Expression of Mycobacterium leprae HSP65 in tobacco and its effectiveness as an oral treatment in adjuvant-induced arthritis

Transgenic Research - Tập 20 - Trang 221-229 - 2010
César Rodríguez-Narciso1, Mayra Pérez-Tapia2, Rosa María Rangel-Cano3, Celio L. Silva4, Mariana Meckes-Fisher5, Rafael Salgado-Garciglia1, Sergio Estrada-Parra2, Rodolfo López-Gómez1, Iris Estrada-García2
1Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mexico
2Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas IPN, México D. F., Mexico
3Deparatamento de Ingeniería Genética, CINVESTAV Campus Guanajuato IPN, Irapuato, Mexico
4Department of Biochemistry and Immunology, Medicine School of Ribeirão Preto, The Centre for Tuberculosis Research , University of São Paulo, São Paulo, Brazil
5Farmacología de Productos Naturales, Unidad Pediátrica, Centro Médico Nacional Siglo XXI, México, D.F., Mexico

Tóm tắt

Transgenic plants are able to express molecules with antigenic properties. In recent years, this has led the pharmaceutical industry to use plants as alternative systems for the production of recombinant proteins. Plant-produced recominant proteins can have important applications in therapeutics, such as in the treatment of rheumatoid arthritis (RA). In this study, the mycobacterial HSP65 protein expressed in tobacco plants was found to be effective as a treatment for adjuvant-induced arthritis (AIA). We cloned the hsp65 gene from Mycobacterium leprae into plasmid pCAMBIA 2301 under the control of the double 35S promoter from cauliflower mosaic virus. Agrobacterium tumefaciens bearing the pChsp65 plasmid was used to transform tobacco plants. Incorporation of the hsp65 gene was confirmed by PCR, reverse transcription-PCR, histochemistry, and western blot analyses in several transgenic lines of tobacco plants. Oral treatment of AIA rats with the HSP65 protein allowed them to recover body weight and joint inflammation was reduced. Our results suggest a synergistic effect between the HSP65 expressed protein and metabolites presents in tobacco plants.

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