Isolation and in vitro propagation of human choroidal fibroblasts

Wiley - Tập 74 Số 1 - Trang 26-30 - 1996
Per Klyve1, Bjørn Nicolaissen2, O Naess2
1Department of Ophthalmology, Ullevål University Hospital, University of Oslo, Norway.
2Departments of Ophthalmology and Pathology, Ullevål University Hospital, University of Oslo, Oslo, Norway

Tóm tắt

ABSTRACT Subretinal ingrowth of choroidal fibroblasts is of importance in several clinical settings such as age‐related macular degeneration or after traumas such as laser treatment and infection. In the present investigation we describe a method for isolation and propagation of human choroidal fibroblasts in vitro. Outgrowth from the outseeded choroid was studied by means of phase contrast microscopy, scanning electron microscopy and by immunohistochemical methods. The secondary cultures were found to contain almost only fibroblasts (more than 95% of the cells), some contaminating retinal pigment cells were also found. Choroidal fibroblasts contribute to the fibrovascular subretinal scarring process, and our cell culturing system seems to be a suitable tool for studying factors regulating the behaviour of these cells in vitro.

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Tài liệu tham khảo

10.1001/archopht.1992.01080180099035

10.1007/978-1-4899-5292-9_32

10.1097/00006982-199111020-00010

10.1111/j.1755-3768.1992.tb08567.x

10.1001/archopht.1992.01080180105036

Hu D‐N, 1993, Studies of human uveal melanocytes in vitro: Isolation, purification and cultivation of human uveal melanocytes, Invest Ophthalmol Vis Sci, 34, 2210

Korte G E, 1989, Choriocapillaris regeneration in the rabbit, Invest Ophthalmol Vis Sci, 30, 1938

Korte G E, 1984, RPE destruction causes choriocapillary atrophy, Invest Ophthalmol Vis Sci, 25, 1135

Mason D Y, 1985, Immuno‐histochemistry, 25

10.3109/02713689008999578

10.1001/archopht.1989.01070020737038

10.1111/j.1755-3768.1988.tb04050.x

10.1159/000310075

10.1111/j.1755-3768.1988.tb04051.x

10.1001/archopht.1982.01030040784015

Tso‐M O M, 1973, Organ culture of human retinal pigment epithelium and choroid: a model for the study of cytologic behavior of RPE in vitro, Invest Ophthalmol Vis Sci, 12, 554

10.1242/jcs.50.1.45

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Thông tin xuất bản

Wiley

Tập 74 Số 1

26-30

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