Expression of Biologically Active Recombinant Pokeweed Antiviral Protein in Methylotrophic Yeast Pichia pastoris

Irvin, 1975, Purification and partial characterization of the antiviral protein from Phytolacca americana which inhibits eukaryotic protein synthesis, Arch. Biochem, Biophys., 169, 522, 10.1016/0003-9861(75)90195-2 Irvin, 1983, Pokeweed antiviral protein, Pharmacol. Ther., 21, 371, 10.1016/0163-7258(83)90061-X Irvin, 1995, Antiviral proteins from Phytolacca, 65 Dallal, 1978, Enzymatic inactivation of eukaryotic ribosomes by the pokeweed antiviral protein, FEBS Lett., 89, 257, 10.1016/0014-5793(78)80230-0 Gessner, 1980, Inhibition of elongation factor 2-dependent translocation by the pokeweed antiviral protein and ricin, J. Biol. Chem., 255, 3251, 10.1016/S0021-9258(19)85688-0 Montanaro, 1975, Inhibition by ricin of protein synthesis in vitro: Inhibition of the binding of elongation factor 2 and of adenosine diphosphate-ribosylated elongation factor 2 to ribosomes, Biochem. J., 146, 127, 10.1042/bj1460127 Hudak, 1999, Pokeweed antiviral protein accesses ribosomes by binding to L3, J. Biol. Chem., 274, 3854, 10.1074/jbc.274.6.3859 Barbieri, 1997, Polynucleotide:adenosine glycosidase activity of ribosome-inactivating proteins: Effects on DNA, RNA and poly(A), Nucleic Acids Res., 25, 518, 10.1093/nar/25.3.518 Nicolas, 1998, A new class of DNA glycosylase/apyrimidinic lyases that act on specific adenines in single-stranded DNA, J. Biol. Chem., 273, 17216, 10.1074/jbc.273.27.17216 Wang, 1999, Pokeweed antiviral protein cleaves double-stranded supercoiled DNA using the same active site required to depurinate rRNA, Nucleic Acids. Res., 27, 1900, 10.1093/nar/27.8.1900 Rajamohan, 1999, Pokeweed antiviral protein isoforms PAP-I, PAP-II, PAP-III depurinate RNA of human immunodeficiency virus (HIV)-I, Biochem. Biophys. Res. Commun., 260, 453, 10.1006/bbrc.1999.0922 Lin, 1991, Isolation and characterization of a cDNA clone encoding the antiviral protein from Phytolacca americana, Plant Mol. Biol., 17, 609, 10.1007/BF00037047 Monzingo, 1993, The 2.5 Å structure of pokeweed antiviral protein, J. Mol. Biol., 233, 705, 10.1006/jmbi.1993.1547 Irvin, 1993, Pokeweed antiviral protein: Ribosome inactivation and therapeutic applications, Pharmacol. Ther., 55, 279, 10.1016/0163-7258(92)90053-3 Uckun, 1995, Immunotoxins for the treatment of leukemia and lymphoma, Leukemia Lymphoma, 18, 195, 10.3109/10428199509059607 Uckun, 1993, Immunotoxins for the treatment of leukemia, Br. J. Hematol., 85, 435, 10.1111/j.1365-2141.1993.tb03329.x Gaynon, 1998, Childrens cancer group (CCG) initiatives in childhood acute lymphoblastic leukemia (ALL), Int. J. Pediatr. Hematol. Oncol., 5, 99 Gehrz, 1991, Treatment of human cytomegalovirus (HCMV) with noval antiviral immunoconjugates, 353 Ussery, 1977, Inhibition of poliovirus replication by a plant antiviral peptide, Ann. N. Y. Acad. Sci., 284, 431, 10.1111/j.1749-6632.1977.tb21979.x Tomlinson, 1974, The inhibition of infection by cucumber mosaic virus and influenza virus by extracts from Phyltolacca americana, J. Gen. Virol., 22, 225, 10.1099/0022-1317-22-2-225 Aron, 1980, Inhibition of herpes simplex virus multiplication by the pokeweed antiviral protein, Antimicrob. Agents Chemother., 17, 1302, 10.1128/AAC.17.6.1032 Zarling, 1990, Inhibition of HIV replication by pokeweed antiviral protein targeted to CD4+ cells by monoclonal antibodies, Nature, 347, 92, 10.1038/347092a0 Uckun, 1998, TXU (Anti-CD7)-pokeweed antiviral protein as a potent inhibitor of human immunodeficiency virus, Antimicrob.l Agents Chemother., 42, 383, 10.1128/AAC.42.2.383 Uckun, 1999, Toxicity, Biological Activity, and Pharmacokinetics of TXU (anti-CD7)-Pokeweed antiviral protein in chimpanzees and adult patients infected with human immunodeficiency virus (HIV- 1), J. Pharmacol. Exp. Ther., 291, 1301 Kataoka, 1990, Expression of a pokeweed antiviral protein in Escherichia coli and its characterization, FEBS Lett., 320, 31, 10.1016/0014-5793(93)81651-F Hur, 1995, Isolation and characterization of pokeweed antiviral mutation in Saccharomyces cerevisiae: Identification of residues important for toxicity, Proc. Natl. Acad. Sci. USA, 92, 8448, 10.1073/pnas.92.18.8448 Sim, 1991, A recombinant human angiostatin protein inhibits experimental primary and metastatic cancer, Cancer Res., 57, 1329 Boehm, 1999, Disruption of the KEX1 gene in Pichia pastoris allows expression of full-length murine and human endostatin, Yeast, 15, 563, 10.1002/(SICI)1097-0061(199905)15:7<563::AID-YEA398>3.0.CO;2-R Kjeldsen, 1999, Secretory expression and characterization of insulin in Pichia pastoris, Biotechnol. Appl. Biochem., 29, 79 Mohanraj, 1995, Expression of biologically active human vascular endothelial growth factor in yeast, Growth Factors, 12, 17, 10.3109/08977199509003210 Eldin, 1997, High-level secretion of two antibody single chain Fv fragments by Pichia pastoris, J. Immunol. Methods, 21, 67, 10.1016/S0022-1759(96)00213-X Luo, 1995, Vl-linker-Vh orientation-dependent expression of single chain Fv-containing an engineered disulfide-stabilized bond in the framework regions, J. Biochem., 118, 825, 10.1093/oxfordjournals.jbchem.a124986 Kobayashi, 1998, The development of recombinant human serum albumin, Ther. Apher., 2, 257, 10.1111/j.1744-9987.1998.tb00118.x Cregg, 1995, Production of foreign proteins in the yeast Pichia pastoris, Can. J. Bot., 73, S891, 10.1139/b95-336 White, 1995, Large scale, expression, purification and characterization of the smallest active fragment of thrombomodulin: The roles of the sixth domain and of methionine388, Protein Eng., 8, 1177, 10.1093/protein/8.11.1177 Romanos, 1995, Advances in the use of Pichia pastoris for high-level expression, Curr. Opin. Biotechnol., 6, 527, 10.1016/0958-1669(95)80087-5 Sreekrishna, 1997, Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris, Gene, 190, 55, 10.1016/S0378-1119(96)00672-5 Rajamohan, 1999, High-level expression and purification of biologically active recombinant pokeweed antiviral protein, Protein Express. Purif., 16, 359, 10.1006/prep.1999.1084