Heat-killed probiotic bacteria differentially regulate colonic epithelial cell production of human β-defensin-2: dependence on inflammatory cytokines

Beneficial microbes - Tập 5 Số 4 - Trang 483-496 - 2014
Neama Habil1,2,3, Wondwossen Abate3, Jane Beal3, Andrew Foey3
1Baghdad, Iraq
2Foundation of Technical Education (FTE), Alnakabat Street,
3School of Biomedical & Healthcare Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, United Kingdom

Tóm tắt

The inducible antimicrobial peptide human β-defensin-2 (hBD-2) stimulated by pro-inflammatory cytokines and bacterial products is essential to antipathogen responses of gut epithelial cells. Commensal and probiotic bacteria can augment such mucosal defences. Probiotic use in the treatment of inflammatory bowel disease, however, may have adverse effects, boosting inflammatory responses. The aim of this investigation was to determine the effect of selected probiotic strains on hBD-2 production by epithelial cells induced by pathologically relevant pro-inflammatory cytokines and the role of cytokine modulators in controlling hBD-2. Caco-2 colonic intestinal epithelial cells were pre-incubated with heat-killed probiotics, i.e. Lactobacillus casei strain Shirota (LcS) or Lactobacillus fermentum strain MS15 (LF), followed by stimulation of hBD-2 by interleukin (IL)-1β and tumour necrosis factor alpha (TNF-α) in the absence or presence of exogenous IL-10 or anti-IL-10 neutralising antibody. Cytokines and hBD-2 mRNA and protein were analysed by real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay. LcS augmented IL-1β-induced hBD-2, whereas LF enhanced TNF-α- and suppressed IL-1β-induced hBD-2. LF enhanced TNF-α-induced TNF-α and suppressed IL-10, whereas augmented IL-1β-induced IL-10. LcS upregulated IL-1β-induced TNF-α mRNA and suppressed IL-10. Endogenous IL-10 differentially regulated hBD-2; neutralisation of IL-10 augmented TNF-α- and suppressed IL-1β-induced hBD-2. Exogenous IL-10, however, suppressed both TNF-α- and IL-1β-induced hBD-2; LcS partially rescued suppression in TNF-α- and IL-1β-stimulation, whereas LF further suppressed IL-1β-induced hBD-2. It can be concluded that probiotic strains differentially regulate hBD-2 mRNA expression and protein secretion, modulation being dictated by inflammatory stimulus and resulting cytokine environment.

Từ khóa


Tài liệu tham khảo

10.1371/journal.pone.0006285

10.1126/science.1075565

European Cytokine Network, 11, 257

10.1074/jbc.271.6.2935

10.1016/S0952-7915(98)80087-3

10.1084/jem.185.9.1573

10.1172/JCI117394

Journal of Immunology, 156, 1143, 10.4049/jimmunol.156.3.1143

Handbook of macrophages: life cycle, functions and diseases121146

10.1038/nri1180

10.1007/s004410100433

10.4049/jimmunol.1000597

10.3920/BM2011.0027

International Journal of Probiotics and Prebiotics, 7, 1

10.3390/nu5061869

10.1111/j.1472-765X.2010.02796.x

10.1002/ijc.21115

10.1016/S0140-6736(03)13490-3

10.1189/jlb.0111004

10.1002/psc.749

10.1006/meth.2001.1262

10.1146/annurev.immunol.19.1.683

Journal of Immunology, 158, 3673, 10.4049/jimmunol.158.8.3673

10.1189/jlb.0708412

10.1093/ajcn/85.2.488

Selection of a chicken Lactobacillus strain with probiotic properties and its application in poultry production

10.1111/j.1365-2249.2007.03587.x

10.1128/IAI.01563-06

10.4049/jimmunol.179.2.1245

Current Opinions in Gastroenterology, 26, 301

10.1172/JCI114394

10.1136/gut.50.2.191

10.1016/j.febslet.2009.04.039

10.1002/jlb.62.2.217

10.1152/ajplung.1997.272.5.L888

10.4049/jimmunol.173.9.5398

10.1086/523335

10.1128/IAI.72.10.5750-5758.2004

10.1097/00054725-200307000-00001

10.1189/jlb.0904543

Journal of Leukocyte Biology, 68, 9, 10.1189/jlb.68.1.9

Clinical and Diagnostic Laboratory Immunology, 11, 686, 10.1128/CDLI.11.4.686-690.2004

Thông tin
Thông tin xuất bản

Beneficial microbes

Tập 5 Số 4

483-496

Thông tin tác giả