Gene Targeting in Aspergillus fumigatus by Homologous Recombination Is Facilitated in a Nonhomologous End- Joining-Deficient Genetic Background

American Society for Microbiology - Tập 5 Số 1 - Trang 212-215 - 2006
Sven Krappmann1, Christoph Sasse1, Gerhard H. Braus1
1Institute of Microbiology & Genetics, Department of Molecular Microbiology and Genetics, Georg-August-University, Göttingen, Germany

Tóm tắt

ABSTRACT The akuA gene encoding the Ku70 component of the nonhomologous end-joining machinery was deleted in the opportunistic pathogen Aspergillus fumigatus . No obvious phenotype could be assessed for the corresponding mutant strain but relative frequencies of homologous recombination were increased as deduced from targeting the laccase-encoding abr2 gene.

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Tài liệu tham khảo

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American Society for Microbiology

Tập 5 Số 1

212-215

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