Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium

Gastroenterology - Tập 141 Số 5 - Trang 1762-1772 - 2011
Toshiro Sato1, Daniel E. Stange1, Marc Ferrante2,3,1, Robert G.J. Vries1, Johan H. van Es1, Stieneke van den Brink1, Winan J. van Houdt4,5, Apollo Pronk4, Joost van Gorp6, Peter D. Siersema3, Hans Clevers1
1Hubrecht Institute–KNAW and University Medical Centre Utrecht, Utrecht, The Netherlands
2Department of Gastroenterology, University Hospital Gasthuisberg, Leuven, Belgium
3Gastrointestinal Research Unit, Department of Gastroenterology and Hepatology, University Medical Centre Utrecht, Utrecht, The Netherlands
4Department of Surgery, Diakonessenhuis, Utrecht, The Netherlands
5Department of Surgical Oncology, University Medical Centre Utrecht, Utrecht, The Netherlands
6Department of Pathology, Diakonessenhuis, Utrecht, The Netherlands

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Evans, 1992, The development of a method for the preparation of rat intestinal epithelial cell primary cultures, J Cell Sci, 101, 219, 10.1242/jcs.101.1.219

Fukamachi, 1992, Proliferation and differentiation of fetal rat intestinal epithelial cells in primary serum-free culture, J Cell Sci, 103, 511, 10.1242/jcs.103.2.511

Perreault, 1996, Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures, Exp Cell Res, 224, 354, 10.1006/excr.1996.0145

Whitehead, 1999, Clonogenic growth of epithelial cells from normal colonic mucosa from both mice and humans, Gastroenterology, 117, 858, 10.1016/S0016-5085(99)70344-6

Ootani, 2009, Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche, Nat Med, 15, 701, 10.1038/nm.1951

Sato, 2009, Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche, Nature, 459, 262, 10.1038/nature07935

Korinek, 1998, Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4, Nat Genet, 19, 379, 10.1038/1270

Pinto, 2003, Canonical Wnt signals are essential for homeostasis of the intestinal epithelium, Genes Dev, 17, 1709, 10.1101/gad.267103

Kuhnert, 2004, Essential requirement for Wnt signaling in proliferation of adult small intestine and colon revealed by adenoviral expression of Dickkopf-1, Proc Natl Acad Sci U S A, 101, 266, 10.1073/pnas.2536800100

Kim, 2005, Mitogenic influence of human R-spondin1 on the intestinal epithelium, Science, 309, 1256, 10.1126/science.1112521

Barker, 2007, Identification of stem cells in small intestine and colon by marker gene Lgr5, Nature, 449, 1003, 10.1038/nature06196

de Lau, 2011, Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling, Nature, 476, 293, 10.1038/nature10337

Dignass, 2001, Peptide growth factors in the intestine, Eur J Gastroenterol Hepatol, 13, 763, 10.1097/00042737-200107000-00002

Haramis, 2004, De novo crypt formation and juvenile polyposis on BMP inhibition in mouse intestine, Science, 303, 1684, 10.1126/science.1093587

Hofmann, 2007, Cell-cell contacts prevent anoikis in primary human colonic epithelial cells, Gastroenterology, 132, 587, 10.1053/j.gastro.2006.11.017

Sasaki, 2002, Expression and distribution of laminin alpha1 and alpha2 chains in embryonic and adult mouse tissues: an immunochemical approach, Exp Cell Res, 275, 185, 10.1006/excr.2002.5499

Rogler, 2001, Differential activation of cytokine secretion in primary human colonic fibroblast/myofibroblast cultures, Scand J Gastroenterol, 36, 389, 10.1080/003655201300051216

Buset, 1987, Defining conditions to promote the attachment of adult human colonic epithelial cells, In Vitro Cell Dev Biol, 23, 403, 10.1007/BF02623855

Whitehead, 1987, A method for the isolation and culture of human colonic crypts in collagen gels, In Vitro Cell Dev Biol, 23, 436, 10.1007/BF02623860

Deveney, 1996, Establishment of human colonic epithelial cells in long-term culture, J Surg Res, 64, 161, 10.1006/jsre.1996.0323

Pang, 1996, Immunologic, functional, and morphological characterization of three new human small intestinal epithelial cell lines, Gastroenterology, 111, 8, 10.1053/gast.1996.v111.pm8698229

Latella, 1996, Characterization of the mucins produced by normal human colonocytes in primary culture, Int J Colorectal Dis, 11, 76, 10.1007/BF00342464

Panja, 2000, A novel method for the establishment of a pure population of nontransformed human intestinal primary epithelial cell (HIPEC) lines in long term culture, Lab Invest, 80, 1473, 10.1038/labinvest.3780154

Grossmann, 2003, Progress on isolation and short-term ex-vivo culture of highly purified non-apoptotic human intestinal epithelial cells (IEC), Eur J Cell Biol, 82, 262, 10.1078/0171-9335-00312

Sansom, 2004, Loss of Apc in vivo immediately perturbs Wnt signaling, differentiation, and migration, Genes Dev, 18, 1385, 10.1101/gad.287404

Lustig, 2002, Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors, Mol Cell Biol, 22, 1184, 10.1128/MCB.22.4.1184-1193.2002

Booth, 2002, Isolation and culture of intestinal epithelial cells, 303

Gregorieff, 2005, Expression pattern of Wnt signaling components in the adult intestine, Gastroenterology, 129, 626, 10.1016/j.gastro.2005.06.007

Sato, 2011, Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts, Nature, 469, 415, 10.1038/nature09637

van de Wetering, 2002, The beta-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells, Cell, 111, 241, 10.1016/S0092-8674(02)01014-0

Denu, 2005, Vitamin B3 and sirtuin function, Trends Biochem Sci, 30, 479, 10.1016/j.tibs.2005.07.004

Shay, 2006, Telomerase therapeutics for cancer: challenges and new directions, Nat Rev Drug Discov, 5, 577, 10.1038/nrd2081

Otsuka, 2010, Distinct effects of p38alpha deletion in myeloid lineage and gut epithelia in mouse models of inflammatory bowel disease, Gastroenterology, 138, 1255, 10.1053/j.gastro.2010.01.005

van Es, 2005, Notch/gamma-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells, Nature, 435, 959, 10.1038/nature03659

Barker, 2009, Crypt stem cells as the cells-of-origin of intestinal cancer, Nature, 457, 608, 10.1038/nature07602

Odze, 2009, Barrett esophagus: histology and pathology for the clinician, Nat Rev Gastroenterol Hepatol, 6, 478, 10.1038/nrgastro.2009.103

Menke, 2010, Conversion of metaplastic Barrett's epithelium into post-mitotic goblet cells by gamma-secretase inhibition, Dis Model Mech, 3, 104, 10.1242/dmm.003012

Dey, 2009, Phenotypic and functional characterization of human mammary stem/progenitor cells in long term culture, PloS One, 4, e5329, 10.1371/journal.pone.0005329

Garraway, 2010, Human prostate sphere-forming cells represent a subset of basal epithelial cells capable of glandular regeneration in vivo, Prostate, 70, 491, 10.1002/pros.21083

Walen, 2004, Spontaneous cell transformation: karyoplasts derived from multinucleated cells produce new cell growth in senescent human epithelial cell cultures, In Vitro Cell Dev Biol Anim, 40, 150, 10.1290/1543-706X(2004)40<150:SCTKDF>2.0.CO;2

Hayflick, 1979, The cell biology of aging, J Invest Dermatol, 73, 8, 10.1111/1523-1747.ep12532752