Immobilised enzymes: science or art?

Cao, 2003, Immobilised enzymes: carrier-bound or carrier-free?, Curr Opin Biotechnol, 14, 387, 10.1016/S0958-1669(03)00096-X

Bornscheuer, 2003, Immobilizing enzymes: how to create more suitable biocatalysts, Angew Chem Int Ed Engl, 42, 3336, 10.1002/anie.200301664

Dyal, 2003, Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles, J Am Chem Soc, 125, 1684, 10.1021/ja021223n

Blanco, 2004, Functionalization of mesoporous silica for lipase immobilisation characterization of the support and the catalysts, J Mol Catal B Enzymatic, 30, 83, 10.1016/j.molcatb.2004.03.012

Hilal, 2004, Immobilization of cross-linked lipase aggregates within microporous polymeric membranes, J Membr Sci, 238, 131, 10.1016/j.memsci.2004.04.002

Cao L: Carrier-bound Bioimmoblisation. VCH; 2005, in press.

Abian, 2002, Preparation of artificial hyper-hydrophilic micro-environments (polymeric salts) surrounding enzyme molecules. new enzyme derivatives to be used in any reaction medium, J Mol Catal B: Enzymatic, 19, 295, 10.1016/S1381-1177(02)00180-7

Guisan, 2001, Preparation of new lipase derivatives with high activity-stability in anhydrous media: adsorption on hydrophobic supports plus hydrophilization with polyethylenimine, J Mol Catal B: Enzymatic, 11, 817, 10.1016/S1381-1177(00)00011-4

Torres-Bacete, 2001, Stabilization of penicillin V acylase from Streptomyces lavendulae by covalent immobilization, J Chem Technol Biotechnol, 76, 525, 10.1002/jctb.403

Park, 2003, Enhancement of stability of GL-7-ACA acylase immobilised on silica gel modified by epoxide silanization, Process Biochem, 39, 359, 10.1016/S0032-9592(03)00090-6

Hwang, 2004, Stabilisation of Bacillus stearothermophilus lipase immobilised on surface-modified silica gels, Biochem Eng J, 17, 85, 10.1016/S1369-703X(03)00142-6

Cao L, Elzinga J: Crosslinked enzyme aggregates and crosslinker agents therefore. WO 03/066850, 2003.

Wilson, 2004, Co-aggregation of penicillin G acylase and polyionic polymers: an easy methodology to prepare enzyme biocatalysts stable in organic media, Biomacromol, 5, 852, 10.1021/bm0343895

Nguyen, 2002, Stabilization of creatine kinase encapsulated in silicate sol-gel materials and unusual temperature effects on its activity, Chem Mater, 14, 4300, 10.1021/cm020398t

Hernaiz, 2000, Immobilization/stabilization on Eupergit C of the β-galactosidase from B. circulans and an α-galactosidase from Aspergillus oryzae, Enzym Microb Technol, 27, 26, 10.1016/S0141-0229(00)00150-2

Ahmad, 2001, Immobilisation and stabilisation of invertase on Cajanus cajan lectin support, Bioresource Technol, 79, 121, 10.1016/S0960-8524(01)00053-0

Mohapatra, 2000, Immobilization of α-chymotrypsin for use in batch and continuous reactors, J Chem Technol Biotechnol, 75, 519, 10.1002/1097-4660(200007)75:7<519::AID-JCTB262>3.0.CO;2-N

Demers, 2001, Immobilization of native and poly(ethylene glycol)-treated (‘PEGylated’) bovine serum amine oxidase into a biocompatible hydrogel, Biotechnol Appl Biochem, 33, 201, 10.1042/BA20010009

Tatsumoto, 1989, Enhanced stability of glucoamylase through chemical crosslinking, Appl Biochem Biotechnol, 20, 293, 10.1007/BF02936490

Martinek, 1988, Stabilization of enzymes by intramolecular cross-linking using bifunctional reagents, Method Enzymol, 137, 615, 10.1016/0076-6879(88)37057-6

Fernandez-Lafuente, 1995, Strategies for enzyme stabilisation by intramolecular crosslinking with bifunctional reagents, Enzym Microb Technol, 17, 517, 10.1016/0141-0229(94)00090-E

Betancor, 2003, Preparation of a stable biocatalyst of bovine liver catalase using immobilization and post immobilization techniques, Biotechnol Progr, 33, 7938

Betancor, 2004, Prevention of interfacial inactivation of enzymes by coating the enzyme surface with dextran-aldehyde, J Biotechnol, 110, 201, 10.1016/j.jbiotec.2004.02.003

Ferreira, 2002, Influence of different silica derivatives in the immobilization and stabilization of a Bacillus licheniformis protease (subtilisin Carlsberg), J Mol Catal B: Enzymatic, 835, 1

Lei, 2002, Entrapping enzyme in a functionalized nanoporous support, J Am Chem Soc, 124, 11242, 10.1021/ja026855o

Reetz, 2003, Second generation sol-gel encapsulated lipases: robust heterogeneous biocatalysts, Adv Syn Catal, 345, 717, 10.1002/adsc.200303016

Chong, 2004, Design of large-pore mesoporous materials for immobilization of penicillin G acylase biocatalyst, Catalysis Today, 93-95, 293, 10.1016/j.cattod.2004.06.064

Yodoya, 2002, Immobilization of bromelain onto porous copoly(γ-methyl-l-glutamate/l-leucine) beads, Europ Polym J, 39, 173, 10.1016/S0014-3057(02)00192-1

Nouaimi, 2001, Immobilization of trypsin on polyester fleece via different spacers, Enzym Microb Technol, 29, 567, 10.1016/S0141-0229(01)00429-X

Hoa, 2004, Integrated enzyme purification and immobilisation processes with immobilised metal affinity adsorbents, Process Biochem, 39, 1573, 10.1016/S0032-9592(03)00288-7

Liu, 2004, Integration of purification with immobilization of Candida rugosa lipase for kinetic resolution of racemic ketoprofen, J Biotechnol, 110, 209, 10.1016/j.jbiotec.2004.02.008

Martin, 2003, Immobilisation on Eupergit C of cyclodextrin glucosyltransferase (CGTase) and properties of the immobilised biocatalyst, J Mol Catal B: Enzymatic, 21, 299, 10.1016/S1381-1177(02)00264-3

Park, 2003, Immobilization of glutaryl-7-aminocephalosporanic acid acylase on silica gel and enhancement of its stability, Appl Biochem Biotechnol, 104, 185, 10.1385/ABAB:104:3:185

Park, 2002, Immobilization of glutaryl-7-aminocephalosporanic acid acylase on modified silica gel hybrid, J Biosci Bioeng, 94, 218, 10.1263/jbb.94.218

Shchipunova, 2004, A new precursor for the immobilization of enzymes, J Biochem Biophys Method, 58, 25

Kronenburg, 2001, Improvement of enantioselectivity by immobilized imprinting of epoxide hydrolase from Rhodotorula glutinis, J Mol Catal B: Enzymatic, 16, 121, 10.1016/S1381-1177(01)00055-8

Buisson, 2001, Encapsulation of lipases in aerogels, J Non-crystal Solids, 285, 295, 10.1016/S0022-3093(01)00470-7

Furukawa, 2002, Effect of imprinting sol-gel immobilized lipase with chiral template substrates in esterification of (R)-(+)- and (S)-(−)-glycidol, J Mol Catal B: Enzymatic, 17, 23, 10.1016/S1381-1177(01)00076-5

Aoun, 1998, Regioselectivity bromohydroxylation of alkenes catalysed by chloroperoxidase: advantages of the immobilisation of enzyme on talc, J Mol Catal B: Enzmatic, 4, 101, 10.1016/S1381-1177(97)00026-X

Palomo, 2002, Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering: hydrolytic resolution of mandelic acid esters, Enzym Microb Technol, 31, 775, 10.1016/S0141-0229(02)00169-2

Pogorevc, 2002, Selectivity enhancement in enantioselectivity hydrolysis of sec-alkyl sulfates by an alkylsulfatase from Rhodococus rubber DSM 44541, Tetrahedron Asymmetry, 13, 1443, 10.1016/S0957-4166(02)00363-4

Chen, 2002, Synthesis of geranylacetate by esterification with lipase entrapped in hybrid sol-gel formed within non-woven fabric, J Am Oil Chem Soc, 79, 309, 10.1007/s11746-002-0479-5

Palomo, 2004, Enzymatic resolution of (+)-glycidyl butyrate in aqueous media. Strong modulation of the properties of the lipase from Rhizopus Oryzae via immobilisation techniques, Tetrahedron Asymmetry, 15, 1157, 10.1016/j.tetasy.2004.03.003

Fernandez-Lorente, 2001, Modulation of lipase properties in macro-aqueous systems by controlled enzyme immobilization: enantioselective hydrolysis of a chiral ester by immobilized Pseudomonas lipase, Enzym Microb Technol, 28, 389, 10.1016/S0141-0229(00)00324-0

Fernandez-Lorente, 2001, Biocatalyst engineering exerts a dramatic effect on selectivity of hydrolysis catalyzed by immobilized lipases in aqueous medium, J Mol Catal B: Enzymatic, 11, 645, 10.1016/S1381-1177(00)00080-1

Rocchietti, 2002, Influence of the enzyme derivative preparation and substrate structure on the enantioselectivity of penicillin G acylase, Enzym Microb Technol, 31, 88, 10.1016/S0141-0229(02)00070-4

Palomo, 2003, Modulation of Mucor miehei lipase properties via directed immobilization on different hetero-functional epoxy resins. Hydrolytic resolution of (R,S)-2-butyroyl-2-phenylacetic acid, J Mol Catal B: Enzymatic, 21, 201, 10.1016/S1381-1177(02)00224-2

Daryl, 2001, Valentine molecular confinement influences protein structure and enhances thermal protein stability, Protein Sci, 10, 250, 10.1110/ps.36201

Ghanema, 2003, Entrapment of Pseudomonas cepacia lipase with peracetylated β-cyclodextrin in sol-gel: application to the kinetic resolution of secondary alcohols, Tetrahedron Asymmetry, 14, 2547, 10.1016/S0957-4166(03)00550-0

Chen, 2003, Polyvinyl formal resin plates impregnated with lipase entrapped sol-gel polymer for flavour ester synthesis, Enzym Microb Technol, 33, 513, 10.1016/S0141-0229(03)00157-1

Chen, 2003, Sol-gel powders and supported sol-gel polymers for immobilization of lipase in ester synthesis, Enzym Microb Technol, 32, 801, 10.1016/S0141-0229(03)00052-8