Metabolite extraction from adherently growing mammalian cells for metabolomics studies: optimization of harvesting and extraction protocols
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Dettmer K, Aronov PA, Hammock BD (2007) Mass spectrometry-based metabolomics. Mass Spectrom Rev 26(1):51–78
Theobald U, Mailinger W, Reuss M, Rizzi M (1993) In vivo analysis of glucose-induced fast changes in yeast adenine nucleotide pool applying a rapid sampling technique. Anal Biochem 214(1):31–37
Villas-Boas SG, Hojer-Pedersen J, Akesson M, Smedsgaard J, Nielsen J (2005) Global metabolite analysis of yeast: evaluation of sample preparation methods. Yeast 22(14):1155–1169
Ritter JB, Genzel Y, Reichl U (2008) Simultaneous extraction of several metabolites of energy metabolism and related substances in mammalian cells: optimization using experimental design. Anal Biochem 373(2):349–369
Bennett BD, Yuan J, Kimball EH, Rabinowitz JD (2008) Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach. Nat Protoc 3(8):1299–1311
Hofmann U, Maier K, Niebel A, Vacun G, Reuss M, Mauch K (2008) Identification of metabolic fluxes in hepatic cells from transient 13c-labeling experiments: part I. Exp Observations Biotechnol Bioeng 100(2):344–354
Teng Q, Huang WL, Collette TW, Ekman DR, Tan C (2009) A direct cell quenching method for cell-culture based metabolomics. Metabolomics 5(2):199–208
Maharjan RP, Ferenci T (2003) Global metabolite analysis: the influence of extraction methodology on metabolome profiles of escherichia coli. Anal Biochem 313(1):145–154
Timischl B, Dettmer K, Kaspar H, Thieme M, Oefner PJ (2008) Development of a quantitative, validated capillary electrophoresis-time of flight-mass spectrometry method with integrated high-confidence analyte identification for metabolomics. Electrophoresis 29(10):2203–2214
Hiller J, Franco-Lara E, Weuster-Botz D (2007) Metabolic profiling of escherichia coli cultivations: evaluation of extraction and metabolite analysis procedures. Biotechnol Lett 29(8):1169–1178
Winder CL, Dunn WB, Schuler S, Broadhurst D, Jarvis R, Stephens GM, Goodacre R (2008) Global metabolic profiling of escherichia coli cultures: an evaluation of methods for quenching and extraction of intracellular metabolites. Anal Chem 80(8):2939–2948
Rabinowitz JD, Kimball E (2007) Acidic acetonitrile for cellular metabolome extraction from escherichia coli. Anal Chem 79(16):6167–6173
Sellick C, Knight D, Croxford A, Maqsood A, Stephens G, Goodacre R, Dickson A (2010) Evaluation of extraction processes for intracellular metabolite profiling of mammalian cells: matching extraction approaches to cell type and metabolite targets. Metabolomics 6(3):427–438
Kaspar H, Dettmer K, Gronwald W, Oefner PJ (2008) Automated GC-MS analysis of free amino acids in biological fluids. J Chromatogr B Analyt Technol Biomed Life Sci 870(2):222–232
F.A.D.A. U. S. Department of Health and Human Services CfDEaR, Center for Veterinary Medicine (2001) Guidance for industry: bioanalytical method validation.
Klein MS, Almstetter MF, Schlamberger G, Nurnberger N, Dettmer K, Oefner PJ, Meyer HH, Wiedemann S, Gronwald W (2010) Nuclear magnetic resonance and mass spectrometry-based milk metabolomics in dairy cows during early and late lactation. J Dairy Sci 93(4):1539–1550
R-Development CoreTeam (2007) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria
Halket JM, Waterman D, Przyborowska AM, Patel RK, Fraser PD, Bramley PM (2005) Chemical derivatization and mass spectral libraries in metabolic profiling by GC/MS and LC/MS/MS. J Exp Bot 56(410):219–243
Steinhauser D, Kopka J (2007) Methods, applications and concepts of metabolite profiling: primary metabolism. EXS 97:171–194