High resolution melting applications for clinical laboratory medicine

Chateigner-Boutin, 2007, A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons, Nucleic Acids Res., 35, e114, 10.1093/nar/gkm640

Cheng, 2006, Rapid detection and identification of clinically important bacteria by high-resolution melting analysis after broad-range ribosomal RNA real-time PCR, Clin. Chem., 52, 1997, 10.1373/clinchem.2006.069286

Chou, 2005, A comparison of high-resolution melting analysis with denaturing high-performance liquid chromatography for mutation scanning: cystic fibrosis transmembrane conductance regulator gene as a model, Am. J. Clin. Pathol., 24, 330, 10.1309/BF3MLJN8J527MWQY

Chou, L.S., Meadows, C., Wittwer, C.T., Lyon, E., 2005b. Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis. Biotechniques 39, 644, 646, 648 passim.

Dahl, 2007, High-resolution melting for accurate assessment of DNA methylation, Clin. Chem., 53, 1877, 10.1373/clinchem.2007.094854

Dames, 2007, Characterization of aberrant melting peaks in unlabeled probe assays, J. Mol. Diagn., 9, 290, 10.2353/jmoldx.2007.060139

Dames, 2007, Unlabeled probes for the detection and typing of herpes simplex virus, Clin. Chem., 53, 1847, 10.1373/clinchem.2007.090761

Dobrowolski, 2005, Validation of dye-binding/high-resolution thermal denaturation for the identification of mutations in the SLC22A5 gene, Hum. Mutat., 25, 306, 10.1002/humu.20137

Dobrowolski, 2007, Streamlined assessment of gene variants by high resolution melt profiling utilizing the ornithine transcarbamylase gene as a model system, Hum. Mutat., 28, 1133, 10.1002/humu.20558

Dobrowolski, 2007, Mutations in the phenylalanine hydroxylase gene identified in 95 patients with phenylketonuria using novel systems of mutation scanning and specific genotyping based upon thermal melt profiles, Mol. Genet. Metab., 91, 218, 10.1016/j.ymgme.2007.03.010

Dujols, 2006, High-resolution melting analysis for scanning and genotyping, 155

Erali, 2006, Multiplex single-color PCR with amplicon melting analysis for identification of Aspergillus species, Clin. Chem., 52, 1443, 10.1373/clinchem.2006.068510

Erali, 2007, SNP genotyping by unlabeled probe melting analysis, Vol. 429, 199

Fortini, 2007, Optimization of high-resolution melting analysis for low-cost and rapid screening of allelic variants of Bacillus anthracis by multiple-locus variable-number tandem repeat analysis, Clin. Chem., 53, 1377, 10.1373/clinchem.2007.085993

Fukui, 2007, Epidermal growth factor receptor mutation status and clinicopathological features of combined small cell carcinoma with adenocarcinoma of the lung, Cancer Sci., 98, 1714, 10.1111/j.1349-7006.2007.00600.x

Graham, 2005, Distinguishing different DNA heterozygotes by high-resolution melting, Clin. Chem., 51, 1295, 10.1373/clinchem.2005.051516

Grievink, 2008, Identification of ryanodine receptor 1 single-nucleotide polymorphisms by high-resolution melting using the LightCycler 480 System, Anal. Biochem., 374, 396, 10.1016/j.ab.2007.11.019

Gundry, 2003, Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes, Clin. Chem., 49, 396, 10.1373/49.3.396

Herrmann, 2006, Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes, Clin. Chem., 52, 494, 10.1373/clinchem.2005.063438

Herrmann, 2007, Instrument comparison for heterozygote scanning of single and double heterozygotes: a correction and extension of Herrmann et al., Clin Chem 2006;52:494–503, Clin. Chem., 53, 150, 10.1373/clinchem.2006.081240

Herrmann, 2007, Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping, Clin. Chem., 53, 1544, 10.1373/clinchem.2007.088120

Holden, 2007, High-resolution melting amplicon analysis as a method to detect c-kit and platelet-derived growth factor receptor alpha activating mutations in gastrointestinal stromal tumors, Am. J. Clin. Pathol., 128, 230, 10.1309/7TEH56K6WWXENNQY

Hung, 2008, Genotyping of the G1138A mutation of the FGFR3 gene in patients with achondroplasia using high-resolution melting analysis, Clin. Biochem., 41, 162, 10.1016/j.clinbiochem.2007.08.014

Jeffery, 2007, Classification of Mycoplasma synoviae strains using single-strand conformation polymorphism and high-resolution melting-curve analysis of the vlhA gene single-copy region, Microbiology, 153, 2679, 10.1099/mic.0.2006/005140-0

Krypuy, 2006, High resolution melting analysis for the rapid and sensitive detection of mutations in clinical samples: KRAS codon 12 and 13 mutations in non-small cell lung cancer, BMC Cancer, 6, 295, 10.1186/1471-2407-6-295

Krypuy, 2007, High resolution melting for mutation scanning of TP53 exons 5–8, BMC Cancer, 7, 168, 10.1186/1471-2407-7-168

Laurie, 2007, Detection of factor VIII gene mutations by high-resolution melting analysis, Clin. Chem., 53, 2211, 10.1373/clinchem.2007.093781

Liew, 2004, Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons, Clin. Chem., 50, 1156, 10.1373/clinchem.2004.032136

Liew, 2006, Genotyping of human platelet antigens 1 to 6 and 15 by high-resolution amplicon melting and conventional hybridization probes, J. Mol. Diagnostics, 8, 97, 10.2353/jmoldx.2006.050053

Liew, 2007, Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting, Am. J. Clin. Pathol., 127, 341, 10.1309/N7RARXH3623AVKDV

Lin, 2008, Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by high-resolution melting analysis, J. Clin. Microbiol., 46, 1090, 10.1128/JCM.02015-07

Margraf, 2006, Mutation scanning of the RET protooncogene using high-resolution melting analysis, Clin. Chem., 52, 138, 10.1373/clinchem.2005.052951

Margraf, 2006, Masking selected sequence variation by incorporating mismatches into melting analysis probes, Hum. Mutat., 27, 269, 10.1002/humu.20290

Margraf, 2007, RET proto-oncogene genotyping using unlabeled probes, the masking technique, and amplicon high-resolution melting analysis, J. Mol. Diagnostics, 9, 184, 10.2353/jmoldx.2007.060091

Montgomery, 2007, Scanning the cystic fibrosis transmembrane conductance regulator gene using high-resolution DNA melting analysis, Clin. Chem., 53, 1891, 10.1373/clinchem.2007.092361

Nomoto, 2006, Detection of EGFR mutations in archived cytologic specimens of non-small cell lung cancer using high-resolution melting analysis, Am. J. Clin. Pathol., 126, 608, 10.1309/N5PQNGW2QKMX09X7

Odell, 2005, Rapid species identification within the Mycobacterium chelonae-abscessus group by high-resolution melting analysis of hsp65 PCR products, Am. J. Clin. Pathol., 123, 96, 10.1309/WDR082X9FFJBQQGB

Palais, 2005, Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping, Anal. Biochem., 346, 167, 10.1016/j.ab.2005.08.010

Palles, 2008, Identification of genetic variants that influence circulating IGF1 levels: a targeted search strategy, Hum. Mol. Genet., 17, 1457, 10.1093/hmg/ddn034

Pornprasert, 2008, Detection of alpha-thalassemia-1 Southeast Asian type using real-time gap-PCR with SYBR Green1 and high resolution melting analysis, Eur. J. Haematol.

Poulson, 2007, Closed-tube genotyping of apolipoprotein E by isolated-probe PCR with multiple unlabeled probes and high-resolution DNA melting analysis, Biotechniques, 43, 87, 10.2144/000112459

Reed, 2007, High-resolution DNA melting analysis for simple and efficient molecular diagnostics, Pharmacogenomics, 8, 597, 10.2217/14622416.8.6.597

Seipp, 2007, Unlabeled oligonucleotides as internal temperature controls for genotyping by amplicon melting, J. Mol. Diagnostics, 9, 284, 10.2353/jmoldx.2007.060136

Seipp, 2007, Quadruplex genotyping of F5, F2, and MTHFR variants in a single closed tube by high-resolution amplicon melting, Clin. Chem., 54, 108, 10.1373/clinchem.2007.097121

Smith, 2007, Detection of EGFR gene mutations in cytology specimens from patients with non-small cell lung cancer utilizing high-resolution melting amplicon analysis, J. Clin. Pathol., 61, 487, 10.1136/jcp.2007.051425

Snell, 2008, BRCA1 promoter methylation in peripheral blood DNA of mutation negative familial breast cancer patients with a BRCA1 tumour phenotype, Breast Cancer Res., 10, R12, 10.1186/bcr1858

Stephens, 2008, High-resolution melting analysis of the spa repeat region of Staphylococcus aureus, Clin. Chem., 54, 432, 10.1373/clinchem.2007.093658

Takano, 2007, Epidermal growth factor receptor mutation detection using high-resolution melting analysis predicts outcomes in patients with advanced non small cell lung cancer treated with gefitinib, Clin. Cancer Res., 13, 5385, 10.1158/1078-0432.CCR-07-0627

Takano, 2008, Rapid detection of carriers with BRCA1 and BRCA2 mutations using high resolution melting analysis, BMC Cancer, 8, 59, 10.1186/1471-2407-8-59

Uemura, 2007, Rapid and high-resolution detection of IgH gene rearrangements using PCR and melting curve analysis, Int. J. Lab. Hematol., 29, 200, 10.1111/j.1751-553X.2006.00832.x

Vandersteen, 2007, Identifying common genetic variants by high-resolution melting, Clin. Chem., 53, 1191, 10.1373/clinchem.2007.085407

White, 2007, Methylation-sensitive high-resolution melting-curve analysis of the SNRPN gene as a diagnostic screen for Prader–Willi and Angelman Syndromes, Clin. Chem., 53, 1960, 10.1373/clinchem.2007.093351

Willmore, 2004, Detection of c-kit-activating mutations in gastrointestinal stromal tumors by high-resolution amplicon melting analysis, Am. J. Clin. Pathol., 122, 206, 10.1309/4E6UYBY62N2FCA6N

Wittwer, 2005, Nucleic acid techniques, Chapter 37, 1407

Wittwer, 2003, High-resolution genotyping by amplicon melting analysis using LCGreen, Clin. Chem., 49, 853, 10.1373/49.6.853

Wojdacz, 2007, Methylation-sensitive high resolution melting (MS-HRM): a new approach for sensitive and high-throughput assessment of methylation, Nucleic Acids Res., 35, e41, 10.1093/nar/gkm013

Zhou, 2004, High-resolution DNA melting curve analysis to establish HLA genotypic identity, Tissue Antigens, 64, 156, 10.1111/j.1399-0039.2004.00248.x

Zhou, 2005, High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution, Clin. Chem., 51, 1770, 10.1373/clinchem.2005.054924