Interpretation of the mouse electroretinogram

Springer Science and Business Media LLC - Tập 115 - Trang 127-136 - 2007
Lawrence H. Pinto1, Brandon Invergo1, Kazuhiro Shimomura1, Joseph S. Takahashi1,2, John B. Troy3
1Department of Neurobiology and Physiology and Center for Functional Genomics, Northwestern University, Evanston, USA
2Howard Hughes Medical Institute, Northwestern University, Evanston, USA
3Department of Biomedical Engineering, Technological Institute, Northwestern University, Evanston, USA

Tóm tắt

The mouse electroretinogram (ERG) consists of a complex set of signals or “waves” generated by multiple types of retinal cell. The origins of these waves are reviewed briefly for the C57BL/6J mouse. The differences in the properties of these waves are described for 34 strains of mice and 11 F1 hybrid mice, as is the way that inter-strain genetic polymorphisms can be exploited in order to help pin-point the genes responsible for ERG differences. There are certain technical difficulties, some subtle, that can arise in recording the ERG and these are classified and illustrated in order to facilitate their diagnosis. Forward genetic screens are described, along with abnormal mice that have been generated in a large screen. Several means are suggested for determining if a mouse having an abnormal ERG is a mutant.

Tài liệu tham khảo

Heckenlively JR, Rodriguez JA, Daiger SP (1991) Autosomal dominant sectoral retinitis pigmentosa. Two families with transversion mutation in codon 23 of rhodopsin. Arch Ophthalmol 109:84–91

Gallemore RP, Hughes BA (1998) Light-induced responses of the retinal pigment epithelium. In: Marmor MF, Wolfensberger TJ (eds) Retinal pigment epithelial function and disease. Oxford University Press, New York, pp 175–198

Pacione LR, Szego MJ, Ikeda S, Nishina PM, McInnes RR (2003) Progress toward understanding the genetic and biochemical mechanisms of inherited photoreceptor degenerations. Annu Rev Neurosci 26:657–700

Bruckner R (1951) Slit-lamp microscopy and ophthalmoscopy in rat and mouse. Doc Ophthalmol 5–6:452–554