The generation of germline transgenic silkworms for the production of biologically active recombinant fusion proteins of fibroin and human basic fibroblast growth factor

Inoue, 2000, Silk fibroin of Bombyx mori is secreted, assembling a high molecular mass elementary unit consisting of H-chain, L-chain, and P25, with a 6:6:1 molar ratio, J Biol Chem, 275, 40517, 10.1074/jbc.M006897200 Minoura, 1995, Attachment and growth of fibroblast cells on silk fibroin, Biochem Biophys Res Commun, 208, 511, 10.1006/bbrc.1995.1368 Minoura, 1995, Attachment and growth of cultured fibroblast cells on silk protein matrices, J Biomed Mater Res, 29, 1215, 10.1002/jbm.820291008 Gotoh, 1998, Effect of the chemical modification of the arginyl residue in Bombyx mori silk fibroin on the attachment and growth of fibroblast cells, J Biomed Mater Res, 39, 351, 10.1002/(SICI)1097-4636(19980305)39:3<351::AID-JBM2>3.0.CO;2-I Tsubouchi, 2003, Bombyx mori fibroin enhanced the proliferation of cultured human skin fibroblasts, J Insect Biotechnol Sericol, 72, 65 Yamada, 2004, Identification of fibroin-derived peptides enhancing the proliferation of cultured human skin fibroblasts, Biomaterials, 25, 467, 10.1016/S0142-9612(03)00540-4 Minoura, 1990, Physico-chemical properties of silk fibroin membrane as a biomaterial, Biomaterials, 11, 430, 10.1016/0142-9612(90)90100-5 Li, 2003, Enzymatic degradation behavior of porous silk fibroin sheets, Biomaterials, 24, 357, 10.1016/S0142-9612(02)00326-5 Sofia, 2001, Functionalized silk-based biomaterials for bone formation, J Biomed Mater Res, 54, 139, 10.1002/1097-4636(200101)54:1<139::AID-JBM17>3.0.CO;2-7 Meinel, 2004, Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds, J Biomed Mater Res, 71A, 25, 10.1002/jbm.a.30117 Karageorgiou, 2004, Bone morphogenetic protein-2 decorated silk fibroin films induce osteogenic differentiation of human bone marrow stromal cells, J Biomed Mater Res, 71A, 528, 10.1002/jbm.a.30186 Aoki, 2003, Culture of chondrocytes in fibroin-hydrogel sponge, Bio-med Mater Eng, 13, 309 Nazarov, 2004, Porous 3-D scaffolds from regenerated silk fibroin, Biomacromolecules, 5, 718, 10.1021/bm034327e Kim, 2004, Structure and properties of silk fibroin hydrogels, Biomacromolecules, 5, 786, 10.1021/bm0345460 Motta, 2004, Fibroin hydrogels for biomedical applications; preparation, characterization and in vitro cell culture studies, J Biomater Sci Polymer Edn, 15, 851, 10.1163/1568562041271075 Hanawa, 1995, New oral dosage form for elder patients: preparation and characterization of silk fibroin gel, Chem Pharm Bull, 43, 284, 10.1248/cpb.43.284 Aebischer, 1989, Basic fibroblast growth factor released from synthetic guidance channels facilitates peripheral nerve regeneration across long nerve gaps, J Neurosci Res, 23, 282, 10.1002/jnr.490230306 Pierce, 1992, Platelet-derived growth factor (BB homodimer), transforming growth factor-beta 1, and basic fibroblast growth factor in dermal wound healing. Neovessel and matrix formation and cessation of repair, Am J Pathol, 140, 1375 Ahrendt, 1998, Angiogenic growth factors: a review for tissue engineering, Tissue Eng, 4, 117, 10.1089/ten.1998.4.117 Tsuboi, 1990, Recombinant basic fibroblast growth factor stimulates wound healing in healing-impaired db/db mice, J Exp Med, 172, 245, 10.1084/jem.172.1.245 Greenhalgh, 1990, PDGF and FGF stimulate wound healing in the genetically diabetic mouse, Am J Pathol, 136, 1235 Fiddes, 1991, Preclinical wound-healing studies with recombinant human basic fibroblast growth factor, Ann NY Acad Sci, 638, 316, 10.1111/j.1749-6632.1991.tb49042.x Albertson, 1993, PDGF and FGF reverse the healing impairment in protein-malnourished diabetic mice, Surgery, 114, 368 Okumura, 1996, Acceleration of wound healing in diabetic mice by basic fibroblast growth factor, Biol Pharm Bull, 19, 530, 10.1248/bpb.19.530 Okumura, 1996, Enhanced angiogenesis and granulation tissue formation by basic fibroblast growth factor in healing-impaired animals, Arzneim-Forsh/Drug Res, 46, 1021 Tanaka, 1996, Mechanism of acceleration of wound healing by basic fibroblast growth factor in genetically diabetic mice, Biol Pharm Bull, 19, 1141, 10.1248/bpb.19.1141 Klingbeil CK, Cesar LB, Fiddes JC. Basic fibroblast growth factor accelerates tissue repair in models of impaired wound healing. In: Clinical and experimental approaches to dermal and epidermal repair: normal and chronic wounds: proceedings of the 3rd international symposium, Miami, 1990. p. 443–58. Okumura, 1996, Effect of basic fibroblast growth factor on wound healing in healing-impaired animal models, Arzneim-Forsh/Drug Res, 46, 547 Mizuno, 2003, Effect of chitosan film containing basic fibroblast growth factor on wound healing in genetically diabetic mice, J Biomed Mater Res, 64, 177, 10.1002/jbm.a.10396 DeBois, 1994, Heparin-fibroblast growth factor-fibrin complex: in vitro and in vivo applications to collagen-based materials, Biomaterials, 15, 665, 10.1016/0142-9612(94)90164-3 Fujisato, 1996, Effect of basic fibroblast growth factor on cartilage regeneration in chondrocyte-seeded collagen sponge scaffold, Biomaterials, 17, 155, 10.1016/0142-9612(96)85760-7 Tabata, 1999, Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor, J Biomater Sci Polym Edn, 10, 79, 10.1163/156856299X00298 Perets, 2003, Enhancing the vascularization of three-dimensional porous alginate scaffolds by incorporating controlled release basic fibroblast growth factor microspheres, J Biomed Mater Res, 65, 489, 10.1002/jbm.a.10542 Wong, 2003, Fibrin-based biomaterials to delivery human growth factors, Thromb Haemost, 89, 573, 10.1055/s-0037-1613389 Tomita, 2003, Transgenic silkworms produce recombinant human type III procollagen in cocoons, Nat Biotechnol, 21, 52, 10.1038/nbt771 Tamura, 2000, Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector, Nat Biotechnol, 18, 81, 10.1038/71978 Kanda, 1991, Microinjection system into the early embryo of the silkworm, Bombyx mori, by using air pressure, Bull Nat Inst Sericult Entomol Sci, 2, 32 Ajisawa, 1998, Dissolution of silk fibroin with calciumchloride/ethanol aqueous solution, J Seric Sci Jpn, 67, 91 Knoerzer, 1989, Expression of synthetic genes encoding bovine and human basic fibroblast growth factors (bFGF), Gene, 75, 21, 10.1016/0378-1119(89)90379-X Heath, 1991, Mutations in the heparin-binding domains of human basic fibroblast growth factor alter its biological activity, Biochemistry, 30, 5608, 10.1021/bi00236a039 Rinas, 1992, Cysteine to serine substitutions in basic fibroblast growth factor: Effect on inclusion body formation and proteolytic susceptibility during in vitro refolding, Bio/Technology, 10, 435, 10.1038/nbt0492-435 Andrades, 2001, Engineering, expression, and renaturation of a collagen-targeted human bFGF fusion protein, Growth Factors, 18, 261, 10.3109/08977190109029115 Ishiyama, 1993, A new tetrazolium salt that produces a highly water-soluble dye, Chem Pharm Bull, 41, 1118, 10.1248/cpb.41.1118 Minagawa, 1960, Electron-microscopic investigation on the degumming of silk fabrics, Sen-I Gakkaishi, Jpn, 16, 507, 10.2115/fiber.16.507 Kajio, 1992, Quantitative chlorimetric assay for basic fibroblast growth factor using bovine endothelial cells and heparin, J Pharm Toxic Methods, 28, 9, 10.1016/1056-8719(92)90059-A O’Reilly DR, Miller LK, Luckow VA. Baculovirus expression vectors: a laboratory manual. New York: Oxford University Press. Inoue, 2005, A fibroin secretion-deficient silkworm mutant, Nd-sD, provides an efficient system for producing recombinant proteins, Insect Biochem Mol Biol, 35, 51, 10.1016/j.ibmb.2004.10.002