Expression of hGM-CSF in silk glands of transgenic silkworms using gene targeting vector

Adachi T, Tomita M, Shimizu K, Ogawa S, Yoshizato K (2006) Generation of hybrid transgenic silkworms that express Bombyx mori prolyl-hydroxylase α-subunits and human collagens in posterior silk glands: Production of cocoons that contained collagens with hydroxylated praline residues. J Biotechnol 126:205–219

Burgess AW, Begley CG, Johnson GR, Lopez AF, Williamson DJ, Mermod JJ, Simpson RJ, Schmitz A, DeLamarter JF (1987) Purificatio and properties of bacterially synthesized human granulocyte-macrophage colony-stimulating factor. Blood 69:43–51

David AO, Peter WA (1996) Transposable elements and gene transformation in drosophilid insects. Insect Biochem Mol Biol 26:739–753

Denning C, Priddle H (2003) New frontiers in gene targeting and cloning: success, application and challenges in domestic animals and human embryonic stem cells. Reproduction 126:1–11

Dexter TM (1984) Blood cell development: the message in the medium. Nature 309:746–747

Elick TA, Bauser CA, Fraser MJ (1996) Excision of the piggyBac transposable element in vitro is a precise event that is enhanced by the expression of its encoded transposase. Genetica 98:33–41

Evans MJ, CarIton MBL, Russ AP (1997) Gene trapping and functional genomics. Trends Gene 13:370–374

Fraser MJ, Ciszczon T, Elick T, Bauser C (1996) Precise excision of TTAA-specific lepidopteran transposons piggyBac(IFP2) and tagalong(TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera. Insect Mol Biol 5:141–151

Gong CL, Xue RY, Cao GL, Shi XY, Shen WD (2005) hGM-CSF expression by using recombinant BmNPV with inactivated chitinase and cathepsin genes. Prog Biochem Biophy 32:947–952

Guo XY, Zhou ZY, Feng LC, Wang L, Lu C, Xiang ZH (2001) Sperm-mediated gene transformation of silkworm. Prog Biochem Biophys 28:423–425

Hino R, Tomita M, Yoshizato K (2006) The generation of germline transgenic silkworms for the production of biologically active recombinant fusion proteins of fibroin and human basic fibroblast growth factor. Biomaterials 27:5715–5724

Hoare J, Waddington S, Thomas HC, Coutelle C, McGarvey MJ (2005) Complement inhibition rescued mice allowing observation of transgene expression following intraportal delivery of baculovirus in mice. Gene Med 7:325–333

Inoue S, Tanaka K, Arisaka F, Kimura S, Ohtomo K, Mizuno S (2000) Silk fibroin of Bombyx mori is secreted, assembling a high molecular mass elementary unit consisting of H-chain, L-chain, and P25, with a 6:6:1 molar ratio. J Biol Chem 275:40517–40528

Inoue S, Tanaka K, Tanaka H, Ohtomo K, Kanda T, Imamura M, Quan GX, Kojima K, Yamashita T, Nakajima T, Taira H, Tamura T, Mizuno S (2004) Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a-1, 2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25. Eur J Biochem 271:1–11

Inoue S, Tanaka K, Imamura M, Quan GX, Kojima K, Tanaka H, Tomita M, Hino R, Yoshizato K, Mizuno S, Tamura T (2005) A fibroin secretion-deficient silkworm mutant, Nd-sD, provides an efficient system for producing recombinant proteins. Insect Biochem Mol Biol 35:51–59

Kenoutis C, Efrose RC, Swevers L, Lavdas AA, Gaitanou M, Matsas R, Iatrou K (2006) Baculovirus-mediated gene delivery into mammalian cells does not alter their transcriptional and differentiating potential but is accompanied by early viral gene expression. J Virol 80:4135–4146

Kodaira K, Takahashi R, Hirabayashi M, Suzuki T, Ueda M (1997) Recombinant human GM-CSF in the milk of transgenic rats. Theriogenology 47:216

Kurihara H, Sezutsu H, Tamura T, Yamada K (2007) Production of an active feline interferon in the cocoon of transgenic silkworms using the fibroin H-chain expression system. Biochem Biophys Res Commun 355:976–980

Li X, Zhao Y, Zhou WL, Cao GL, Xue RY, Gong CL (2009) Expression of hGM-CSF in stably transformed BmN cells of Bombyx mori. Acta Sericologica Sinica 35:302–307

Li YM, Cao GL, Chen HM, Jia HF, Xue RY, Gong CL (2010) Expression of the hGM-CSF in the silk glands of germline of gene-targeted silkworm. Biochem Biophys Res Comm 391:1427–1431

Mansour SL, Thomas KR, Capecchi MR (1988) Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature 336:348–352

Miyajima A, Otsu K, Schreurs J, Bond MW, Abrams J, Arai K (1986) Expression of murine and human gramulocyte-macrophage colony-stimulating factors in S.cerevisiae: mutageneses of the potential glycosylation sites. EMBO J 5:1193–1197

Nishijima I, Nakahata R, Watanabe S, Tsuji K, Tanaka I, Hirabayashi Y, Inoue T, Arai K (1997) Hematopoietic and lynlphopoietic responses in human granulocyte—macrophage colony—stimulating factor(GM—CSF)receptor transgenic mice injected with human GM—CSF. Blood 90:1031–1038

Ogawa S, Tomita M, Shimizu K, Yoshizato K (2007) Generation of transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon: production of recombinant human serum albumin. J Biotech 128:531–544

Ryoo ZY, Kim MO, Kim KE, Bahk YY, Lee JW, Park SH, Kim JH, Byun SJ, Hwang HY, Youn J, Kim TY (2001) Expression of recombinant human granulocyte macrophage-colony stimulating factor (hGM-CSF) in mouse urine. Transgenic Res 10:193–200

Sardana RK, Alli Z, Dudani A, Tackaberry E, Panahi M, Narayanan M, Ganz P, Altosaar I (2002) Biological activity of human granulocyte-macrophage colony stimulating factor is maintained in a fusion with seed glutelin peptide. Transgenic Res 11:521–531

Shi X, Qin J, Zhu J, Zhu D (1996) Expression of biologically active human granulocyte-macrophage colon-stimulating factor in the silkworm (Bombyx mori). Biotechnol Appl Biochem 24:245–249

Takahiro A, Tomita M, Shimizu K, Ogawa S, Yoshizato K (2006) Generation of hybrid transgenic silkworms that express Bombyx mori prolyl-hydroxylase α-subunits and human collagens in posterior silk glands: production of cocoons that contained collagens with hydroxylated praline residues. J Biotech 126:205–219

Tamura T, Thibert C, Royer C, Kanda T, Abraham E, Loukeris TG, Livadras I, Arca B, Zabalou S, Savakis C (2000) Germline transformation of the silkworm Bombyx mori L using a piggyBac transposon-derived vector. Nat Biotechnol 18:81–84

Tanaka K, Kajiyama N, Ishikura K, Waga S, Kikuchi A, Ohtomo K, Takagi T, Mizuno S (1999) Determination of the site of disulfide linkage between heavy and light chains of silk fbroin produced by Bombyx mori. Biochim Biophys Acta 1432:92–103

Tatematsu K, Kobayashi I, Uchino K, Sezutsu H, Iizuka T, Yonemura N, Tamura T (2010) Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori. Transgenic Res 19(3):473–487

Tomita M, Munetsuna H, Sato T, Adachi T, Hino R, Hayashi M, Shimizu K, Nakamura N, Tamura T, Yoshizato K (2003) Transgenic silkworms produce recombinant human type III procollagen in cocoons. Nat Biotechnol 21:52–56

Wilmut I, Schnieke AE, McWhir J, Kind J, Campbell KHS (2007) Viable offspring derived from fetal and adult mammalian cells. Cloning and Stem Cells 9:3–8

Wu XF, Cao CP (2004) Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination. J Zhejiang Univ Sci 5:644–650

Yamao M, Katayama N, Nakazawa H, Yamakawa M, Hayashi Y, Hara S, Kamei K, Mori H (1999) Gene targeting in the silkworm by use of a baculovirus. Genes Dev 13:511–516

Zhang F, Zhao Y, Chen X, Xu AY, Huang JT, Lu CD (1999) Fluorescent transgenic silkworm. Acta Biochim Biophys Sin 31:119–123

Zhang YZ, Chen J, Lv ZB, Nie ZM, Zhang XY, Wu XF (2006) Can 29 kDa rhGM-CSF expressed by Silkworm pupae bioreactor bring into effect as active cytokine through orally administration? Euro J Pharma Sci 28:212–223

Zhang W, Lv Z, Nie Z, Chen GG, Chen J, Sheng Q, Yu W, Jin YF, Wu XG, Zhang YZ (2009) Bioavailability of orally administered rhGM-CSF: a single-dose, randomized, open-label, two-period crossover trial. PLoS ONE 5:5353

Zhao Y, Chen X, Peng WP, Dong L, Huang JT, Lu CD (2001) Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination. Acta Biochim Biophys Sin 3:112–116

Zhao Y, Li X, Cao GL, Xue RY, Gong CL (2009) Expression of hIGF-I in the silk glands of transgenic silkworms and in transformed silkworm cells. Sci Chin Ser C Life Sci 39:677–684