Metabolism of toluene and xylenes by Pseudomonas (putida (arvilla) mt-2: evidence for a new function of the TOL plasmid

Journal of Bacteriology - Tập 124 Số 1 - Trang 7-13 - 1975
M J Worsey, Peter A. Williams

Tóm tắt

Pseudomonas putida (arvilla) mt-2 carries genes for the catabolism of toluene, m-xylene, and p-xylene on a transmissible plasmid, TOL. These compounds are degraded by oxidation of one of the methyl substituents via the corresponding alcohols and aldehydes to benzoate and m- and p-toluates, respectively, which are then further metabolised by the meta pathway, also coded for by the TOL plasmid. The specificities of the benzyl alcohol dehydrogenase and the benzaldehyde dehydrogenase for their three respective substrates are independent of the carbon source used for growth, suggesting that a single set of nonspecific enzymes is responsible for the dissimilation of the breakdown products of toluene and m- and p-xylene. Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase are coincidently and possible coordinately induced by toluene and the xylenes, and by the corresponding alcohols and aldehydes. They are not induced in cells grown on m-toluate but catechol 2,3-oxygenase can be induced by m-xylene.

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Tài liệu tham khảo

Chakrabarty , A. M. 1972 . Genetic basis of the biodegradation of salicylate in Pseudomonas . J. Bacteriol. 112 : 815 - 823 .

Davey , J. F. , and D. T. Gibson , 1974 . Bacterial metabolism of para- and meta-xylene: oxidation of a methyl substituent . J. Bacteriol. 119 : 923 - 929 .

Davis , R. S. , F. E. Hossler , and R. W. Stone , 1968 . Metabolism of p- and m-xylene by a species of Pseudomonas . Can. J. Microbiol. 14 : 1005 - 1009 .

Dunn , N. W. , and I. C. Gunsalus . 1973 . Transmissible plasmid coding early enzymes of naphthalene oxidation in Pseudomonas putida . J. Bacteriol. 114 : 974 - 979 .

Murray , K. , C. J. Duggleby , J. M. Sala-Trepat , and P. A. Williams . 1972 . The metabolism of benzoate and methyl benzoates via the meta-cleavage pathway by Pseudomonas arvilia mt-2 . Eur. J. Biochem. 28 : 301 - 310 .

Murray , K. , and P. A. Williams . 1974 . Role of catechol and methyl catechols as inducers of aromatic metabolism in Pseudomonas putida . J. Bacteriol. 117 : 1153 - 1157 .

Omori , T. , S. Horiguchi , and K. Yamada . 1967 . Studies on the utilisation of hydrocarbons by micro-organisms. Part X. Screening of aromatic hydrocarbon-assimilating micro-organisms and p-toluic acid formation from p-xylene . Agric. Biol. Chem. 31 : 1337 - 1342 .

Omori , T. , and K. Yamada . 1969 . Studies on the utilisation of hydrocarbons by micro-organisms. Part XIII. Oxidation of m-xylene and pseudocumene by Pseudomonas aeruginosa . Agric. Biol. Chem. 33 : 659 - 663 .

Ornston , L. N. 1966 . The conversion of catechol and protocatechuate to ,-ketoadipate by Pseudomonas putida IV . Regulation. J. Biol. Chem. 241 : 3800 - 3810 .

Ornston , L. N. 1971 . Regulation of catabolic pathways in Pseudomonas . Bacteriol. Rev. 35 : 87 - 116 .

Ornston , L. N. , M. K. Ornston , and G. Chou . 1969 . Isolation of spontaneous mutant strains of Pseudomonas putida . Biochem. Biophys. Res. Commun. 36 : 179 - 184 .

Sala-Trepat , J. M. , and W. C. Evans . 1971 . The meta cleavage of catechol by Azotobacter species . Eur. J. Biochem. 20 : 400 - 413 .

Sala-Trepat , J. M. , K. Murray , and P. A. Williams . 1972 . The metabolic divergence in the meta cleavage of catechols by Pseudomonos putida NCIB 10015. Physiological significance and evolutionary implications . Eur. J. Biochem. 28 : 347 - 356 .

Stanier , R. Y. , N. J. Palleroni , and M. Doudoroff . 1966 . The aerobic pseudomonads: a taxonomic study . J. Gen. Microbiol. 43 : 159 - 273 .

Wigmore , G. J. , R. C. Bayly , and D. Di Berardino . 1974 . Pseudomomas putida mutants defective in the metabolism of the products of meta fission of catechol and its methyl analogues . J. Bacteriol. 120 : 31 - 37 .

Williams , P. A. , and K. Murray 1974 . Metabolism of benzoate and the methyl benzoates by Pseudomonas putida (arvilla) mt-2: Evidence for the existence of a TOL plasmid . J. Bacteriol. 120 : 416 - 423 .

Wong , C. L. , and N. W. Dunn . 1974 . Transmissible plasmid coding for the degradation of benzoate and m-toluate in Pseudomonas arvilla mt-2 . Genet. Res. 23 : 227 - 232 .

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Journal of Bacteriology

Tập 124 Số 1

7-13

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