Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System

Molecular Plant - Tập 10 Số 3 - Trang 526-529 - 2017
Jingying Li1, Yongwei Sun1, Jinlu Du1, Yunde Zhao2,3, Lanqin Xia1
1Institute of Crop Sciences (ICS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, China
2National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China
3Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA, 92093-0116, USA

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Tài liệu tham khảo

Char, 2016, An Agrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis in maize, Plant Biotechnol. J.

Gao, 2016, An effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing, Plant Physiol., 171, 1794, 10.1104/pp.16.00663

Komor, 2016, CRISPR-based technologies for the manipulation of eukaryotic genomes, Cell

Komor, 2016, Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage, Nature, 533, 420, 10.1038/nature17946

Li, 2016, Gene replacements and insertions in rice by intron targeting using CRISPR-Cas9, Nat. Plants, 2, 16139, 10.1038/nplants.2016.139

Ma, 2015, A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants, Mol. Plant, 8, 1274, 10.1016/j.molp.2015.04.007

Miao, 2013, Targeted mutagenesis in rice using CRISPR-Cas system, Cell Res., 23, 1233, 10.1038/cr.2013.123

Sun, 2016, Engineering herbicide-resistant rice plants through CRISPR/Cas9-mediated homologous recombination of acetolactate synthase, Mol. Plant, 9, 628, 10.1016/j.molp.2016.01.001

Wang, 2015, Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation, Genome Biol., 16, 144, 10.1186/s13059-015-0715-0

Xie, 2013, RNA-guided genome editing in plants using a CRISPR-Cas system, Mol. Plant, 6, 1975, 10.1093/mp/sst119