Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Kích thích chuỗi enzym oxy hóa thay thế dưới điều kiện căng thẳng muối
Tóm tắt
Bài báo này mô tả tác động của NaCl đối với quá trình hô hấp của các huyền phù tế bào Citrus, cụ thể là sự kích thích của enzym oxy hóa thay thế. Việc tiếp xúc của hai huyền phù tế bào Citrus (các giống Carvalhal tangor và Valencia muộn) với 200 hoặc 400 mM NaCl dẫn đến sự giảm tỷ lệ hô hấp của tế bào. Trong những điều kiện này, tỷ lệ hô hấp giảm ít hơn khi có mặt KCN, cho thấy sự kích thích khả năng của enzym oxy hóa thay thế (AOX). Thêm vào đó, các blot miễn dịch cho thấy sự gia tăng về số lượng protein AOX. Các kháng thể được tạo ra chống lại enzyme Sauromatum guttatum nhận diện dạng khử của enzyme gần vùng 35 kDa. Sự tích lũy protein tương quan với khả năng AOX cao hơn một cách có ý nghĩa được quan sát ở giống Carvalhal tangor.
Từ khóa
#NaCl #hô hấp tế bào #enzym oxy hóa thay thế #Citrus #căng thẳng muốiTài liệu tham khảo
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