Đánh giá thành phần hóa thực vật, hoạt động chống oxy hóa và kháng khuẩn của các hạt nano bạc tổng hợp từ dịch chiết lá cây thầu dầu (Ricinus communis)

Springer Science and Business Media LLC - Tập 34 - Trang 606-618 - 2021
Abrham Mintiwab1, P. R. Jeyaramraja2
1Department of Biology, College of Natural and Computational Science, Wolaita Sodo University, Wolaita Sodo, Ethiopia
2Department of Biology, College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia

Tóm tắt

Các hạt nano bạc (AgNPs) là các hạt kim loại có kích thước nano, dao động từ 1 đến 100 nm. Nhờ kích thước nhỏ, chúng có thể được sử dụng trong nhiều lĩnh vực như y học, bảo vệ môi trường, truyền thuốc, liệu pháp gen, chế biến thực phẩm, v.v. Do có các hợp chất thực vật cần thiết như flavonoid, tannin và triterpen trong lá cây thầu dầu (Ricinus communis), dịch chiết nước của nó đã được sử dụng trong nghiên cứu này để tổng hợp AgNPs. Sự thay đổi màu sắc từ không màu sang vàng kim, sau đó chuyển sang màu nâu sau 100 phút ủ hỗn hợp “dịch chiết lá cộng với AgNO3” trong bóng tối đã xác nhận sự hình thành AgNPs. Quá trình quang phổ hấp thụ của AgNPs được nghiên cứu trong khoảng 400 đến 700 nm và AgNPs cho thấy có đỉnh hấp thụ tối đa ở 440 nm. Sự liên kết của các hợp chất phenolic và flavonoid với bạc đã được xác nhận bằng cách đo lường hàm lượng của chúng trong AgNPs. Tiềm năng chống oxy hóa của AgNPs tổng hợp được đánh giá thông qua phương pháp thử nghiệm hoạt tính làm sạch gốc tự do DPPH (2, 2-diphenyl-1-picrylhydrazyl). Kết quả thử nghiệm cho thấy hoạt tính làm sạch gốc tự do DPPH của AgNPs cao hơn đáng kể (60,54%) so với dịch chiết lá nước (44,49%) của R. communis với P < 0,05. Hoạt tính kháng khuẩn của AgNPs đối với vi khuẩn Gram dương (Staphylococcus aureus) và hai vi khuẩn Gram âm (Escherichia coli, Pseudomonas aeruginosa) đã được đánh giá bằng phương pháp khuếch tán thạch agar chén-đĩa. Dịch chiết lá R. communis tổng hợp AgNPs thể hiện hoạt tính kháng khuẩn tốt đối với cả vi khuẩn Gram dương và Gram âm. Kết quả cho thấy rằng dịch chiết lá R. communis có thể được sử dụng hiệu quả trong sản xuất AgNPs có tiềm năng chống oxy hóa và kháng khuẩn.

Từ khóa

#hạt nano bạc #Ricinus communis #hoạt động chống oxy hóa #hoạt động kháng khuẩn #hợp chất sinh học

Tài liệu tham khảo

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