Hydration-Enhanced Lubricating Electrospun Nanofibrous Membranes Prevent Tissue Adhesion

Research - Tập 2020 - 2020
Liang Cheng1, Yi Wang2, Guoming Sun3,4, Shizhu Wen2, Lianfu Deng1, Hongyu Zhang2, Wenguo Cui1
1Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai 200025, China
2State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, China
3Affiliated Hospital of Hebei University, Baoding 071000, China
4College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China

Tóm tắt

Lubrication is the key to efficient function of human tissues and has significant impact on the comfort level. However, the construction of a lubricating nanofibrous membrane has not been reported as yet, especially using a one-step surface modification method. Here, bioinspired by the superlubrication mechanism of articular cartilage, we successfully construct hydration-enhanced lubricating nanofibers via one-step in situ grafting of a copolymer synthesized by dopamine methacrylamide (DMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC) onto electrospun polycaprolactone (PCL) nanofibers. The zwitterionic MPC structure provides the nanofiber surface with hydration lubrication behavior. The coefficient of friction (COF) of the lubricating nanofibrous membrane decreases significantly and is approximately 65% less than that of pure PCL nanofibers, which are easily worn out under friction regardless of hydration. The lubricating nanofibers, however, show favorable wear-resistance performance. Besides, they possess a strong antiadhesion ability of fibroblasts compared with pure PCL nanofibers. The cell density decreases approximately 9-fold, and the cell area decreases approximately 12 times on day 7. Furthermore, the in vivo antitendon adhesion data reveals that the lubricating nanofiber group has a significantly lower adhesion score and a better antitissue adhesion. Altogether, our developed hydration-enhanced lubricating nanofibers show promising applications in the biomedical field such as antiadhesive membranes.

Từ khóa


Tài liệu tham khảo

10.1021/acs.chemrev.8b00593

10.1002/adfm.201801114

G. Jiang, L. Luo, L. Tan, J. Wang, S. Zhang, F. Zhang, and J. Jin, “Microsphere-fiber interpenetrated superhydrophobic PVDF microporous membranes with improved waterproof and breathable performance,” ACS Applied Materials & Interfaces, vol. 10, no. 33, pp. 28210–28218, 2018

L. Zang, R. Lin, T. Dou, L. . Lu wang, J. Ma, and L. Sun, “Electrospun superhydrophilic membranes for effective removal of Pb(II) from water,” Nanoscale Advances, vol. 1, no. 1, pp. 389–394, 2019

K. Shi, B. Sun, X. Huang, and P. Jiang, “Synergistic effect of graphene nanosheet and BaTiO3 nanoparticles on performance enhancement of electrospun PVDF nanofiber mat for flexible piezoelectric nanogenerators,” Nano Energy, vol. 52, pp. 153–162, 2018

10.1021/acsami.8b01862

10.1002/adfm.201800514

10.1021/acsami.9b01508

K. Shimomura, B. B. Rothrauff, D. A. Hart, S. Hamamoto, M. Kobayashi, H. Yoshikawa, R. S. Tuan, and N. Nakamura, “Enhanced repair of meniscal hoop structure injuries using an aligned electrospun nanofibrous scaffold combined with a mesenchymal stem cell-derived tissue engineered construct,” Biomaterials, vol. 192, pp. 346–354, 2019

10.1002/adma.201404993

B. M. Gumbiner, “Cell adhesion: the molecular basis of tissue architecture and morphogenesis,” Cell, vol. 84, no. 3, pp. 345–357, 1996

J. K. F. Wong, Y. H. Lui, Z. Kapacee, K. E. Kadler, M. W. J. Ferguson, and D. A. McGrouther, “The cellular biology of flexor tendon adhesion formation: an old problem in a new paradigm,” The American Journal of Pathology, vol. 175, no. 5, pp. 1938–1951, 2009

Y. Özoğul, T. Turkey, A. Baykal, D. Onat, N. Renda, and I. Sayek, “An experimental study of the effect of aprotinin on intestinal adhesion formation,” The American Journal of Surgery, vol. 175, no. 2, pp. 137–141, 1998

J. Yang, M. Chen, M. Wu, K. H. Chao, H. N. Ho, and Y. S. Yang, “Office hysteroscopic early lysis of intrauterine adhesion after transcervical resection of multiple apposing submucous myomas,” Fertility and Sterility, vol. 89, no. 5, pp. 1254–1259, 2008

K. T. Shalumon, C. Sheu, C. H. Chen, S. H. Chen, G. Jose, C. Y. Kuo, and J. P. Chen, “Multi-functional electrospun antibacterial core-shell nanofibrous membranes for prolonged prevention of post-surgical tendon adhesion and inflammation,” Acta Biomaterialia, vol. 72, pp. 121–136, 2018

S. Liu, C. Hu, F. Li, X. J. Li, W. Cui, and C. Fan, “Prevention of peritendinous adhesions with electrospun ibuprofen-loaded poly(l-lactic acid)-polyethylene glycol fibrous membranes,” Tissue Engineering Part A, vol. 19, no. 3-4, pp. 529–537, 2013

X. Zhao, S. Jiang, S. Liu, S. Chen, Z. Y. (. W.). Lin, G. Pan, F. He, F. Li, C. Fan, and W. Cui, “Optimization of intrinsic and extrinsic tendon healing through controllable water-soluble mitomycin-C release from electrospun fibers by mediating adhesion-related gene expression,” Biomaterials, vol. 61, pp. 61–74, 2015

T. Goda, T. Konno, M. Takai, and K. Ishihara, “Photoinduced phospholipid polymer grafting on parylene film: advanced lubrication and antibiofouling properties,” Colloids and Surfaces B: Biointerfaces, vol. 54, no. 1, pp. 67–73, 2007

L. Yan, Y. Xiang, J. Yu, Y. Wang, and W. Cui, “Fabrication of antibacterial and antiwear hydroxyapatite coatings via in situ chitosan-mediated pulse electrochemical deposition,” ACS Applied Materials and Interfaces, vol. 9, no. 5, pp. 5023–5030, 2017

J. Klein, “Hydration lubrication,” Friction, vol. 1, no. 1, pp. 1–23, 2013

S. Jahn, J. Seror, and J. Klein, “Lubrication of articular cartilage,” Annual Review of Biomedical Engineering, vol. 18, pp. 235–258, 2016

S. Chen, J. Zheng, L. Li, and S. Jiang, “Strong resistance of phosphorylcholine self-assembled monolayers to protein adsorption: insights into nonfouling properties of zwitterionic materials,” Journal of the American Chemical Society, vol. 127, no. 41, pp. 14473–14478, 2005

K. Ishihara, N. P. Ziats, B. P. Tierney, N. Nakabayashi, and J. M. Anderson, “Protein adsorption from human plasma is reduced on phospholipid polymers,” Journal of Biomedical Materials Research, vol. 25, no. 11, pp. 1397–1407, 1991

K. Ishihara, H. Nomura, T. Mihara, K. Kurita, Y. Iwasaki, and N. Nakabayashi, “Why do phospholipid polymers reduce protein adsorption?,” Journal of Biomedical Materials Research, vol. 39, no. 2, pp. 323–330, 1998

K. Ishihara, Y. Iwasaki, S. Ebihara, Y. Shindo, and N. Nakabayashi, “Photoinduced graft polymerization of 2-methacryloyloxyethyl phosphorylcholine on polyethylene membrane surface for obtaining blood cell adhesion resistance,” Colloids and Surfaces B: Biointerfaces, vol. 18, no. 3-4, pp. 325–335, 2000

M. Chen, W. H. Briscoe, S. P. Armes, H. Cohen, and J. Klein, “Polyzwitterionic brushes: extreme lubrication by design,” European Polymer Journal, vol. 47, no. 4, pp. 511–523, 2011

K. Matyjaszewski, “Atom transfer radical polymerization (ATRP): current status and future perspectives,” Macromolecules, vol. 45, no. 10, pp. 4015–4039, 2012

J. Chiefari, Y. K. (. B.). Chong, F. Ercole, J. Krstina, J. Jeffery, T. P. T. le, R. T. A. Mayadunne, G. F. Meijs, C. L. Moad, G. Moad, E. Rizzardo, and S. H. Thang, “Living free-radical polymerization by reversible addition−fragmentation chain transfer: the RAFT process,” Macromolecules, vol. 31, no. 16, pp. 5559–5562, 1998

C. Boyer, N. A. Corrigan, K. Jung, D. Nguyen, T. K. Nguyen, N. N. M. Adnan, S. Oliver, S. Shanmugam, and J. Yeow, “Copper-mediated living radical polymerization (atom transfer radical polymerization and copper(0) mediated polymerization): from fundamentals to bioapplications,” Journal of the American Chemical Society, vol. 116, no. 4, pp. 1803–1949, 2016

T. J. Deming, “Mussel byssus and biomolecular materials,” Current Opinion in Chemical Biology, vol. 3, no. 1, pp. 100–105, 1999

K. Zhang, Y. Wang, T. Sun, B. Wang, and H. Zhang, “Bioinspired surface functionalization for improving osteogenesis of electrospun polycaprolactone nanofibers,” Langmuir, vol. 34, no. 50, pp. 15544–15550, 2018

10.1016/j.biomaterials.2016.01.002

Y. Shi, and D. Xiong, “Microstructure and friction properties of PVA/PVP hydrogels for articular cartilage repair as function of polymerization degree and polymer concentration,” Wear, vol. 305, no. 1-2, pp. 280–285, 2013

T. Kurokawa, T. Tominaga, Y. Katsuyama, R. Kuwabara, H. Furukawa, Y. Osada, and J. P. Gong, “Elastic−hydrodynamic transition of gel friction,” Langmuir, vol. 21, no. 19, pp. 8643–8648, 2005

M. Kobayashi, Y. Terayama, N. Hosaka, M. Kaido, A. Suzuki, N. Yamada, N. Torikai, K. Ishihara, and A. Takahara, “Friction behavior of high-density poly(2-methacryloyloxyethyl phosphorylcholine) brush in aqueous media,” Soft Matter, vol. 3, no. 6, pp. 740–746, 2007

T. Maeda, K. Hagiwara, S. Yoshida, T. Hasebe, and A. Hotta, “Preparation and characterization of 2-methacryloyloxyethyl phosphorylcholine polymer nanofibers prepared via electrospinning for biomedical materials,” Journal of Applied Polymer Science, vol. 131, no. 14, pp. 40606–40612, 2014

H. Lee, Y. Lee, A. R. Statz, J. Rho, T. G. Park, and P. B. Messersmith, “Substrate-independent layer-by-layer assembly by using mussel-adhesive-inspired polymers,” Advanced Materials, vol. 20, no. 9, pp. 1619–1623, 2008

Y. Wang, W. Cui, J. Chou, S. Wen, Y. Sun, and H. Zhang, “Electrospun nanosilicates-based organic/inorganic nanofibers for potential bone tissue engineering,” Colloids and Surfaces B: Biointerfaces, vol. 172, pp. 90–97, 2018

Y. Wang, C. Luo, G. Yang, X. Wei, D. Liu, and S. Zhou, “A luteolin-loaded electrospun fibrous implantable device for potential therapy of gout attacks,” Macromolecular Bioscience, vol. 16, no. 11, pp. 1598–1609, 2016

Y. Wang, W. Cui, X. Zhao, S. Wen, Y. Sun, J. Han, and H. Zhang, “Bone remodeling-inspired dual delivery electrospun nanofibers for promoting bone regeneration,” Nanoscale, vol. 11, no. 1, pp. 60–71, 2019

C. Shao, Y. Liu, J. Chi, J. Wang, Z. Zhao, and Y. Zhao, “Responsive inverse opal scaffolds with biomimetic enrichment capability for cell culture,” Research, vol. 2019, article 9783793, –10, 2019

S. Liu, M. Qin, C. Hu, F. Wu, W. Cui, T. Jin, and C. Fan, “Tendon healing and anti-adhesion properties of electrospun fibrous membranes containing bFGF loaded nanoparticles,” Biomaterials, vol. 34, no. 19, pp. 4690–4701, 2013

S. Liu, J. Zhao, H. Ruan, T. Tang, G. Liu, D. Yu, W. Cui, and C. Fan, “Biomimetic sheath membrane via electrospinning for antiadhesion of repaired tendon,” Biomacromolecules, vol. 13, no. 11, pp. 3611–3619, 2012