Isolation and characterization of two distinct lectins with antiproliferative activity from the cultured mycelium of the edible mushroom Tricholoma mongolicum

Wiley - Tập 46 Số 6 - Trang 508-513 - 1995
H. X. Wang1, T.B. Ng2, Wei–Ting Liu3, V.E.C. OOU1, Song Chang1
1Departments of Biology, The Chinese University of Hong Kong, New Territories, Hong Kong
2Departments of Biochemistry, The Chinese University of Hong Kong, New Territories, Hong Kong
3Departments of Anatomy, The Chinese University of Hong Kong, New Territories, Hong Kong

Tóm tắt

Two lectins, TML‐1 and TML‐2, were isolated from Tricholoma mongolicum by ion‐exchange chromatography and gel filtration. They are dimers with molecular weight near 37 000. The hemagglutinating activities of TML‐1 and TML‐2 are sensitive to lactose inhibition and are stable between 10 and 80 °C. They exhibit antiproliferative activities against mouse monocyte‐macrophage PU5–1.8 cells and mouse mastocytoma P815 cells in vitro. The two lectins differ in the content of proline and tyrosine residues. Both are non‐glycoproteins and have hydroxyproline residues. © Munksgaard 1995.

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Tài liệu tham khảo

10.1159/000226417

Konska G., 1994, J. Biochem., 116, 519, 10.1093/oxfordjournals.jbchem.a124555

10.1126/science.177.4053.949

10.1093/oxfordjournals.jbchem.a134826

10.1007/BF00180280

10.1016/0304-4165(90)90045-X

10.1038/227292a0

Hajto T., 1990, Cancer Res., 50, 3322

Beuth J., 1991, In Vivo, 5, 29

10.1159/000239204

Yu L.G., 1993, Cancer Res., 53, 4621

10.1016/0008-6215(88)80057-0

10.1016/S0953-7562(09)80455-3

10.1016/0014-5793(94)80172-X

Yeung H.W., 1986, Int. J. Peptide Protein Res., 21, 208

10.1104/pp.101.2.513

10.1016/0020-711X(93)90632-O

10.1006/bbrc.1994.1241

10.1016/0003-2697(71)90438-6

10.1016/0162-3109(93)90006-C

10.1016/0014-5793(88)80876-7

10.1016/0304-4165(78)90358-6

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Wiley

Tập 46 Số 6

508-513

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