Evaluation of prolidase activity and oxidative stress in patients with oral lichen planus and oral lichenoid contact reactions

Journal of Oral Pathology and Medicine - Tập 45 Số 4 - Trang 281-288 - 2016
Şule Batu1, Duygu Ofluoğlu2, Sertan Ergün2, Saman Warnakulasuriya3, Ezel Uslu4, Yegane Güven1, Hakkı Tanyeri2
1Department of Biochemistry, Faculty of Dentistry, Istanbul University, Istanbul, Turkey
2Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, İstanbul University, Istanbul, Turkey
3Department of Oral Medicine and WHO Collaborating Centre for Oral Cancer, King's College London Dental Institute, London, UK
4Department of Biochemistry, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey

Tóm tắt

ObjectiveThe aim of this study was to evaluate prolidase activity and oxidative stress in patients with oral lichen planus (OLP) and oral lichenoid contact reactions (OLCR) using serum and salivary samples and to compare these biomarkers with each other as well as with a group of healthy subjects in order to be able to opine their role in the estimation of OLP and OLCR.Patients and MethodsEighteen recently diagnosed patients with OLP, 32 patients with OLCR and 18 healthy controls with matched periodontal status were recruited to the study. Prolidase activity, lipid peroxidation product malondialdehyde (MDA), sialic acid (SA), and advanced oxidation protein products (AOPPs) levels in both serum and saliva were determined. Additionally, salivary flow rate and its buffering capacity were estimated. Statistical analyses were performed using the chi‐square test, t‐test, Mann–Whitney U‐test, and Spearman's rho correlation coefficient.ResultsNo statistically significant differences were observed between the study groups and the control group regarding to the basic characteristics and the periodontal status (P > 0.05). There were no statistically significant differences between OLP and OLCR groups regarding to the distribution of lesions' type, severity, and location (P > 0.05). No significant differences were found between the two study groups with regard to Prolidase activity, MDA, SA, and AOPPs (P ˃ 0.05), whereas statistically significant differences were found between the two study groups and the control group with regard to all evaluated parameters except of serum prolidase (P ˂ 0.01). Moderate correlation was found between salivary MDA and the OLP/OLCR lesion severity, whereas a weak correlation was observed between serum SA and the OLP/OLCR lesion severity (P ˂ 0.05).ConclusionsThe findings of this study suggest an increased prolidase activity and oxidative stress and imbalance in the antioxidant defense system in biological fluids of patients with OLP and OLCR when compared with the healthy subjects. Both OLP and OLCR patients revealed almost similar prolidase activity and oxidative stress levels although these two conditions have different etiopathogenesis.

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Journal of Oral Pathology and Medicine

Tập 45 Số 4

281-288

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