hackett and Lewis with A. melanoon Hackett, European Mosquito Bulletin, 16, 1

Linton, 2002, Observations on the taxonomic status of Anopheles subalpinus Hackett and Lewis and An. melanoon Hackett, European Mosquito Bulletin, 13, 1

Linton Y.-M. , Smith L. , Koliopoulos G. , Zounos A.K. , Samanidou-Voyadjoglou A. , Patsoula E. & Harbach R.E. (2004) The Anopheles maculipennis complex (Diptera: Culicidae) in Greece. Medical and Veterinary Entomology 18 (in press).

Missiroli, 1939, The varieties of Anopheles maculipennis and the malaria problem in Italy, Proceedings of the 7th International Congress of Entomology, 3, 1619

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Bulletin of Entomological Research

Tập 94 Số 6

525-535

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