Enhanced Antifungal Effect of Chitosan/Pepper Tree (Schinus molle) Essential Oil Bionanocomposites on the Viability of Aspergillus parasiticus Spores

Journal of Nanomaterials - Tập 2016 - Trang 1-10 - 2016
Ana G Luque-Alcaraz1, Mario Onofre Cortez‐Rocha1, Carlos Velázquez2, Ana Lilian Acosta-Silva2, Hisila Santacruz‐Ortega3, Armando Burgos‐Hernández1, Waldo Argüelles‐Monal4, Maribel Plascencia‐Jatomea1
1Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Bulevar Luis Encinas y Rosales s/n, Colonia Centro, 83000 Hermosillo, SON, Mexico
2Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Bulevar Luis Encinas y Rosales s/n, Colonia Centro, 83000 Hermosillo, SON, Mexico
3Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, Bulevar Luis Encinas y Rosales s/n, Colonia Centro, 83000 Hermosillo, SON, Mexico
4Centro de Investigación en Alimentación y Desarrollo, A.C., 85480 Guaymas, SON, Mexico

Tóm tắt

Chitosan nanoparticles (CS) and chitosan/pepper tree (Schinus molle) essential oil (CS-EO) bionanocomposites were synthesized by nanoprecipitation method and the in vitro antifungal activity against Aspergillus parasiticus spores was evaluated. The shape and size were evaluated by scanning electron microscopy (SEM) and dynamic light scattering (DLS). The surface charge was determined by assessing the zeta potential and the inclusion of essential oil in bionanocomposites using Fourier transform infrared spectroscopy (FT-IR). The effect on cell viability of the fungus was evaluated using the XTT technique and morphometric analysis by image processing. SEM and DLS analysis indicated that spherical particles with larger diameters for CS-EO biocomposites were observed. Zeta potential values were higher (+11.1 ± 1.60 mV) for CS nanoparticles. Results suggest a chemical interaction between chitosan and pepper tree essential oil. The highest concentration of CS-EO complex caused a larger (40–50%) decrease in A. parasiticus viability. The inclusion of pepper tree oil in CS nanoparticles is a feasible alternative to obtain antifungal biocomposites, where the activity that each compound presents individually is strengthened.

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