Đặc điểm của cây rau diếp transgenic mang gen ifn-α2b được tái sinh sau khi chuyển gen bằng Agrobacterium rhizogenes

Cytology and Genetics - Tập 46 - Trang 150-154 - 2012
N. A. Matvieieva1, A. M. Shakhovskij1, M. V. Kuchuk1
1Research Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kiev, Ukraine

Tóm tắt

Rễ “lông” của Lactuca sativa và các cây tái sinh mang gen interferon-α2b đã được thu nhận thông qua việc chuyển gen bằng Agrobacterium rhizogenes. Theo các phân tích PCR và RT-PCR, các cây được nghiên cứu có chứa gen ifn-α2b. Các cây tái sinh khác biệt với cây hoang dại ở chỗ có các đốt thân kéo dài, hình thành thân hoa sớm và màu sắc lá tím dưới điều kiện ánh sáng nhân tạo.

Từ khóa

#Lactuca sativa #cây transgenic #interferon-α2b #Agrobacterium rhizogenes #phân tích PCR #RT-PCR

Tài liệu tham khảo

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