Effects of Bothriocephalus acheilognathi on the polarization response of pronephric leucocytes of carp, Cyprinus carpio

Journal of Helminthology - Tập 74 Số 3 - Trang 253-257 - 2000
Pin Nie1, D. Hoole2
1State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences, Wuhan, Hubei 430072, People's Republic of China.
2Keele Univ.

Tóm tắt

AbstractAn in vitro assay was used to examine the effect of Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda: Pseudophyllidea) on the polarization response of pronephric leucocytes of carp, Cyprinus carpio. Leucocytes, isolated from naive, naturally-infected fish and carp injected intraperitoneally with cestode extracts, were exposed to parasite extracts (protein concentrations 0–10.0 μg ml-1), for up to 24 h in the presence or absence of carp serum. In general, polarization responses of the pronephric leucocytes, primarily neutrophils and eosinophils, increased with incubation time although there was no significant difference in the response induced by the different protein concentrations. Differences in the polarization response were, however, observed in naive, naturally infected and injected fish and the cells responded differently in the presence and absence of carp serum. In the absence of carp serum the polarization response of pronephric leucocytes in vitro was significantly reduced with cells obtained from injected and naturally infected fish compared with those obtained from naive carp. This suppression of leucocyte migration was however reduced by the addition of carp serum to the in vitro system. The role of this interaction between the possible suppression of polarization induced by the parasite and stimulation by serum is discussed.

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Tài liệu tham khảo

10.1083/jcb.89.3.585

10.1016/0145-305X(91)90022-Q

10.1002/(SICI)1521-4141(199902)29:02<375::AID-IMMU375>3.0.CO;2-6

10.1111/j.1365-2761.1994.tb00260.x

10.1017/S0031182000067792

10.1016/S0145-305X(94)90355-7

10.1017/S0022149X00015273

Hunt, 1986, Leukotriene B4 induces enhanced migration of fish leucocytes invitro, Immunology, 59, 563

10.1111/j.1095-8649.1985.tb03211.x

10.1017/S0031182000076976

Bauer, 1973, Israel Programme for Scientific Translations, Jerusalem, 115

Sharp, 1992, Lipoxin-induced migration of fish leucocytes, Journal of Leukocyte Biology, 51, 140, 10.1002/jlb.51.2.140

Liao, 1956, Contribution to the biology and control of Bothriocephalusgowkongensis Yeh, a tapeworm parasitic in the young grass carp (Ctenopharyngodon idellus C. and V.), Acta Hydrobiologica Sinica, 2, 129

10.1006/fsim.1994.1019

Van Damme, 1994, The cytokine handbook, 186

10.1017/S0031182099004242

Thông tin
Thông tin xuất bản

Journal of Helminthology

Tập 74 Số 3

253-257

Thông tin tác giả