Isolation and characterization of a T7-like lytic phage for Pseudomonas fluorescens

Springer Science and Business Media LLC - Tập 8 - Trang 1-11 - 2008
Sanna Sillankorva1,2, Peter Neubauer2, Joana Azeredo3
1IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Braga, Portugal
2Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, Oulu, Finland
3IBB – Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Braga, Portugal

Tóm tắt

Despite the proven relevance of Pseudomonas fluorescens as a spoilage microorganism in milk, fresh meats and refrigerated food products and the recognized potential of bacteriophages as sanitation agents, so far no phages specific for P. fluorescens isolates from dairy industry have been closely characterized in view of their lytic efficiency. Here we describe the isolation and characterization of a lytic phage capable to infect a variety of P. fluorescens strains isolated from Portuguese and United States dairy industries. Several phages were isolated which showed a different host spectrum and efficiency of lysis. One of the phages, phage ϕIBB-PF7A, was studied in detail due to its efficient lysis of a wide spectrum of P. fluorescens strains and ribotypes. Phage ϕIBB-PF7A with a head diameter of about 63 nm and a tail size of about 13 × 8 nm belongs morphologically to the Podoviridae family and resembles a typical T7-like phage, as analyzed by transmission electron microscopy (TEM). The phage growth cycle with a detected latent period of 15 min, an eclipse period of 10 min, a burst size of 153 plaque forming units per infected cell, its genome size of approximately 42 kbp, and the size and N-terminal sequence of one of the protein bands, which gave similarity to the major capsid protein 10A, are consistent with this classification. The isolated T7-like phage, phage ϕIBB-PF7A, is fast and efficient in lysing different P. fluorescens strains and may be a good candidate to be used as a sanitation agent to control the prevalence of spoilage causing P. fluorescens strains in dairy and food related environments.

Tài liệu tham khảo

Arnaut-Rollier I, De Zutter L, Van Hoof J: Identities of the Pseudomonas spp. in flora from chilled chicken. International Journal of Food Microbiology. 1999, 48: 87-96. 10.1016/S0168-1605(99)00038-0.

Bodilis J, Calbrix R, Guerillon J, Merieau A, Pawlak B, Orange N, Barray S: Phylogenetic relationships between environmental and clinical isolates of Pseudomonas fluorescens and related species deduced from 16S rRNA gene and OprF protein sequences. Systematic and Applied Microbiology. 2004, 27: 93-108. 10.1078/0723-2020-00253.

Dogan B, Boor KJ: Genetic diversity and spoilage potentials among Pseudomonas spp. isolated from fluid milk products and dairy processing plants. Applied and Environmental Microbiology. 2003, 69: 130-138. 10.1128/AEM.69.1.130-138.2003.

Peneau S, Chassaing D, Carpentier B: First evidence of division and accumulation of viable but nonculturable Pseudomonas fluorescens cells on surfaces subjected to conditions encountered at meat processing premises. Applied and Environmental Microbiology. 2007, 73: 2839-2846. 10.1128/AEM.02267-06.

Wang L, Jayarao BM: Phenotypic and genotypic characterization of Pseudomonas fluorescens isolated from bulk tank milk. Journal of Dairy Science. 2001, 84: 1421-1429.

Morgan AD, Brockhurst MA, Lopez-Pascua LDC, Pal C, Buckling A: Differential impact of simultaneous migration on coevolving hosts and parasites. Bmc Evolutionary Biology. 2007, 7:

Buckling A, Wei Y, Massey RC, Brockhurst MA, Hochberg ME: Antagonistic coevolution with parasites increases the cost of host deleterious mutations. Proceedings of the Royal Society B-Biological Sciences. 2006, 273: 45-49. 10.1098/rspb.2005.3279.

Brockhurst MA, Buckling A, Rainey PB: Spatial heterogeneity and the stability of host-parasite coexistence. Journal of Evolutionary Biology. 2006, 19: 374-379. 10.1111/j.1420-9101.2005.01026.x.

Craven HM, Macauley BJ: Microorganisms in Pasteurized Milk After Refrigerated Storage .1. Identification of Types. Australian Journal of Dairy Technology. 1992, 47: 38-45.

Wiedmann M, Weilmeier D, Dineen SS, Ralyea R, Boor KJ: Molecular and phenotypic characterization of Pseudomonas spp. isolated from milk. Applied and Environmental Microbiology. 2000, 66: 2085-2095. 10.1128/AEM.66.5.2085-2095.2000.

Bradley DE: Ultrastructure of Bacteriophages and Bacteriocins. Bacteriological Reviews. 1967, 31: 230-

Kovalyova IV, Kropinski AM: The complete genomic sequence of lytic bacteriophage gh-1 infecting Pseudomonas putida – evidence for close relationship to the T7 group. Virology. 2003, 311: 305-315. 10.1016/S0042-6822(03)00124-7.

Pajunen M, Kiljunen S, Skurnik M: Bacteriophage phi YeO3-12, specific for Yersinia enterocolitica serotype O : 3, is related to coliphages T3 and T7. Journal of Bacteriology. 2000, 182: 5114-5120. 10.1128/JB.182.18.5114-5120.2000.

Barry GT, Goebel WF: The Effect of Chemical and Physical Agents on the Phage Receptor of Phase-Ii Shigella sonnei. Journal of Experimental Medicine. 1951, 94: 387-400. 10.1084/jem.94.5.387.

Arnaut-Rollier I, Vauterin L, De Vos P, Massart DL, Devriese LA, De Zutter L, Van Hoof J: A numerical taxonomic study of the Pseudomonas flora isolated from poultry meat. Journal of Applied Microbiology. 1999, 87: 15-28. 10.1046/j.1365-2672.1999.00785.x.

Cousin MA: Presence and Activity of Psychrotrophic Microorganisms in Milk and Dairy-Products – A Review. Journal of Food Protection. 1982, 45: 172-207.

Cromie S: Psychrotrophs and Their Enzyme Residues in Cheese Milk. Australian Journal of Dairy Technology. 1992, 47: 96-100.

Sorhaug T, Stepaniak L: Psychrotrophs and their enzymes in milk and dairy products: Quality aspects. Trends in Food Science & Technology. 1997, 8: 35-41. 10.1016/S0924-2244(97)01006-6.

Huff WE, Huff GR, Rath NC, Donoghue AM: Evaluation of the influence of bacteriophage titer on the treatment of colibacillosis in broiler chickens. Poult Sci. 2006, 85: 1373-1377.

Atterbury RJ, Van Bergen MA, Ortiz F, Lovell MA, Harris JA, De Boer A, Wagenaar JA, Allen VM, Barrow PA: Bacteriophage therapy to reduce salmonella colonization of broiler chickens. Appl Environ Microbiol. 2007, 73: 4543-4549. 10.1128/AEM.00049-07.

Barrow PA: Novel approaches to control of bacterial infections in animals. Acta Vet Hung. 1997, 45: 317-329.

Higgins JP, Higgins SE, Guenther KL, Huff W, Donoghue AM, Donoghue DJ, Hargis BM: Use of a specific bacteriophage treatment to reduce Salmonella in poultry products. Poult Sci. 2005, 84: 1141-1145.

Loc Carrillo C, Atterbury RJ, el-Shibiny A, Connerton PL, Dillon E, Scott A, Connerton IF: Bacteriophage therapy to reduce Campylobacter jejuni colonization of broiler chickens. Appl Environ Microbiol. 2005, 71: 6554-6563. 10.1128/AEM.71.11.6554-6563.2005.

Sillankorva S, Neubauer P, Azeredo J: Pseudomonas fluorescens biofilms subjected to phage ϕIBB-PF7A. BMC Biotechnology. 2008, 8: 79-10.1186/1472-6750-8-58.

Lavigne R, Burkal'tseva MV, Robben J, Sykilinda NN, Kurochkina LP, Grymonprez B, Jonckx B, Krylov VN, Mesyanzhinov VV, Volckaert G: The genome of bacteriophage phi KMV, a T7-like virus infecting Pseudomonas aeruginosa. Virology. 2003, 312: 49-59. 10.1016/S0042-6822(03)00123-5.

Ceyssens PJ, Lavigne R, Mattheus W, Chibeu A, Hertveldt K, Mast J, Robben J, Volckaert G: Genomic analysis of Pseudomonas aeruginosa phages LKD16 and LKA1: Establishment of the phiKMV subgroup within the T7 supergroup. Journal of Bacteriology. 2006, 188: 6924-6931. 10.1128/JB.00831-06.

Park SC, Shimamura I, Fukunaga M, Mori KI, Nakai T: Isolation of bacteriophages specific to a fish pathogen, Pseudomonas plecoglossicida, as a candidate for disease control. Applied and Environmental Microbiology. 2000, 66: 1416-1422. 10.1128/AEM.66.4.1416-1422.2000.

Park SC, Nakai T: Bacteriophage control of Pseudomonas plecoglossicida infection in ayu Plecoglossus altivelis. Diseases of Aquatic Organisms. 2003, 53: 33-39. 10.3354/dao053033.

Krylov VN: Phagotherapy in terms of bacteriophage genetics: hopes, perspectives, safety, limitations. Genetika. 2001, 37: 869-887.

Sambrook J, Russell DW: Molecular Cloning: A Laboratory Manual. 2001, New York: Cold Spring Harbor Laboratory Press, Cold Spring Har

Adams MH: Bacteriophages. 1959, New York: Interscience Publishers

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227: 680-685. 10.1038/227680a0.

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