Lactate Dehydrogenase Isozymes: Dissociation and Recombination of Subunits

American Association for the Advancement of Science (AAAS) - Tập 140 Số 3573 - Trang 1329-1330 - 1963
Clement L. Markert1
1Department of Biology, Johns Hopkins University, Baltimore 18, Maryland

Tóm tắt

Lactate dehydrogenase from beef tissues may be resolved electrophoretically into five isozymes each of which is a tetramer. These tetramers can be dissociated into monomers by freezing in 1 M sodium chloride. On thawing, reassociation into functional tetramers occurs. On the basis of charge and amino acid composition there are two kinds of monomers. Lactate dehydrogenase-1 contains one kind of monomer and lactate dehydrogenase-5 the other kind. A mixture of equal quantities of these two isozymes, after dissociation and reassociation, leads to the production of all five isozymes in the expected proportions of 1:4:6:4:1.

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Tài liệu tham khảo

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American Association for the Advancement of Science (AAAS)

Tập 140 Số 3573

1329-1330

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