Molecular genetic diversity within and among German ecotypes in comparison to European perennial ryegrass cultivars

Plant Breeding - Tập 124 Số 3 - Trang 257-262 - 2005
Snježana Bolarić1, Susanne Barth2, Albrecht E. Melchinger3, K. Ulrich Posselt1
1University of Hohenheim, State Plant Breeding Institute, D‐70593 Stuttgart, Germany
2Teagasc, Crops Research Centre, Oak Park, Carlow, Ireland
3University of Hohenheim, Institute of Plant Breeding, Population Genetics and Seed Science, D‐70593 Stuttgart, Germany

Tóm tắt

AbstractPerennial ryegrass (Lolium perenne L.) is the most important grass species for temperate grassland agriculture. The level and distribution of genetic variation in gene bank ecotype collections is still largely unknown but of great interest for the planning of breeding programmes. The objectives of this study were to (i) assess the molecular variance and population structure of German ecotypes at the regional and population level, (ii) assign ecotypes to germplasm pools and (iii) compare the relationship between German ecotypes and previously‐investigated European cultivars of perennial ryegrass, A total of 22 ecotypes originating from three geographic areas in Germany. each with a sample size of 20 individual plants, were investigated with 156 polymorphic RAPD markers. Genetic distance among ecotypes ranged from 0,27 to 0.48, An analysis of molecular variance (AMOVA) revealed a much larger variation within populations (71%) than among them (29%). Ecotypes from North Germany were significantly different from those of South and Middle Germany. Thus, two distinct germplasm pools could be identified. The 22 ecotypes and 22 previously investigated cultivars shared 98% of the molecular variance.

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Plant Breeding

Tập 124 Số 3

257-262

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