Extension of the Life-Span of Human Endothelial Cells by an Interleukin-1αAntisense Oligomer

American Association for the Advancement of Science (AAAS) - Tập 249 Số 4976 - Trang 1570-1574 - 1990
Jeanette A.M. Maier1, Pamela J. Voulalas1, David Roeder1, Thomas Maciag1
1Laboratory of Molecular Biology, Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, MD 20855.

Tóm tắt

The proliferative potential of human diploid endothelial cells is finite, and cellular senescence in vitro is accompanied by the failure of the endothelial cell to respond to exogenous growth factors. Senescent human endothelial cells were shown to contain high amounts of the transcript for the cytokine interleukin-1α (IL-1α), a potent inhibitor of endothelial cell proliferation in vitro. In contrast, transformed human endothelial cells did not contain detectable IL-1α messenger RNA. Treatment of human endothelial cell populations with an antisense oligodeoxynucleotide to the human IL-1α transcript prevented cell senescence and extended the proliferative lifespan of the cells in vitro. Removal of the IL-1α antisense oligomer resulted in the generation of the senescent phenotype and loss of proliferative potential. These data suggest that human endothelial cell senescence in vitro is a dynamic process regulated by the potential intracellular activity of IL-1α.

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