Isolation and characterization of polymorphic microsatellites in olive (Olea europaea L.) and their transferability to other genera in the Oleaceae

Wiley - Tập 2 Số 3 - Trang 265-267 - 2002
Raúl de la Rosa1, C. M. James1, K. R. Tobutt1
1Horticultural Research International‐East Malling, East Malling, West Malling, Kent ME19 6BJ, UK

Tóm tắt

AbstractSeven polymorphic microsatellites were developed in olive. Six of them came from a genomic library enriched for GA and CA repeat sequences. They showed single locus polymorphism in a set of 23 olive cultivars (from six to nine alleles per locus). Three different pairs of loci were sufficient to discriminate all cultivars. The other polymorphic primer pair was designed from a published sequence for olive lupeol sgutase and revealed just two alleles. The seven primer pairs were tested on two accessions of five other species of the Oleaceae and three, EMO2, EMO13 and EMO90, revealed polymorphism in two, four and three species, respectively.

Từ khóa


Tài liệu tham khảo

Belaj A, 2001, Polymorphism and discrimination capacity of randomly amplified polymorphic markers in an olive germplasm bank, Journal of American Society of Horticultural Science, 126, 64, 10.21273/JASHS.126.1.64

Edwards KJ, 1996, Microsatellite libraries enriched for several microsatellite sequences in plants, Biotechniques, 30, 758, 10.2144/96205bm04

10.1093/nar/8.19.4321

10.1046/j.1365-294x.2000.00954.x

10.1046/j.1432-1327.1999.00875.x

Thông tin
Thông tin xuất bản

Wiley

Tập 2 Số 3

265-267

Thông tin tác giả