Micropropagation of Vitis vinifera L. cv. ‘Malagouzia’ and ‘Xinomavro’

Central European Journal of Biology - Tập 5 - Trang 839-852 - 2010
Fotini G. Skiada1, Katerina Grigoriadou2, Eleftherios P. Eleftheriou1
1Department of Botany, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece
2Vitro Hellas S.A., Niseli Imathias, Alexandria, Greece

Tóm tắt

The effects of six basal media on in vitro shoot proliferation of the greek grapevines Vitis vinifera L. cv. ‘Malagouzia’ and ‘Xinomavro’ were investigated. Galzy and Zlenco proved to be the most effective for ‘Malagouzia’ and ‘Xinomavro’, respectively. If only BA was present in the medium, shoot development was poor and the plantlets were chlorotic. When the medium was supplemented with BA and NAA, growth was enhanced. The best ratio (in μM) of growth regulators was 0.5/0.3 for ‘Malagouzia’, and 0.1/0.03 for ‘Xinomavro’, which resulted in the highest number of microshoots per explant and greatest proliferation rate. The development of ‘Malagouzia’ and ‘Xinomavro’ explants at 21±2 and 26±2°C was also investigated, revealing the higher temperature to be more effective. Regarding rooting, 0.5 μM IBA improved root formation at 26°C for ‘Malagouzia’ and 0.5 μM IBA at 21°C for ‘Xinomavro’. Moreover, 0.5 μM IBA resulted in a higher rooting percentage (>95%) and proved to be more beneficial for the overall morphological appearance of the plantlets of ‘Malagouzia’. After acclimatization, survival of microshoots cultivated in media with IBA was higher than those in NAA.

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Central European Journal of Biology

Tập 5

839-852

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