Ligand-Binding Mass Spectrometry to Study Biotransformation of Fusion Protein Drugs and Guide Immunoassay Development: Strategic Approach and Application to Peptibodies Targeting the Thrombopoietin Receptor

Cummings SR, San Martin J, McClung MR, Siris ES, Eastell R, Reid IR, et al. Denosumab for prevention of fractures in postmenopausal women with osteoporosis. N Engl J Med. 2009;361(8):756–65.

Banerjee S, Flores-Rozas H. Monoclonal antibodies for targeted therapy in colorectal cancer. Cancer Biol Ther. 2010;9(8):563–71.

Klettner A, Roider J. Treating age-related macular degeneration—interaction of VEGF-antagonists with their target. Mini Rev Med Chem. 2009;9(9):1127–35.

Suzuki T, Ishii-Watabe A, Tada M, Kobayashi T, Kanayasu-Toyoda T, Kawanishi T, et al. Importance of neonatal FcR in regulating the serum half-life of therapeutic proteins containing the Fc domain of human IgG1: a comparative study of the affinity of monoclonal antibodies and Fc-fusion proteins to human neonatal FcR. J Immunol. 2010;184(4):1968–76.

Peters RT, Low SC, Kamphaus GD, Dumont JA, Amari JV, Lu Q, et al. Prolonged activity of factor IX as a monomeric Fc fusion protein. Blood. 2010;115(10):2057–64.

Escher SE, Forssmann U, Frimpong-Boateng A, Adermann K, Vakili J, Sticht H, et al. Functional analysis of chemically synthesized derivatives of the human CC chemokine CCL15/HCC-2, a high affinity CCR1 ligand. J Pept Res. 2004;63(1):36–47.

Pernemalm M, Orre LM, Lengqvist J, Wikstrom P, Lewensohn R, Lehtio J. Evaluation of three principally different intact protein prefractionation methods for plasma biomarker discovery. J Proteome Res. 2008;7(7):2712–22.

Anderson NL, Jackson A, Smith D, Hardie D, Borchers C, Pearson TW. SISCAPA peptide enrichment on magnetic beads using an in-line bead trap device. Mol Cell Proteomics. 2009;8(5):995–1005.

Bandow JE. Comparison of protein enrichment strategies for proteome analysis of plasma. Proteomics. 2010;10(7):1416–25.

Wang H, Hanash S. Electrospray mass spectrometry for quantitative plasma proteome analysis. Meth Mol Biol. 2009;564:227–42.

Wang H, Hanash S. Intact-protein based sample preparation strategies for proteome analysis in combination with mass spectrometry. Mass Spectrom Rev. 2005;24(3):413–26.

Lu Q, Zheng X, McIntosh T, Davis H, Nemeth JF, Pendley C, et al. Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Anal Chem. 2009;81(21):8715–23.

Molineux G, Newland A. Development of romiplostim for the treatment of patients with chronic immune thrombocytopenia: from bench to bedside. Br J Haematol. 2010;17:1365–2141.

Cersosimo RJ. Romiplostim in chronic immune thrombocytopenic purpura. Clin Ther. 2009;31(9):1887–907.

Nedelkov D. Mass spectrometry-based immunoassays for the next phase of clinical applications. Expert Rev Proteomics. 2006;3(6):631–40.