Fractionation of Rhizobium leguminosarum cells into outer membrane, cytoplasmic membrane, periplasmic, and cytoplasmic components

Journal of Bacteriology - Tập 167 Số 3 - Trang 1083-1085 - 1986
Ruud A. de Maagd1, Ben Lugtenberg1
1Botanical Laboratory, Department of Plant Molecular Biology, State University, 2311 VJ Leiden, The Netherlands

Tóm tắt

Rhizobium leguminosarum cells were separated into four distinct fractions by using density gradient centrifugation for the separation of the outer and cytoplasmic membranes and lysozyme-EDTA treatment of whole cells for the isolation of the periplasmic and cytoplasmic fractions. These methods allowed the subcellular localization of R. leguminosarum proteins.

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Tài liệu tham khảo

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