Expression of luminal and basal cytokeratins in human breast carcinoma

Journal of Pathology - Tập 203 Số 2 - Trang 661-671 - 2004
Dalia M. Abd El‐Rehim1, Sarah Pinder, Claire E. Paish, Joshua A. Bell, Ian O. Ellis, J.F.R. Robertson, Robert I. Nicholson
1Department of Histopathology, Breast Unit, Nottingham City Hospital NHS Trust and University of Nottingham, UK.

Tóm tắt

AbstractWe have examined basal and luminal cell cytokeratin expression in 1944 cases of invasive breast carcinoma, using tissue microarray (TMA) technology, to determine the frequency of expression of each cytokeratin subtype, their relationships and prognostic relevance, if any. Expression was determined by immunocytochemistry staining using antibodies to the luminal cytokeratins (CKs) 7/8, 18 and 19 and the basal markers CK 5/6 and CK 14. Additionally, assessment of α‐smooth muscle actin (SMA) and oestrogen receptor status (ER) was performed. The vast majority of the cases showed positivity for CK 7/8, 18 and 19 indicating a differentiated glandular phenotype, a finding associated with good prognosis, ER positivity and older patient age. In contrast, basal marker expression was significantly related to poor prognosis, ER negativity and younger patient age. Multivariate analysis showed that CK 5/6 was an independent indicator for relapse free interval. We were able to subgroup the cases into four distinct phenotype categories (pure luminal, mixed luminal/basal, pure basal and null), which had significant differences in relation to the biological features and the clinical course of the disease. Tumours classified as expressing a basal phenotype (the combined luminal plus basal and the pure basal) were in a poor prognostic subgroup, typically ER negative in most cases. These findings provide further evidence that breast cancer has distinct differentiation subclasses that have both biological and clinical relevance. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

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Tài liệu tham khảo

10.1007/978-1-4899-5043-7_6

Cooper D, 1985, Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations, Lab Invest, 52, 243

10.1002/j.1460-2075.1982.tb01367.x

10.1177/34.7.2423579

10.1097/01.LAB.0000017371.72714.C5

10.1038/35021093

10.1073/pnas.191367098

10.1111/j.1365-2559.1991.tb00229.x

10.1111/j.1365-2559.1992.tb01032.x

10.1111/j.1365-2559.1994.tb01269.x

10.1007/BF01840834

Blamey RW, 1979, Prognostic factors in breast cancer: the formation of a prognostic index, Clin Oncol, 5, 227

10.1038/bjc.1982.106

10.1038/bjc.1982.62

10.1002/bjs.1800770216

10.1016/S0959-8049(99)00056-8

10.1038/nm0798-844

10.1038/labinvest.3780204

10.1016/S0002-9440(10)63075-1

McCarty KS, 1985, Estrogen receptor analyses: correlation of biochemical and immunohistochemical methods using monoclonal antireceptor antibodies, Arch Pathol Lab Med, 109, 716

10.1006/exmp.2001.2363

10.1097/00019606-200303000-00004

10.1016/S0002-9440(10)64399-4

Rejthar A, 1997, The intermediate filaments and prognostically oriented morphological classification in ductal breast carcinoma, Neoplasma, 44, 370

10.1007/s004280050226

10.1007/BF02899036

10.1093/jnci/80.9.691

10.1016/S0046-8177(88)80520-3

Wetzels RH, 1989, Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast, Am J Pathol, 134, 571

Wetzels RH, 1991, Basal cell‐specific and hyperproliferation‐related keratins in human breast cancer, Am J Pathol, 138, 751

10.1002/(SICI)1096-9896(199608)179:4<386::AID-PATH631>3.0.CO;2-V

10.1016/S0002-9440(10)64476-8

Takei H, 1995, Immunohistochemical analysis of cytokeratin #8 as a prognostic factor in invasive breast carcinoma, Anticancer Res, 15, 1101

Schaller G, 1996, Elevated keratin 18 protein expression indicates a favorable prognosis in patients with breast cancer, Clin Cancer Res, 2, 1879

10.1002/(SICI)1097-0320(19980801)32:4<301::AID-CYTO7>3.0.CO;2-K

10.1056/NEJM198608283150906

10.1002/1097-0142(19860601)57:11<2217::AID-CNCR2820571124>3.0.CO;2-T

10.3109/02841869009126532

10.1046/j.1365-2559.2000.00889.x

Lamovec J, 1989, Adenoid cystic carcinoma of the breast: a histologic, cytologic, and immunohistochemical study, Semin Diagn Pathol, 6, 153

10.1016/S0046-8177(99)90028-X

10.1097/00000478-200002000-00005

10.1097/00129039-200303000-00001

10.1309/G6CTR8MDTFUW19XV

10.1054/bjoc.2001.1869

10.1097/01.LAB.0000038508.86221.B3

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Thông tin xuất bản

Journal of Pathology

Tập 203 Số 2

661-671

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