MYB class transcription factors bind to the tuber-specific and sucrose-response element of a class-I patatin promoter

Plant Biotechnology Reports - Tập 11 - Trang 239-245 - 2017
Hyung-in Choi1, Sun-Young Baek1, Soo Young Kim1
1Department of Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, South Korea

Tóm tắt

Class-I patatin genes, which encode the potato storage protein patatin, are tuber specific in their expression, and several promoter elements directing the tissue-specific expression of the patatin genes have been known for long time. However, the identity of the relevant transcription factors is still unknown. In this study, we show that K box, one of the protein-binding sites in a class-I patatin gene promoter, is a novel cis-regulatory element required for the tuber-specific expression of the gene. Subsequently, we isolated K-box-binding factors by yeast one-hybrid screening. Analysis of the factors revealed that they are MYB class transcription factors, which we named TSF1–4 (tuber-specific and sucrose-inducible element-binding factors 1–4). TSFs possess transcriptional activity to various degrees, and overexpression of TSF2, which possesses the highest transcriptional activity, induced the ectopic expression of patatin genes in potato leaves. Our results strongly suggest that TSFs could play regulatory function in the expression of patatin genes in planta.

Tài liệu tham khảo

Andrews DL, Beames B, Summers MD, Park WD (1988) Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector. Biochem J 252:199–206 Bevan M, Barker R, Goldsbrough A, Jarvis M, Kavanagh T, Iturriaga G (1986) The structure and transcription start site of a major potato tuber protein gene. Nucleic Acids Res 14:4625–4638 Choi H, Hong J, Ha J, Kang J, Kim SY (2000) ABFs, a family of ABA-responsive element binding factors. J Biol Chem 275:1723–1730 Chomczynski P, Mackey K (1995) Modification of the TRI reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources. Biotechniques 19:942–945 Fu H, Kim SY, Park WD (1995) High-level tuber expression and sucrose inducibility of a potato Sus4 sucrose synthase gene require 5′ and 3′ flanking sequences and the leader intron. Plant Cell 7:1387–1394 Grierson C, Du JS, de Torres Zabala M, Beggs K, Smith C, Holdsworth M, Bevan M (1994) Separate cis sequences and trans factors direct metabolic and developmental regulation of a potato tuber storage protein gene. Plant J 5:815–826 Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 20:3901–3907 Jefferson R, Goldsbrough A, Bevan M (1990) Transcriptional regulation of a patatin-1 gene in potato. Plant Mol Biol 14:995–1006 Jin HL, Martin C (1999) Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol 41:577–585. doi:10.1023/A:1006319732410 Kang J, Choi H, Im M, Kim SY (2002) Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell 14:343–357 Kim SY, Thomas TL (1998) A family of novel basic leucine zipper proteins bind to seed-specification elements in the carrot Dc3 gene promoter. J Plant Physiol 152:607–613 Kim SY, May GD, Park WD (1994) Nuclear protein factors binding to a class I patatin promoter region are tuber-specific and sucrose-inducible. Plant Mol Biol 26:603–615 Kim SY, Chung H-J, Thomas TL (1997) Isolation of a novel class of bZIP transcription factors that bind to the ABA-responsive and embryo-specification elements in the Dc3 promoter region using a modified yeast one-hybrid system. Plant J 11:1237–1251 Koster-Topfer M, Frommer WB, Rocha-Sosa M, Rosahl S, Schell J, Willmitzer L (1989) A class II patatin promoter is under developmental control in both transgenic potato and tobacco plants. Mol Gen Genet 219:390–396 Liu XJ, Prat S, Willmitzer L, Frommer WB (1990) cis regulatory elements directing tuber-specific and sucrose-inducible expression of a chimeric class I patatin promoter/GUS-gene fusion. Mol Gen Genet 223:401–406 Liu XY, Rocha-Sosa M, Hummel S, Willmitzer L, Frommer WB (1991) A detailed study of the regulation and evolution of the two classes of patatin genes in Solanum tuberosum L. Plant Mol Biol 17:1139–1154 Mignery GA, Pikaard CS, Park WD (1988) Molecular characterization of the patatin multigene family of potato. Gene 62:27–44 Paiva E, Lister RM, Park WD (1983) Induction and accumulation of major tuber proteins of potato in stems and petioles. Plant Physiol 71:161–168 Pikaard CS, Brusca JS, Hannapel DJ, Park WD (1987) The two classes of genes for the major potato tuber protein, patatin, are differentially expressed in tubers and roots. Nucleic Acids Res 15:1979–1994 Rocha-Sosa M, Sonnewald U, Frommer W, Stratmann M, Schell J, Willmitzer L (1989) Both developmental and metabolic signals activate the promoter of a class I patatin gene. EMBO J 8:23–29 Romero I, Fuertes A, Benito MJ, Malpica JM, Leyva A, Paz-Ares J (1998) More than 80R2R3-MYB regulatory genes in the genome of Arabidopsis thaliana. Plant J 14:273–284 Rosahl S, Schimidt R, Schell J, Willmitzer L (1986) Isolation and characterization of a gene from Solanum tuberosum encoding patatin, the major storage protein of potato tubers. Mol Gen Genet 203:214–220 Rosahl S, Schell J, Willmitzer L (1987) Expression of a tuber-specific storage protein in transgenic tobacco plants: demonstration of an esterase activity. EMBO J 6:1155–1159 Stracke R, Werber M, Weisshaar B (2001) The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol 4:447–456 Twell D, Ooms G (1988) Structural diversity of the patatin gene family in potato cv. Desiree. Mol Gen Genet 212:325–336 Wenzler H, Mignery G, Fisher L, Park W (1989) Sucrose-regulated expression of a chimeric potato tuber gene in leaves of transgenic tobacco plants. Plant Mol Biol 13:347–354 Yanhui C, Xiaoyuan Y, Kun H, Meihua L, Jigang L, Zhaofeng G, Zhiqiang L, Yunfei Z, Xiaoxiao W, Xiaoming Q, Yunping S, Li Z, Xiaohui D, Jingchu L, Xing-Wang D, Zhangliang C, Hongya G, Li-Jia Q (2006) The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family. Plant Mol Biol 60:107–124. doi:10.1007/s11103-005-2910-y Zourelidou M, de Torres-Zabala M, Smith C, Bevan MW (2002) Storekeeper defines a new class of plant-specific DNA-binding proteins and is a putative regulator of patatin expression. Plant J 30:489–497