Essential Gene Identification and Drug Target Prioritization in Aspergillus fumigatus

PLoS Pathogens - Tập 3 Số 3 - Trang e24
Wenqi Hu1, Susan Sillaots1, Sébastien Lemieux1, John Davison1, Sarah Kauffman2, Anouk Breton1, Annie Linteau1, Chunlin Xin1, Joel Bowman3, Jeff Becker2, Bo Jiang1, Terry Roemer1
1Merck Frosst Center of Fungal Genetics, Montreal, Quebec, Canada
2Department of Microbiology, University of Tennessee, Knoxville, Tennessee, United States of America
3Infectious Diseases, Merck Research Laboratories, Rahway, New Jersey, United States of America

Tóm tắt

Từ khóa


Tài liệu tham khảo

2001, The pathobiology of Aspergillus fumigatus., Trends Microbiol, 9, 382, 10.1016/S0966-842X(01)02104-7

2005, Aspergillus fumigatus: Saprophyte or pathogen?, Curr Opin Microbiol, 8, 385, 10.1016/j.mib.2005.06.017

2005, Aspergillus infections in transplant recipients., Clin Microbiol Rev, 18, 44, 10.1128/CMR.18.1.44-69.2005

1999, Aspergillus fumigatus and aspergillosis., Clin Microbiol Rev, 12, 310, 10.1128/CMR.12.2.310

2005, Advances against aspergillosis., Med Mycol, 43, S1

1999, Genomic profiling of drug sensitivities via induced haploinsufficiency., Nat Genet, 21, 278, 10.1038/6791

1999, Large-scale analysis of the yeast genome by transposon tagging and gene disruption., Nature, 402, 413, 10.1038/46558

2001, An antisense-based functional genomics approach for identification of genes critical for growth of Candida albicans., Nat Biotechnol, 19, 235, 10.1038/85677

2003, Haploinsufficiency-based large-scale forward genetic analysis of filamentous growth in the diploid human fungal pathogen C. albicans., EMBO J, 22, 2668, 10.1093/emboj/cdg256

2006, Control of the C. albicans cell wall damage response by transcriptional regulator Cas5., PLoS Pathog, 2

2003, Large-scale essential gene identification in Candida albicans and applications to antifungal drug discovery., Mol Microbiol, 50, 167, 10.1046/j.1365-2958.2003.03697.x

2002, Characterization of essential genes by parasexual genetics in the human fungal pathogen Aspergillus fumigatus: Impact of genomic rearrangements associated with electroporation of DNA., Genetics, 161, 1077, 10.1093/genetics/161.3.1077

2003, Identification of essential genes in the human fungal pathogen Aspergillus fumigatus by transposon mutagenesis., Eukaryot Cell, 2, 247, 10.1128/EC.2.2.247-255.2003

2004, Gene silencing with RNA interference in the human pathogenic fungus Aspergillus fumigatus., FEMS Microbiol Lett, 237, 317

2003, The Aspergillus nidulans alcA promoter drives tightly regulated conditional gene expression in Aspergillus fumigatus permitting validation of essential genes in this human pathogen., Fungal Genet Biol, 40, 103, 10.1016/S1087-1845(03)00090-2

2005, Doxycycline-regulated gene expression in the opportunistic fungal pathogen Aspergillus fumigatus., BMC Microbiol, 5, 1, 10.1186/1471-2180-5-1

1998, Mapping of the nitrate-assimilation gene cluster (crnA-niiA-niaD) and characterization of the nitrite reductase gene (niiA) in the opportunistic fungal pathogen Aspergillus fumigatus., Curr Genet, 33, 206, 10.1007/s002940050328

1999, The GATA factor AreA is essential for chromatin remodelling in a eukaryotic bidirectional promoter., EMBO J, 18, 1584, 10.1093/emboj/18.6.1584

1999, A developmentally regulated gene cluster involved in conidial pigment biosynthesis in Aspergillus fumigatus., J Bacteriol, 181, 6469, 10.1128/JB.181.20.6469-6477.1999

2005, Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus., Nature, 438, 1151, 10.1038/nature04332

1994, Morphological effects of lipopeptides against Aspergillus fumigatus correlate with activities against (1,3)-beta-D-glucan synthase., Antimicrob Agents Chemother, 38, 1480, 10.1128/AAC.38.7.1480

2001, Identification of two different 14-alpha sterol demethylase-related genes (cyp51A and cyp51B) in Aspergillus fumigatus and other Aspergillus species., J Clin Microbiol, 39, 2431, 10.1128/JCM.39.7.2431-2438.2001

2005, Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibility., Antimicrob Agents Chemother, 49, 2536, 10.1128/AAC.49.6.2536-2538.2005

2006, Evidence from knockout mice against physiologically significant aquaporin 8-facilitated ammonia transport., Am J Physiol Cell Physiol, 291, C417, 10.1152/ajpcell.00057.2006

1996, Attenuated virulence of uridine-uracil auxotrophs of Aspergillus fumigatus., Infect Immun, 64, 4401, 10.1128/IAI.64.10.4401-4405.1996

2004, Discovering modes of action for therapeutic compounds using a genome-wide screen of yeast heterozygotes., Cell, 116, 121, 10.1016/S0092-8674(03)01035-3

2005, Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae., Nature, 438, 1105, 10.1038/nature04341

2001, Membrane and cell wall targets in Aspergillus fumigatus., Drug Resist Update, 4, 38, 10.1054/drup.2001.0185

1994, Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3., J Biol Chem, 26, 2369

1996, Rom1p and ROM2p are GDP/GTP exchange proteins (GEPs) for the Rho1p small GTP binding protein in Saccharomyces cerevisiae., EMBO J, 15, 2196, 10.1002/j.1460-2075.1996.tb00573.x

2003, A point mutation in the 14alpha-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus., Antimicrob Agents Chemother, 47, 1120, 10.1128/AAC.47.3.1120-1124.2003

2003, Mutations in Aspergillus fumigatus resulting in reduced susceptibility to posaconazole appear to be restricted to a single amino acid in the cytochrome P450 14alpha-demethylase., Antimicrob Agents Chemother, 47, 577, 10.1128/AAC.47.2.577-581.2003

2003, Multiple resistance mechanisms among Aspergillus fumigatus mutants with high-level resistance to itraconazole., Antimicrob Agents Chemother, 47, 1719, 10.1128/AAC.47.5.1719-1726.2003

2006, A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors., Proc Natl Acad Sci U S A, 103, 10352, 10.1073/pnas.0601456103

2006, Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end-joining–deficient genetic background., Eukaryot Cell, 5, 212, 10.1128/EC.5.1.212-215.2006

2006, The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus., Eukaryot Cell, 5, 207, 10.1128/EC.5.1.207-211.2006

2000, Depletion of the squalene synthase (ERG9) gene does not impair growth of Candida glabrata in mice., Antimicrob Agents Chemother, 44, 2411, 10.1128/AAC.44.9.2411-2418.2000

2001, In vitro and in vivo effects of 14alpha-demethylase (ERG11) depletion in Candida glabrata., Antimicrob Agents Chemother, 45, 3037, 10.1128/AAC.45.11.3037-3045.2001

1996, Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5′-decarboxylase gene, pyrG, as a unique transformation marker., Curr Genet, 30, 76, 10.1007/s002940050103

1966, The induction and repression of nitrate reductase in the fungus Aspergillus nidulans., Biochim Biophys Acta, 113, 51, 10.1016/S0926-6593(66)80120-0

1994, Characterization of an efficient gene cloning strategy for Aspergillus niger based on an autonomously replicating plasmid: Cloning of the nicB gene of A. niger., Gene, 146, 159, 10.1016/0378-1119(94)90288-7

1983, Transformation by integration in Aspergillus nidulans., Gene, 26, 205, 10.1016/0378-1119(83)90191-9

2002, Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems., J Mol Endocrinol, 29, 23, 10.1677/jme.0.0290023

National Committee for Clinical Laboratory Standards 2002 Reference method for broth dilution antifungal susceptibility testing of filamentous fungi Wayne (Pennsylvania) National Committee for Clinical Laboratory Standards Document M-38A. 7