Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin

American Association for the Advancement of Science (AAAS) - Tập 198 Số 4321 - Trang 1056-1063 - 1977
Keiichi Itakura1, Tadaaki Hirose1, Roberto Crea1, Arthur D. Riggs1, Herbert L. Heyneker2, Francisco Bolívar2, Herbert W. Boyer2
1Division of Biology, City of Hope National Medical Center, Duarte, California 91010.
2Department of Biochemistry and Biophysics, University of California, San Francisco 94143.

Tóm tắt

A gene for somatostatin, a mammalian peptide (14 amino acid residues) hormone, was synthesized by chemical methods. This gene was fused to the Escherichia coli β-galactosidase gene on the plasmid pBR322. Transformation of E. coli with the chimeric plasmid DNA led to the synthesis of a polypeptide including the sequence of amino acids corresponding to somatostatin. In vitro, active somatostatin was specifically cleaved from the large chimeric protein by treatment with cyanogen bromide. This represents the first synthesis of a functional polypeptide product from a gene of chemically synthesized origin.

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American Association for the Advancement of Science (AAAS)

Tập 198 Số 4321

1056-1063

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