Influence of Temperature and Methyl Jasmonate on Scenedesmus Incrassulatus
Tóm tắt
The effect of the methyl ester of jasmonic acid (MeJA) in 10 μM concentration was studied on the development of the bacterial contaminants and on the content of some metabolites in Scenedesmus incrassulatus cultivated at temperatures 15, 20, 25, 30, and 36 °C. The number of bacteria on algae cells increased with the rise in temperature. Application of MeJA into nutrient medium inhibited the development of bacterial pathogens more than 3 times at 20 °C, 2.3 times at 30 °C, and 2.6 times at 36 °C without changing the species composition. MeJA caused an increase of the protein content in algae cells. The contents of palmitic and linoleic acids increased with the rise of temperature from 15 to 36 °C. At the same time the contents of linolenic and oleic acid decreased. At low temperatures, cultivation with MeJA induced more significant changes in the composition of C18 acids while at high temperature the changes were more pronounced in C16 acids. Treatment with MeJA decreased the activity of glutamate dehydrogenase at optimal and suboptimal temperatures and increased it at superoptimal temperature. Hence MeJA jasmonate had a positive effect on the tolerance of S. incrassulatus to stress temperatures, which was also demonstrated by better growth.
Tài liệu tham khảo
Arnon, D.: Copper enzymes in isolated chloroplast. Polyphenol oxidase in Beta vulgaris.-Plant Physiol. 24: 1–15, 1949.
Christov, C., Fournadzieva, S., Bozhcova, M., Cherkezov, N., Pilarski, P., Zafirova, T.: Abscisic acid and jasmonates content during the ontogenesis of Scenedesmus acutus.-In: Ordog, V., Szigeti, J., Pulz, O. (ed.): Proceedings of a Conference on Progress in Plant Sciences from Plant Breeding to Growth Regulation. Pp. 155–162. Pannon University of Agricultural Sciences, Mosonmagyarovár 1996.
Creelman, R., Mullet, J.: Biosynthesis and action of jasmonates in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 48: 355–381, 1997.
Georgiev, D., Dilov, C., Avramova, S.: [Buffer nutrient medium and a method for intensive cultivation of green microalgae.]-Hydrobiologia (Sofia) 7: 14–24, 1978. [In Bulg.]
Evans, L., Trewavas, A.: Is algal development controlled by plant growth substances?-J. Phycol. 27: 322–326, 1991.
Harwood, J., Jones, A.: Lipid metabolism in algae.-Adv. bot. Res. 16: 1–53, 1989.
Krupika, M., Dathe, W.: Occurrence of jasmonic acid in the red alga Gelidium latifolium.-Z. Naturforsch. 46 C: 1127–1129, 1991.
Lojda, Z., Gossran, R., Schiebler, T.: Enzyme Histochemistry.-Springer-Verlag, Berlin-Heidelberg-New York 1979.
Lowry, O., Rosebrough, N., Randall, R., Farr, A.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265–270, 1951.
Petkov, G., Furnadjieva, S.: Fatty acids composition of acylolipids from Spirulina platensis.-Comp. rend. Acad. bulg. Sci. 41(1): 103–104, 1988.
Pouneva, I., Christov, C., Zafirova, T.: Effect of jasmonic acid methyl ester on the viability of algae and on the activity of some enzymes.-Comp. rend. Acad. Bulg. Sci. 47(11): 85–87, 1994.
Pouneva, I., Christov, C., Zafirova, T., Todorova, A.: [Effect of methyl jasmonate on the viability of algae stored in collection.]-In: Proceedings of a Jubileum Symposium 100 Years from Birthday of the Acad. Boris Stephanov. Pp. 237–239. Bulgarian Acad. Sci., Sofia 1995.
Reinbothe, S., Mollenhauer, B., Reinbothe, C.: JIPs and RIPs: The regulation of plant gene expression by jasmonates in response to environmental cues and pathogens.-Plant Cell 6: 1197–1209, 1994.
Sembdner, G., Parthier, B.: The biochemistry and the physiological and molecular actions of jasmonates.-Annu. Rev. Plant Physiol. mol. Biol. 44: 569–589, 1993.
Toncheva-Panova, T., Furnadjieva, S., Yordanova, S.: Influence of some growth regulating substances on the Scenedesmus acutus bacterial microflora.-In: Lilov, D., Vassilev, G., Christov, C., Andonova, T. (ed.): Plant Growth Regulators. Vol. II: Pp. 511–514. Bulgarian Acad. Sci., Sofia 1987.
Ueda, J., Miyamoto, K., Sato, T., Momotani, Y.: Identification of jasmonic acid from Euglena gracilis (Z.) as a plant growth regulator.-Agr. biol. Chem. 55: 275–276, 1991a.
Ueda, J., Miyamoto, K., Aoki, M., Hirata, T., Sato, T., Momotani, Y.: Identification of jasmonic acid in Chlorella and Spirulina.-Bull. Univ. Osaka Prefect. B 43: 103–108, 1991b.